Antioxidant, Cytotoxic and Anti-choline Esterase Activity of Green Silver Nanoparticles Synthesized Using <i>Aspergillus austroafricanus</i> CGJ-B3 (Endophytic Fungus)

Danagoudar, Ananda; Pratap, G.; Shantaram, Manjula; Ghosh, Krishna; Kanade, Santosh R.; Sannegowda, Lokesh Koodlur; Joshi, Chandrashekhar G.

doi:10.1080/22297928.2021.1883477

Cited by 16 publications

(8 citation statements)

References 42 publications

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“…The color was shifted from pale yellow to light brown to intense brown with increasing time of incubation. Researchers have reported color change as the macroscopic observation for the synthesis of silver nanoparticles. − Our findings suggest that synthesis of POAgNPs is attributed to the extracellular enzymes and proteins of metabolites produced from P. oxalicum.…”

Section: Resultsmentioning

confidence: 60%

“…Researchers have reported color change as the macroscopic observation for the synthesis of silver nanoparticles. 24 − 26 Our findings suggest that synthesis of POAgNPs is attributed to the extracellular enzymes and proteins of metabolites produced from P. oxalicum . These enzymes during the electron transfer process reduce the silver ions that gives a brown color to the solution.…”

Section: Resultsmentioning

confidence: 78%

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Green-Based Approach to Synthesize Silver Nanoparticles Using the Fungal Endophyte Penicillium oxalicum and Their Antimicrobial, Antioxidant, and In Vitro Anticancer Potential

et al. 2022

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A green-based approach for the synthesis of silver nanoparticles has gained tremendous attention in biomedical applications. Fungal endophytes have been recognized as a remarkable biological source for the synthesis of potential nanodrugs. The present study focuses on the fabrication of silver nanoparticles using the fungal endophyte Penicillium oxalicum (POAgNPs) associated with the leaf of the Amoora rohituka plant. Sharp UV–visible spectra at 420 nm appeared due to the surface plasmon resonance of POAgNPs and the reduction of silver salt. FT-IR analysis revealed the presence of functional groups of bioactive compounds of P. oxalicum responsible for the reduction of silver salt and validated the synthesis of POAgNPs. A high degree of crystallinity was revealed through XRD analysis, and microscopy-based characterizations such as AFM, TEM, and FESEM showed uniformly distributed, and spherically shaped nanoparticles. Furthermore, POAgNPs showed a potential inhibitory effect against bacterial and fungal strains of pathogenic nature. POAgNPs also exhibited potential antioxidant activity against the synthetically generated free radicals such as DPPH, superoxide, hydroxyl, and nitric oxide with EC50 values of 9.034 ± 0.449, 56.378 ± 1.137, 34.094 ± 1.944, and 61.219 ± 0.69 μg/mL, respectively. Moreover, POAgNPs exhibited cytotoxic potential against the breast cancer cell lines, MDA-MB-231 and MCF-7 with IC50 values of 20.080 ± 0.761 and 40.038 ± 1.022 μg/mL, respectively. POAgNPs showed anticancer potential through inhibition of wound closure and by altering the nuclear morphology of MDA-MB-231 and MCF-7 cells. Further anticancer activity revealed that POAgNPs induced apoptosis in MDA-MB-231 and MCF-7 cells by differential expression of genes related to apoptosis, tumor suppression, and cell cycle arrest and increased the level of Caspase-3. The novel study showed that P. oxalicum-mediated silver nanoparticles exhibit potential biological activity, which can be exploited as nanodrugs in clinical applications.

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Section: Resultsmentioning

confidence: 60%

Section: Resultsmentioning

confidence: 78%

Green-Based Approach to Synthesize Silver Nanoparticles Using the Fungal Endophyte Penicillium oxalicum and Their Antimicrobial, Antioxidant, and In Vitro Anticancer Potential

et al. 2022

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“…Currently, these endophytic fungi are important agents used for the mycosynthesis of AgNPs that are economical and eco‐friendly. Several studies successfully investigated the use of fungal cellular filtrate for the mycosynthesis of AgNPs (Danagoudar et al, 2021; Govindappa, Lavanya, et al, 2020; Hu, Saravanakumar, Jin, & Wang, 2019). The present work is significant because using endophytic fungi obtained from medicinal plants T. fauna to synthesize AgNPs was not reported earlier.…”

Section: Discussionmentioning

confidence: 99%

“…The fungal reduction of metal ions has been related to NADH‐dependent reductases and shuttle quinone extracellular procedures (Duran et al, 2005; Mukherjee et al, 2001). Endophytic fungi are emerging and promising entities in NPs synthesis (Danagoudar et al, 2021; Govindappa et al, 2020) with enhanced applications for prospective future drugs. The bioactive secondary metabolites secreted by endophytic fungi, especially from medicinal plants, can be tailored with NPs as a potent antimicrobial complex, assuming an effective therapeutic effect.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of silver nanoparticles using endophytic Fusarium oxysporum strain NFW16 and their in vitro antibacterial potential

