Antibiotic Susceptibility Pattern of Staphylococcus aureus Isolated from Pus/Wound Swab from Children Attending International Friendship Children's Hospital

Abstract

A wound gets infected when the organism gets invaded through the breached skin, proliferated and production of various enzymes, toxins, etc. In order to treat the wound infection, antibiotic susceptibility pattern of organism should be determined before the prescription of the medicine. The present study was conducted from September 2017 to March 2018 with an aim to determine antibiotic susceptibility pattern of Staphylococcus aureus identified from the pus/wound swab among the patients visiting the International Friendship Children's Hospital, Kathmandu, Nepal. Total 270 sample were processed, isolated and identified using standard microbiological procedure and biochemical test. Antibiotic susceptibility test was carried out by using Modified Kirby Bauer's Disc Diffusion Method. Out of total sample, 51.48% (139) showed growth. The growth distribution was found to be high in out-patient department 84.9% (118) than in-patient department 15.1% (21). Among 139 positive growth, 83.5% were gram positive and 16.5% were gram negative. All together 12 different organisms were identified, among which S. aureus was found to be predominant organism 105 (75.5%). S. aureus was found to be sensitive towards Linezolid followed by Doxycycline whereas it was found resistant towards Ciprofloxacin. Among S. aureus identified, 50% were Multidrug resistant (MDR) S. aureus and 55% were Methicillin resistance S. aureus (MRSA). MRSA was found to be sensitive towards Linezolid followed by Doxycycline and resistant towards Ciprofloxacin. The association between MDR and MRSA was found positively significant (i.e. p-value = 0.000). All strains of S. aureus were found to be sensitive towards Vancomycin. 22.86% were double disk diffusion test (D-test) positive. The prevalence of D-test was found to be high in MRSA (75%). The relationship between D-test and MRSA was found to be significantly correlated with each other (r = 0.39). Linezolid, Chloramphenicol, Vancomycin and Doxycycline is a drug of a choice for both S. aureus and MRSA infection.

Pattern of antibiotic resistance in surgical site infections in a tertiary care hospital of Nepal

“…S. aureus is 100% susceptible to teicoplanin and doxycycline in our study. This is similar to the study of Maharjan et al [23] . In a study of Deka et al [24] , S. aureus were 100% susceptible to clindamycin, which is in contrary to the findings of our study.…”

“…S. aureus is 100% susceptible to teicoplanin and doxycycline in our study. This is similar to the study of Maharjan et al 23 …”

Assessment of the Antibiotic Resistance Characteristics of Staphylococcus aureus Isolated at Da Nang Hospital for Women and Children, Vietnam

“…In this study, the distribution of S. aureus among gender was found to be higher in female than male patients (56.02% vs 43.98%). The finding resembled with the research conducted by Maharjan et al 4 and Pradhan et al 13 The result obtained from the present study was contrast to previous studies 5,14,15 which showed the incidence of S. aureus was much higher in males than in females. The reason could be partly due to the infection rate between genders depending on geography and year of study.…”

“…2 S. aureus was isolated quite commonly in hospitals and medical facilities, causing a serious impact on human health, despite efforts to get rid of bacteria. [3][4][5] Currently, there have been many studies showing that S. aureus is increasingly resistant to antibiotics and spread of multidrug resistant (MDR). Notably, methicillinresistant S. aureus (MRSA) is a global health problem, causing many difficulties in treatment and prognosis.…”

“…7,8 Previous studies also have shown that the prevalence of MRSA strains is higher than that of methicillin-susceptible S. aureus (MSSA). 4,6 In Vietnam, the prevalence of MRSA has been reported to be considerably higher than that in other countries. 3,5,9 In 2019, over 100 000 deaths were attributed to resistance bacteria worldwide, with MRSA accounting for a large proportion of these deaths.…”