The use of antibiotics in the poultry and livestock industries for the treatment and prevention of infectious diseases, and as growth promoters in poultry feeds has increased worldwide. Such frequent employment of antibiotics may contribute to the development and dissemination of bacterial antibiotic resistance. The present study was an attempt to isolate drug-resistant bacteria and to screen the probability of having residual antibiotics in the poultry feed samples. Therefore, a total of 18 samples inclusive of starter, grower and finisher of two poultry feed brands of reputed Bangladeshi feed companies were collected and subjected to microbiological analysis, antibiogram and agar well diffusion assay. All the samples contained extended numbers of total viable bacteria and fungi in an average of 108 and 107 cfu/g, respectively. Klebsiellaspp., Pseudomonas spp. and Bacillus spp. were predominantly present in the tested samples. E. coli and Vibrio spp. were also found in most of the samples. Most isolates have been determined to be multidrug-resistant. All the isolates showed resistance against Cefuroxime. Penicillin resistance was found in most of the isolates in greater proportion. Higher rate of resistance was evident against Novobiocin, Cephradine and Rifampicin. However, the bacterial isolates showed sensitivity to Tobramycin, Nalidixic acid and Neomycin. The poultry feed samples, especially starter and finisher of both brands noticeably had significant antimicrobial activity against the laboratory isolates indicative of the probable presence of residual antibiotics which might be used as supplements in the poultry feed samples. Stamford Journal of Microbiology, Vol.10 (1) 2020: 30-34
Owing to the presence of a diverse group of phytochemicals, spices could be potential sources of antibacterial and antioxidant agents. The present investigation was aimed to determine and compare the antimicrobial activities of different spices. A total of 5 spices including cardamom (Seeds, Elettaria cardamomum), cinnamon (Barks, Cinnamomum verum), clove (Flower buds, Syzygium aromaticum), Indian bay leaf or Tejpat (Dried leaves, Cinnamomum tamala), and cumin (Seeds, Cuminum cyminum) were collected. Different extracts (crude, aqueous, ethanolic, and methanolic) of spices were prepared and examined for antimicrobial activity against previously isolated foodborne bacterial isolates. Extracts from the tested spices showed significant inhibitory effects (mostly with >10 mm mean zone of inhibition) as revealed by the agar well diffusion technique. Clove among the tested spices was found to be the prominent one in eliminating foodborne pathogens. Methanolic extracts followed by ethanolic extracts were determined to be most effective against the bacterial isolates when the relative effectivity of different extracts was compared. The frequently encountered minimal inhibitory and bactericidal concentrations of the spices were 12 and 24 mg/mL, respectively. All the spice extracts showed considerable antimicrobial traits which validate their potential and applicability as natural food preservatives and decontaminants.
Antibiotic resistance and its rapid spread among pathogenic bacterial isolates are regarded as major public health issues around the world. The study aims to detect pyogenic bacteria in pus samples and assess their antibiotic susceptibilities to various antibiotics commonly employed in chemotherapeutic interventions. This is a retrospective study in which 297 pus samples were obtained from patients in the year 2018 to 2020 for aerobic culture and sensitivity testing. In total, 32% (95) of the samples yielded positive results, whereas, 68% (202) yielded negative results. The most commonly occurring pathogen was E. coli (65) followed by Pseudomonas spp. ( 14), Staphylococcus aureus (9), Klebsiella spp. (4) and Acinetobacter spp. (3). Patients aged 51 and up were the ones who were largely afflicted by the pus infection. Males were shown to be more susceptible to infection than females. Amikacin, Ceftazidime, Meropenem, Cefotaxime, Netilmicin, Mecillinam, and Fosfomycin were the most susceptible drugs for Klebsiella spp., followed by Meropenem for E. coli, Cloxacillin, Doxacillin, Cefotaxime, and Amoxiclave for S. aureus. However, Pefloxacin and Amoxiclav were both effective against Pseudomonas spp., while Levofloxacin, Ciprofloxacin, Doxacillin, Tazobactam+Piperacillin, Imipenem, Netilmicin, and Trimethoprim were effective against Acinetobacter spp. The antibiotic, Ampicillin was found to be resistant to all of the microorganisms tested. In general, most of the isolates were found to be resistant to the majority of the antibiotics. As a result of our research, clinicians will be able to make better decisions when selecting appropriate antibiotics, which aid in patient care and judicious usage, preventing the development of drug resistance in those who are already susceptible.
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