Antitumor Properties of the Essential Oil From the Leaves of Duguetia gardneriana

Abstract

Duguetia gardneriana, popularly known in the Brazilian northeast as "jaquinha", is a species belonging to the family Annonaceae. The aim of this work was to assess the chemical composition and antitumor properties of the essential oil from the leaves of D. gardneriana in experimental models. The chemical composition of the essential oil was analyzed via gas chromatography-flame ionization detector and gas chromatography-mass spectrometry. In vitro cytotoxic activity was determined in cultured tumor cells, and in vivo antitumor activity was assessed in B16-F10-bearing mice. The identified compounds were β-bisabolene (80.99%), elemicin (8.04%), germacrene D (4.15%), and cyperene (2.82%). The essential oil exhibited a cytotoxic effect, with IC50 values of 16.89, 19.16, 13.08, and 19.33 µg/mL being obtained for B16-F10, HepG2, HL-60, and K562 cell lines, respectively. On the other hand, β-bisabolene was inactive in all of the tested tumor cell lines (showing IC50 values greater than 25 µg/mL). The in vivo analysis revealed tumor growth inhibition rates of 5.37-37.52% at doses of 40 and 80 mg/kg/day, respectively. Herein, the essential oil from the leaves of D. gardneriana presented β-bisabolene as the major constituent and showed cytotoxic and antitumor potential.

Antitumour Activity of the Microencapsulation of Annona vepretorum Essential Oil

“…The in vivo antitumour effect was evaluated in C57BL/6 mice inoculated with B16‐F10 melanoma as previously described . Tumour cells (2 × 10 6 cells per 500 μL) were implanted subcutaneously into the left hind groin of mice.…”

Chemical Composition, Cytotoxic, Antimicrobial and Antioxidant Activities of Essential oil from Anthriscus caucalis M. Bieb Grown in China

“…β-Bisabolene, an intermediate in the biosynthesis of many other natural chemical compounds, exhibited potent antitumour properties against B16-F10, HepG2, HL-60 and MCF-7 cells lines [12,13]. Besides, β-Caryophyllene also has been reported to potentiate the cytotoxic activity of paclitaxel against MCF-7, DLD-1 and L-929 tumor cell lines [14].…”

Melipona mondury produces a geopropolis with antioxidant, antibacterial and antiproliferative activities

“…All of the cell lines were kindly provided by Hospital A.C. Camargo (São Paulo, SP, Brazil) and maintained in Roswell Park Memorial Institute-1640 (RPMI-1640; Gibco) medium supplemented with 10% fetal bovine serum (Cultilab), 2 mM l-glutamine (Vetec Química Fina), and 50 µg/mL gentamycin (Novafarma). All cell lines were cultured in cell culture flasks at 37°C in 5% CO 2 and subcultured every 3-4 days to maintain exponential growth (Rodrigues et al 2015) Peripheral blood mononuclear cells (PBMCs) were isolated from healthy, non-smoker donors using a Ficoll-Paque density gradient (GE Healthcare). PBMCs were washed and resuspended at a concentration of 0.3 × 10 6 cells/mL in RPMI 1640 medium supplemented with 20% fetal bovine serum, 2 mM glutamine, and 50 µg/mL gentamycin at 37°C with 5% CO 2 .…”

“…Cell viability was quantified using the Alamar Blue assay, as described previously by Ahmed et al (1994) with minor modifications (Rodrigues et al 2015). Non-tumor and tumor cells were placed in 96-well plates (7 x 10 4 cells/mL for adherent cells or 3 x 10 5 cells/mL for suspended cells in 100 µL of medium) and incubated with EEGP or its fractions (0.39-50 µg/mL) for 72 h at 37°C under a CO 2 (5%) atmosphere.…”