Atividade da isocitrato-liase durante a germinação de sementes de soja

In order to investigate the effect of different doses of essential oil of Moringa oleifera (EOM) on physiological potential and the expression of isoenzymes in bean seeds, an experiment was conducted with two cultivars (Colibri and Campos Gerais) and seven treatments (bactericidal, fungicide and the doses 0, 0.1, 0.2, 0.4 and 0.8% EOM). Germination, first count of germination, germination speed index (GSI), emergence and emergence rate index (ERI) were evaluated in addition to the enzymes esterase (EST), alcohol dehydrogenase (ADH), superoxide dismutase (SOD) and isocitrate lyase (ICL) by electrophoresis. In an experiment conducted in the laboratory, the dose containing 0.4% EOM promoted germination and higher averages for first germination in seeds and GSI in the Colibri cultivar. However, the highest dose used (0.8%) was responsible for the worst physiological performance by the cultivar. For Campos Gerais, increasing doses of essential oil stimulated germination, first count of germination and GSI. In the field, higher doses of essential oil gave higher germination percentages for the Colibri cultivar, not influencing the seeds of Campos Gerais. The ERI was not altered by the treatments with OEM and changes in the molecular forms of the isoenzymes were observed, with low expression values of bands for EST, ICL and ADH-related vigor, the germination of both cultivars, as well as increased expression of SOD, which is associated with reductions in germination.

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“…This fact confirms the suggestion of Martins et al (2000) that an increased activity in this enzyme may be related to more vigorous seeds.…”

Section: Isoenzyme Electrophoresissupporting

confidence: 91%

Physiological performance and electrophoretic pattern of isoenzymes in Phaseolus vulgaris Lam. seeds treated with essential oil of Moringa oleifera Lam

et al. 2016

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“…Martins et al (2000) showed that the enzyme isocitrate-lyase exhibited higher activity levels in the soybean seeds from the cultivar Doko, which normally displays better physiological quality with regard to its seeds than that of other cultivars within the same species. These results support those obtained for the pepper in the current study, in which the highest isocitrate-lyase activity levels were measured in the seeds harvested during the more advanced developmental stages, with high germination and vigor values (Tables 3 and 4).…”

Section: Discussionmentioning

confidence: 99%

Physiological quality and gene expression during the development of habanero pepper (Capsicum chinense Jacquin) seeds

Santos

Pinho

et al. 2015

Genet. Mol. Res.

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“…The activity of this enzyme increases during germination of the seeds, obtaining maximum values at the peak of degraded lipids which are converted into sucrose (Bewley;Black, 1994). Martins et al (2000) observed the importance of ICL and of the glyoxylate cycle for the germination process in soybean seeds and also greater ICL activity in seeds of the Doko cultivar in relation to CAC-1. It is worth noting that seeds of the Doko cultivar tend to show high physiological quality (Braccini et al, 1994;Braccini et al, 1998;MartinsFilho et al, 2001).…”

mentioning

confidence: 81%

“…For that purpose, 600 µL of a solution with 50% diethyl ether + 50% water was used, with homogenization and a 30-minute rest in ice for later centrifuging at 14,000 rpm for 30 min at 4ºC, discarding the supernatant. After extraction and discontinuous electrophoresis, the gels were revealed for the enzymes isocitrate lyase (ICL; EC 4.1.3.1), according to Martins et al (2000) with modifications, esterase (EST; EC 3.1.1.1), malate dehydrogenase (MDH; EC 1.1.1.37), alcohol dehydrogenase (ADH; EC 1.1.1.1), superoxide dismutase (SOD; EC 1.15.1.1) and peroxidase (PRX; EC 1.11.1.7), according to Alfenas et al (2006).…”

mentioning

confidence: 99%

Enzyme activity in soybean seeds produced under foliar application of manganese

et al. 2014

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Several factors affect the quality of soybean seeds, including the mineral nutrition of plants. Manganese (Mn) is an important nutrient because it has as one of its functions the enzyme activation. The aim of this study was to evaluate enzyme activity in seeds of soybean cultivars produced with foliar application of different doses of Mn. The experiment was conducted at the Universidade Federal de Lavras (UFLA) (Federal University of Lavras) in randomized blocks with three replications and 4 x 4 x 2 factorial arrangement consisting of four soybean cultivars, two conventional and its genetically modified RR derived (BRS Celeste and BRS Baliza RR; BRSGO Jataí and BRS Silvânia RR), four doses of Mn via foliar application (0; 200; 400 and 600 g Mn ha -1 ) and two stages of application (R 1 or R 3 ). In the seeds, the expressions of the enzymes esterase (EST), malate dehydrogenase (MDH), alcohol dehydrogenase (ADH), superoxide dismutase (SOD), peroxidase (PRX) and isocitratelyase (ICL) were determined. For evaluation of physiological quality, the germination test and emergence speed index (ESI) were performed. The Mn content in the seeds was also determined. The expression of the enzymes EST, SOD, PRX and ICL in soybean seeds are affected by foliar application of Mn, regardless of the stages of application. In the seeds of the cultivars that showed better physiological quality, Celeste and Baliza RR, greater expressions of the enzymes ADH and ICL and lower expressions of MDH and of PRX were observed.Index terms: Enzymes, Glycine max, quality, micronutrients. RESUMODiversos fatores afetam a qualidade das sementes de soja, dentre eles a nutrição mineral das plantas. O manganês (Mn) é um nutriente importante por ter como uma de suas funções a ativação enzimática. O objetivo, neste trabalho, foi avaliar a atividade enzimática em sementes de cultivares de soja, produzidas com aplicação foliar de diferentes doses de Mn. O experimento foi conduzido na Universidade Federal de Lavras (UFLA), em blocos casualizados, com três repetições e esquema fatorial 4 x 4 x 2, sendo quatro cultivares de soja, duas convencionais e suas derivadas transgênicas RR (BRS Celeste e BRS Baliza RR; BRSGO Jataí e BRS Silvânia RR), quatro doses de Mn via foliar (0; 200; 400 e 600 g Mn ha -1 ) e dois estádios de aplicação (R 1 ou R 3 ). Nas sementes, foram determinadas as expressões das enzimas esterase (EST), malato desidrogenase (MDH), álcool desidrogenase (ADH), superóxido dismutase (SOD), peroxidase (PRX) e isocitrato liase (ICL). Para avaliação da qualidade fisiológica foram realizados os testes de Germinação e de Índice de velocidade de emergência (IVE). Determinou-se, também, o teor de Mn nas sementes. A expressão das enzimas EST, SOD, PRX e ICL em sementes de soja são influenciadas pela aplicação de Mn via foliar, independente do estádio de aplicação. Nas sementes das cultivares, as quais apresentaram melhor qualidade fisiológica, Celeste e Baliza RR, foram constatadas maiores expressões das enzimas ADH e ICL e menores expressões de ...

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Atividade da isocitrato-liase durante a germinação de sementes de soja

Cited by 8 publications

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Physiological performance and electrophoretic pattern of isoenzymes in Phaseolus vulgaris Lam. seeds treated with essential oil of Moringa oleifera Lam

Physiological performance and electrophoretic pattern of isoenzymes in Phaseolus vulgaris Lam. seeds treated with essential oil of Moringa oleifera Lam

Physiological quality and gene expression during the development of habanero pepper (Capsicum chinense Jacquin) seeds

Enzyme activity in soybean seeds produced under foliar application of manganese

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