Aspectos morfológicos e funcionais dos autoimplantes esplênicos em ratos tratados com oxigenio hiperbárico

Paulo, Isabel Cristina Andreatta Lemos; Silva, Alcino Lázaro da; Piras, Cláudio; Ramos, Bernardo Faria; Zanetti, Fernando Roberto; Paulo, Danilo Nagib Salomão

doi:10.1590/s0100-69912010000300011

Cited by 3 publications

(3 citation statements)

References 23 publications

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“…HBO can help the animal lose weight, as demonstrated in a later study 24,29 . In this work, the animals subjected to this treatment lost weight during the first ten days and the group which was not submitted to the treatment gained weight during the same period.…”

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Effect of hyperbaric oxygen therapy in rats with subtotal splenectomy preserving the inferior pole

Paulo

Nunes

et al. 2011

Acta Cir. Bras.

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PURPOSE: To evaluate the effect of hyperbaric oxygen therapy on the survival and weight of rats submitted to subtotal splenectomy and on the viability and growth of the inferior pole. METHODS: Forty Wistar rats underwent subtotal splenectomy with preservation of the inferior pole and distributed into two groups: Group A (n=20) - not treated with hyperbaric oxygen, Group B (n=20) - treated with hyperbaric oxygen. These groups were divided into two subgroups of 10 animals each, according to the time of euthanasia, 15th and 45th days. The survival and weight of the animals were recorded. The inferior pole was measured, weighed and morphologically analyzed. RESULTS: All animals survived. The weight of the animals increased in all subgroups, but decreased on the 10th day in the subgroups treated with hyperbaric oxygen (p<0.001). The viability of the inferior pole was more evident in animals treated on the 15th day, but did not differ on the 45th day. The growth of the inferior pole has not occurred on the 15th day but on the 45th day after surgery in untreated animals (p<0.01) and treated animals (p<0.05). Vascular and cellular increase in treated animals was significantly higher than in untreated ones. CONCLUSION: Hyperbaric oxygen therapy did not affect the survival of animals but reduced their weight. It improved the viability of the inferior splenic pole, but did not interfere with their growth.

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confidence: 82%

Effect of hyperbaric oxygen therapy in rats with subtotal splenectomy preserving the inferior pole

Paulo

Nunes

et al. 2011

Acta Cir. Bras.

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“…Rats submitted to this procedure, followed by hyperbaric oxygenation (HBO) at 100% pure oxygen, in conventional microscopy, showed larger lymphatic follicles, increase of the amount of cells and vessels and more lymphocytic cell proliferation in the inferior pole of the spleen 7,8 . Considering these findings, we proposed to evaluate the immunohistochemistry expression (IHC) of the proliferating cell nuclear antigen (PCNA) which main function is to increase the activity of delta DNA polymerase during DNA replication, event that precedes cell division process 9 .…”

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confidence: 99%

Immunoexpression of proliferating cell nuclear antigen (PCNA) in spleen of splenectomized rats with preservation of inferior pole, submitted to hyperbaric oxygenation

et al. 2013

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PURPOSE:To analyze PCNA immunoexpression on the inferior pole of the spleen of splenectomized rats submitted to hyperbaric oxygenation (HBO). METHODS:Were analyzed fragments of the inferior pole of the spleen of 20 male Wistar rats submitted to splenectomy with preservation of the inferior pole. The rats were divided in two groups: group A (n=10) without HBO and group B (n=10) submitted to HBO at 2, 5 atmospheres per 120 minutes, twice a day for three days and once a day for seven days. The groups were then subdivided in four subgroups: A15 (n=5), with euthanasia on the 15 th day; A45 (n=5), with euthanasia on the 45 th day; B15 (n=5) with euthanasia on the 15 th day and B45 with euthanasia on the 45 th day. Respectively on these days, fragments of the inferior pole of the spleen of all animals were collected and analyzed with the immunohistochemistry technique in order to evaluate PCNA expression. RESULTS:There was an expressive increase in PCNA immunoreactivity in the group B. The 45 day postoperative period resulted in a higher level of positivity than the 15 day postoperative period (p<0.01). CONCLUSION:The quantitative analysis of proliferating cell nuclear antigen positive suggests that hyperbaric oxygenation increases cellular proliferation, contributing to splenic regeneration.

