Chemical composition of essential oil from Myrcia oblongata DC and potencial antimicrobial, antioxidant and acaricidal activity against Dermanyssus gallinae (Degeer, 1778)

Abstract

ABSTRACT:Essential oils have aroused interest in the industrial sector due to the multitude of potential applications, especially with respect their antimicrobial and antioxidant properties, among others. The objective of this study was to determine the chemical composition of the essential oil from the leaves of Myrcia oblongata DC by gas chromatography coupled to mass spectrometry (GC-MS). To evaluate the antioxidant potential of the oil by using the free radical capture method with 2,2-diphenyl-1-picryl hydrazyl (DPPH); to test the oil antimicrobial activity using the broth microdilution method; and to evaluate the repellency and fumigant potential of the oil on Dermanyssus gallinae (Degeer, 1778). The GC-MS analysis resulted in the identification of 30 oil constituents, with the bulk of the composition identified as caryophyllene oxide (22.03%) and trans-verbenol (11.94%). The oil presented moderate antioxidant activity compared to the synthetic antioxidant 2,6-di-tert-butyl-4-hydroxytoluene (BHT). Antimicrobial activity of the essential oil showed an inhibitory activity on Gram-positive bacteria, Enterococcus faecalis, Staphylococcus aureus, Bacillus subtilis and Staphylococcus epidermidis and for the yeast Candida albicans, and showed no activity against Gram-negative bacteria. All concentrations of the essential oil used in the fumigation test on D. gallinae resulted in mortality below 20%. For the repellency test, significant potential was observed for the concentration of 10%.

Antioxidant and Cytotoxic Activities of Myrtaceae Essential Oils Rich in Terpenoids From Brazil

“…13 Moreover, EO of Myrcia oblongata DC., rich in caryophyllene oxide (22.0%) and trans -verbenol (11.9%), was identified as an antioxidant (IC 50 2.8 µg/mL) compared to the synthetic antioxidant BHT (IC 50 1.4 µg/mL). 30…”

Antimicrobial and allelopathic effects of leaves extracts of Myrcia hatschbachii

“…aeruginosa and E. coli. These structural characteristics differ in relation to gram-positive bacteria and can hinder the action of bioactive components (Ayres et al, 2008;Santana et al, 2018).…”

“…Studies on the antimicrobial properties of natural products have increased in the last decade, as microorganisms have become resistant to various drugs used routinely and this motivates the search in plants for alternative therapies with new agents, and moreover, many species are used as antiseptic drugs in folk medicine. Among them, the Myrtaceae family stands out, which was extensively researched for its antimicrobial effects (Özçelik et al, 2011;Santana et al, 2018;Santos et al, 2018).…”

Control of Dermanyssus gallinae (De Geer 1778) and other mites with volatile organic compounds, a review

“…The study, unfortunately, revealed that the addition of thymol to carvacrol decreased the repellent effect of carvacrol. Other studies analysed the different compounds present in a given essential oil by GC-MS experiments, and tested the repellent effect of the oil on PRM by Y-tube olfactometer experiments (Tabari et al ., 2017; Santana et al ., 2018). The essential oil of Artemisia sieberi (Besser) [rich in α -thujone (31.5%), β -thujone (11.92%), camphor (12.3%) and 1,8-cineole (10.09%)] and the essential oil of Myrcia oblongata (DC) [rich in caryophyllene oxide (22.03%) and trans-verbenol (11.94%)] showed a significative repellent effect to D. gallinae.…”

“…The essential oil of Artemisia sieberi (Besser) [rich in α-thujone (31.5%), β-thujone (11.92%), camphor (12.3%) and 1,8-cineole (10.09%)] and the essential oil of Myrcia oblongata (DC) [rich in caryophyllene oxide (22.03%) and transverbenol (11.94%)] showed a significative repellent effect to D. gallinae. Nevertheless, the effect of each of their constituent compounds was not evaluated (Tabari et al, 2017;Santana et al, 2018). Finally, a study by Lee et al (2019) obtained interesting results, in which two essential oils (cinnamon bark and clove bud oils) and some of their constitutive compounds were tested for their repellent abilities.…”