Ilahi

Haleem

Iqbal

et al. 2021

Microscopy Res & Technique

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Nanotechnology has provided a platform for altering, modifying, and developing metal properties to nanoparticles with promising applications. This study aimed to produce functionalized and biocompatible silver nanoparticles (AgNPs) using cellular extracts of endophytic Fusarium oxysporum-NFW16 isolated from Taxus fauna and evaluate its antibacterial potential. Under optimized reaction conditions, welldispersed and extremely stable AgNPs were synthesized in 1 hr. AgNPs were characterized through UV-visible spectrophotometry (at 423 nm), and scanning electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). The obtained AgNPs were spherical, monodispersed, and size was $30-36.1 nm. Strong peaks of XRD (311), ( 220), (200), and (111) matched to silver plane's diffraction facets. FTIR spectra at 1,650, 2,950, and 1,400 cm À1 confirmed the capping of AgNPs with phenolic compounds and compounds having primary amines. The AgNPs showed 100 μg/ml of minimum inhibitory concentration against methicillin-resistant Staphylococcus aureus (MRSA). In addition, AgNPs showed a synergistic effect with both vancomycin and ciprofloxacin against MRSA (25%), Pseudomonas aeruginosa (50%), and pus isolated Escherichia coli (50%).Moreover, AgNPs impregnated cotton and bandage showed in vitro antibacterial potential against American Type Culture Collection and skin-associated clinical pathogenic bacteria. Findings showed that endophytic fungi are the potential source for AgNPs synthesis that are effective against multidrug-resistant bacteria and the development of antimicrobial textile finishes.

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“…There are many studies reporting the isolation of bioactive compounds from series Versicolores , showing the great potential of these fungi for biotechnology, e.g. diphenyl ethers with antimicrobial and cytotoxic activity from A. tennesseensis , beta-glucosidase applicable in cellulose degradation from A. versicolor , or chitinase with antifungal activity from A. griseoaurantiacus ( Kato et al 2015 , Li et al 2018 , Shehata et al 2018 , Sakhri et al 2019 , Danagoudar et al 2021 , Dobolyi et al 2021 , Huang et al 2021 ). Genome sequences for seven species from the series have been deposited to the NCBI GenBank database [accessed 23rd of March 2022].…”

Section: Introductionmentioning

confidence: 99%

Taxonomy of Aspergillus series Versicolores: species reduction and lessons learned about intraspecific variability

Sklenář

Glässnerová

Jurjević³

et al. 2022

Studies in Mycology

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Aspergillus series Versicolores members occur in a wide range of environments and substrates such as indoor environments, food, clinical materials, soil, caves, marine or hypersaline ecosystems. The taxonomy of the series has undergone numerous re-arrangements including a drastic reduction in the number of species and subsequent recovery to 17 species in the last decade. The identification to species level is however problematic or impossible in some isolates even using DNA sequencing or MALDI-TOF mass spectrometry indicating a problem in the definition of species boundaries. To revise the species limits, we assembled a large dataset of 518 strains. From these, a total of 213 strains were selected for the final analysis according to their calmodulin (CaM) genotype, substrate and geography. This set was used for phylogenetic analysis based on five loci (benA, CaM, RPB2, Mcm7, Tsr1). Apart from the classical phylogenetic methods, we used multispecies coalescence (MSC) model-based methods, including one multilocus method (STACEY) and five single-locus methods (GMYC, bGMYC, PTP, bPTP, ABGD). Almost all species delimitation methods suggested a broad species concept with only four species consistently supported. We also demonstrated that the currently applied concept of species is not sustainable as there are incongruences between single-gene phylogenies resulting in different species identifications when using different gene regions. Morphological and physiological data showed overall lack of good, taxonomically informative characters, which could be used for identification of such a large number of existing species. The characters expressed either low variability across species or significant intraspecific variability exceeding interspecific variability. Based on the above-mentioned results, we reduce series Versicolores to four species, namely A. versicolor, A. creber, A. sydowii and A. subversicolor, and the remaining species are synonymized with either A. versicolor or A. creber. The revised descriptions of the four accepted species are provided. They can all be identified by any of the five genes used in this study. Despite the large reduction in species number, identification based on phenotypic characters remains challenging, because the variation in phenotypic characters is high and overlapping among species, especially between A. versicolor and A. creber. Similar to the 17 narrowly defined species, the four broadly defined species do not have a specific ecology and are distributed worldwide. We expect that the application of comparable methodology with extensive sampling could lead to a similar reduction in the number of cryptic species in other extensively studied Aspergillus species complexes and other fungal genera.

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Antioxidant, Cytotoxic and Anti-choline Esterase Activity of Green Silver Nanoparticles Synthesized Using Aspergillus austroafricanus CGJ-B3 (Endophytic Fungus)

Cited by 16 publications

References 42 publications

Green-Based Approach to Synthesize Silver Nanoparticles Using the Fungal Endophyte Penicillium oxalicum and Their Antimicrobial, Antioxidant, and In Vitro Anticancer Potential

Green-Based Approach to Synthesize Silver Nanoparticles Using the Fungal Endophyte Penicillium oxalicum and Their Antimicrobial, Antioxidant, and In Vitro Anticancer Potential

Biosynthesis of silver nanoparticles using endophytic Fusarium oxysporum strain NFW16 and their in vitro antibacterial potential

Taxonomy of Aspergillus series Versicolores: species reduction and lessons learned about intraspecific variability

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