Bacteriological Profile and Antibiogram of Isolates from Pus Samples in a Tertiary Care Centre

Karki²,

2021

Nepal J Biotechnol

A wound gets infected when the organism gets invaded through the breached skin, proliferated and production of various enzymes, toxins, etc. In order to treat the wound infection, antibiotic susceptibility pattern of organism should be determined before the prescription of the medicine. The present study was conducted from September 2017 to March 2018 with an aim to determine antibiotic susceptibility pattern of Staphylococcus aureus identified from the pus/wound swab among the patients visiting the International Friendship Children's Hospital, Kathmandu, Nepal. Total 270 sample were processed, isolated and identified using standard microbiological procedure and biochemical test. Antibiotic susceptibility test was carried out by using Modified Kirby Bauer's Disc Diffusion Method. Out of total sample, 51.48% (139) showed growth. The growth distribution was found to be high in out-patient department 84.9% (118) than in-patient department 15.1% (21). Among 139 positive growth, 83.5% were gram positive and 16.5% were gram negative. All together 12 different organisms were identified, among which S. aureus was found to be predominant organism 105 (75.5%). S. aureus was found to be sensitive towards Linezolid followed by Doxycycline whereas it was found resistant towards Ciprofloxacin. Among S. aureus identified, 50% were Multidrug resistant (MDR) S. aureus and 55% were Methicillin resistance S. aureus (MRSA). MRSA was found to be sensitive towards Linezolid followed by Doxycycline and resistant towards Ciprofloxacin. The association between MDR and MRSA was found positively significant (i.e. p-value = 0.000). All strains of S. aureus were found to be sensitive towards Vancomycin. 22.86% were double disk diffusion test (D-test) positive. The prevalence of D-test was found to be high in MRSA (75%). The relationship between D-test and MRSA was found to be significantly correlated with each other (r = 0.39). Linezolid, Chloramphenicol, Vancomycin and Doxycycline is a drug of a choice for both S. aureus and MRSA infection.

Section: Discussionsupporting

confidence: 77%

Section: Discussionsupporting

confidence: 76%

Antibiotic Susceptibility Pattern of Staphylococcus aureus Isolated from Pus/Wound Swab from Children Attending International Friendship Children's Hospital

Karki²,

2021

Nepal J Biotechnol

“…Among 95 infected patients, 62 (58.9%) were males and 33 (31.35%) were females. The higher number of male patients than female patients correlates our result with earlier studies [18][19][20][21][22][23]. Age is a significant risk factor in the occurrence of any infection.…”

Section: Discussionsupporting

confidence: 90%

Antibiotic Susceptibility of Bacterial Isolates from Pus Specimens Collected from a General Hospital in Dhaka, Bangladesh

Shewly¹,

Ane²,

Zerin³

et al. 2021

IJRSI

Antibiotic resistance and its rapid spread among pathogenic bacterial isolates are regarded as major public health issues around the world. The study aims to detect pyogenic bacteria in pus samples and assess their antibiotic susceptibilities to various antibiotics commonly employed in chemotherapeutic interventions. This is a retrospective study in which 297 pus samples were obtained from patients in the year 2018 to 2020 for aerobic culture and sensitivity testing. In total, 32% (95) of the samples yielded positive results, whereas, 68% (202) yielded negative results. The most commonly occurring pathogen was E. coli (65) followed by Pseudomonas spp. ( 14), Staphylococcus aureus (9), Klebsiella spp. (4) and Acinetobacter spp. (3). Patients aged 51 and up were the ones who were largely afflicted by the pus infection. Males were shown to be more susceptible to infection than females. Amikacin, Ceftazidime, Meropenem, Cefotaxime, Netilmicin, Mecillinam, and Fosfomycin were the most susceptible drugs for Klebsiella spp., followed by Meropenem for E. coli, Cloxacillin, Doxacillin, Cefotaxime, and Amoxiclave for S. aureus. However, Pefloxacin and Amoxiclav were both effective against Pseudomonas spp., while Levofloxacin, Ciprofloxacin, Doxacillin, Tazobactam+Piperacillin, Imipenem, Netilmicin, and Trimethoprim were effective against Acinetobacter spp. The antibiotic, Ampicillin was found to be resistant to all of the microorganisms tested. In general, most of the isolates were found to be resistant to the majority of the antibiotics. As a result of our research, clinicians will be able to make better decisions when selecting appropriate antibiotics, which aid in patient care and judicious usage, preventing the development of drug resistance in those who are already susceptible.

“…In our study it was observed that men (51.74%) were more prone to acquire pyogenic infections in comparison to women (48.45%). Similar male predominance for developing pyogenic infections was observed in studies by Khanam et al [10] and Khan et al [11] where it was reported to be 56.1% and 56.6% respectively. In our study Gram-negative bacteria were the prevailing isolates 68.15% from pus samples compared to Gram-positive bacteria 31.84%, which is similar to a study by Trojan et al [12].…”

Section: Discussionsupporting

confidence: 85%

Evaluation of Clinico-epidemiology Profile and Susceptibility Pattern of Bacterial Isolates of Purulent Samples in a University Hospital, India

Kar

Dubey

Siddiqui

et al. 2023

JAMB

Background: Patients suffering from trauma, burns or those who have undergone surgical interventions are susceptible to pyogenic infections caused by commensal as well as exogenously acquired pathogens. This study helps us recognize and characterize aerobic bacteria isolated from pyogenic samples and establish antibiotic resistance for clinicians to devise an empirical treatment for patients suffering from similar disease. Methods: This observational study was carried out from November 2019 to May 2020 in the Department of Microbiology at a teaching hospital in northern India and included all patients who presented with suppurative infections during the study period. Results: Total 201 culture positive samples were included in our study of which 80 (40%) samples were of sterile body fluids, 51 (25%) samples were of pus aspirates and the other samples included wound pus and surgical site infection samples. Escherichia coli was the most common pathogen (30.34%) followed by Klebsiella pneumoniae (17.91%) and Pseudomonas spp (13.43%).Sixty eight (33.83%) isolates were multidrug resistant. Enterobacter spp was completely resistant to ciprofloxacin, ceftazidime and ceftriaxone, and 25% sensitive to Cefoperazone-Sulbactam, thus showing the presence of extended spectrum beta lactamase (ESBL) character. We studied the association of multidrug resistance to certain risk factors like previous antibiotic therapy, days of wound before admission, increased length of hospital stay, patient comorbidities like diabetes mellitus, heart disease, chronic liver disease and immunosupression. Conclusions: This study reveals Gram negative bacilli to be more commonly causing pyogenic infections in our patients. Local antibiotic susceptibility profile of these pathogens is essential for empirical treatment.