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“…However, those who study the spleen function in lipid metabolism are rare. There is no consensus on the actual mechanism through which the spleen controls serum lipid levels 2,13,14 . The regeneration of the spleen function seems to have a direct relationship with implanted splenic mass 15 , so we decided to perform the allograft spleen in rats, preserving its original functioning spleen, causing the "double spleen".…”

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Effects of splenic allograft in lipid profile of non-splenectomized rats: the immune and metabolic role of the "double spleen"

Gonçalves

Yamaki

Feijó

et al. 2014

Rev. Col. Bras. Cir.

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Gonçalves Gonçalves Gonçalves Gonçalves GonçalvesEffects of splenic allograft in lipid profile of non-splenectomized rats: the immune and metabolic role of the "double spleen"Original Article Original Article Original Article Original Article Original Article Effects of splenic allograft in lipid profile of non-splenectomized Effects of splenic allograft in lipid profile of non-splenectomized Effects of splenic allograft in lipid profile of non-splenectomized Effects of splenic allograft in lipid profile of non-splenectomized Effects of splenic allograft in lipid profile of non-splenectomized rats: the immune and metabolic role of the "double spleen" rats: the immune and metabolic role of the "double spleen" rats: the immune and metabolic role of the "double spleen" rats: the immune and metabolic role of the "double spleen" rats: the immune and metabolic role of the "double spleen"Efeitos do aloenxerto esplênico no lipidograma de ratos não esplenectomizados: Efeitos do aloenxerto esplênico no lipidograma de ratos não esplenectomizados: Efeitos do aloenxerto esplênico no lipidograma de ratos não esplenectomizados: Efeitos do aloenxerto esplênico no lipidograma de ratos não esplenectomizados: Efeitos do aloenxerto esplênico no lipidograma de ratos não esplenectomizados: papel imunológico e metabólico do "baço duplo" papel imunológico e metabólico do "baço duplo" papel imunológico e metabólico do "baço duplo" papel imunológico e metabólico do "baço duplo" papel imunológico e metabólico do "baço duplo" (1), total splenectomy group (2), splenectomy and implantation of allograft group (3) and double spleen group (4). Each group was subdivided into two subgroups: A and B, based on the death of the animals after 30 or 120 days of monitoring. The procedures in groups 2, 3 and 4 were made simultaneously, and splenectomized animals, groups 2 and 3 were donors, respectively, for the animals of groups 3 and 4. In group 4 the spleen was preserved and the animals received implants from the spleens of rats from group 3. The regeneration of splenic tissue was evaluated by macroscopic and microscopic analyzes of the grafts and own spleens, as well as with measurements of VLDL, HDL, LDL, total cholesterol and triglycerides. Results Results ResultsResults Results: after 120 days, Group 4 showed levels of total cholesterol and LDL lower than the other groups. Group 1 had higher levels of lipids. Conclusion Conclusion Conclusion Conclusion Conclusion: The technique of double spleen was effective in the control of lipid metabolism, corroborating the function of the spleen as a reserve of lipids.

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Aspectos morfológicos e funcionais dos autoimplantes esplênicos em ratos tratados com oxigenio hiperbárico

Cited by 3 publications

References 23 publications

Effect of hyperbaric oxygen therapy in rats with subtotal splenectomy preserving the inferior pole

Effect of hyperbaric oxygen therapy in rats with subtotal splenectomy preserving the inferior pole

Immunoexpression of proliferating cell nuclear antigen (PCNA) in spleen of splenectomized rats with preservation of inferior pole, submitted to hyperbaric oxygenation

Effects of splenic allograft in lipid profile of non-splenectomized rats: the immune and metabolic role of the "double spleen"

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