Avaliação da atividade antimicrobiana de extratos de Eugenia anomala e Psidium salutare (Myrtaceae) frente à Escherichia coli e Listeria monocytogenes

Abstract

RESUMO:As doenças transmitidas por alimentos ocorrem principalmente devido à ingestão de alimentos contaminados por microrganismos patogênicos, dentre eles a Escherichia coli e Listeria monocytogenes. Uma das alternativas estudadas para minimizar a contaminação de alimentos é o emprego de plantas, ou seus extratos, como agentes antimicrobianos de origem natural em produtos alimentícios. Desta forma o objetivo do presente estudo é fornecer dados científicos a respeito de duas plantas nativas do RS ainda não estudadas, Eugenia anomala e Psidium salutare, visando potencial emprego como agente antimicrobiano natural em alimentos. Para tanto, avaliou-se a atividade antimicrobiana de extratos de E. anomala e P. salutare contra E. coli e L. monocytogenes através da determinação da concentração inibitória mínima (CIM) pelo método de microdiluição em caldo, a capacidade antioxidante dos extratos por meio do método de redução do radical DPPH e a citotoxicidade in vitro empregando células CHO-K1. Os resultados obtidos mostraram que os extratos de acetato de etila e etanólico de ambas as espécies possuem ação antioxidante muito alta, de 94,08% e 93,86%, respectivamente. Apenas o extrato hexânico de P. salutare apresentou ação antimicrobiana moderada (CIM = 312,5 µg/mL). Todos os extratos apresentaram ação citotóxica sendo que os maiores percentuais foram do extrato clorofórmico de E. anomala (77,05%) e hexânico de P. salutare (76,79%), na concentração de 100 µg/mL. Assim, o presente estudo demonstrou que as espécies vegetais estudadas apresentam potencial para emprego como agente antimicrobiano destes microrganismos.Palavras-chave: Concentração Inibitória Mínima, Citotoxicidade in vitro, Atividade Antioxidante.ABSTRACT: Evaluation of the antimicrobial activity of extracts of Eugenia anomala and Psidium salutare (Myrtaceae) against the Escherichia coli and Listeria monocytogenes. The foodborne diseases occur mainly due to the ingestion of food contaminated by pathogenic microorganisms, including Escherichia coli and Listeria monocytogenes. One of the alternatives studied to minimize contamination of food is the use of plants or their extracts as antimicrobial agents naturally occurring in food products. The objective of this study is to provide scientific data on two native plants of RS have not studied Eugenia anomala and Psidium salutare for a potential use as a natural antimicrobial agent in food. To this end, we evaluated the antimicrobial activity of extracts of E. anomala and P. salutare against E. coli and L. monocytogenes by determining the minimum inhibitory concentration (MIC) by the broth microdilution method, the antioxidant capacity of the extract for means DPPH radical reduction method and in vitro cytotoxicity using CHO-K1 cells. The results showed that the ethyl acetate and ethanolic extracts of both species have very high antioxidant activity, of 94.08% and 93.86%, respectively. Only the hexane extract of P. salutare showed a moderate antimicrobial activity (MIC = 312.5 mg/mL). Moreover, all extracts showed ...

Chemical composition of essential oil from Myrcia oblongata DC and potencial antimicrobial, antioxidant and acaricidal activity against Dermanyssus gallinae (Degeer, 1778)

“…This was also observed for the essential oils of Myrcia tomentosa and Myrcia myrtifolia, which showed activity against B. subtilis, S. aureus and other Gram-positive bacteria (SÁ et al, 2012;YOKOMIZO;NAKAOKA-SAKITA, 2014;SIMONETTI et al, 2016). This susceptibility of Gram-positive bacteria can be explained by the presence of a bacterial cell wall that does not restrict the penetration of toxic molecules, whereas Gramnegative bacteria have a barrier system in the outer membrane of the bacterial wall formed by phospholipids, lipopolysaccharides and proteins that are impermeable to many microorganisms (LAMBERT, 2002;MADIGAN et al, 2010).…”

“…Despite the lack of reports on the bioavailability of M. oblongata, the essential oils of the family have recognized importance especially for their antimicrobial activity (SIMONETTI et al, 2016). Studies the antimicrobial properties of plant products have increased in the last decade due to the extensive and undue use of antimicrobials.…”

Ecological anatomy of Eugenia luschnathiana (O.Berg) Klotzsch ex B.D.Jacks. (Myrtaceae) leaves in the Restinga region, state of Ceara

“…Myrtaceae is a widely studied species due to its chemical characteristics of medicinal potential and to the presence of secondary metabolites (ALVARENDA et al, 2015;OLIVEIRA et al, 2016b;SIMONETTI et al, 2016). The great diversity of this family is reflected in the Restinga regions of Brazil (LOURENÇO; BARBOSA, 2012), being well representative in São Gonçalo do Amarante, Ceara (CASTRO et al, 2012).…”

Chemical characterization and antimicrobial activity of Campomanesia aurea against three strains of Listeria monocytogenes

“…Sá et al (2018), evaluated the antimicrobial activity for Campomanesia adamantium against strains of Listeria, including the strain ATCC 7644, which showed a MIC of 0.03125 mg/L. Plants, belonging to the family Myrtaceae are utilized in folk medicine regarding their diuretic action, as anti-fever, anti-rheumatic, and anti-inflammatory agent, and for control of glycemia, amongst many others (Victória et al, 2012;Simonetti, 2016).…”