Antimicrobial films produced from chitosan

Bégin, André; Calsteren, Marie‐Rose Van

doi:10.1016/s0141-8130(99)00064-1

Cited by 200 publications

(113 citation statements)

References 15 publications

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“…Chitosan exerts greater bactericidal effects against gram-positive than gram-negative bacteria, and antimicrobial activity is enhanced at low pH values (Senel and McClure, 2004). The positive charges of chitosan influence the negative charges of the bacterial cell surface, due to competition with Ca + for electronegative sites on the membrane without conferring dimensional stability, rendering the membrane leaky (Begin and Calsteren, 1999). Increased propionate production is partially explained by the replacement with gram-negative instead of gram positive bacteria (Russel, 1987).…”

Section: Discussionmentioning

confidence: 99%

Nutrient digestion, microbial protein synthesis, and blood metabolites of Jersey heifers fed chitosan and whole raw soybeans

et al. 2016

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-This study was undertaken to determine the effects of chitosan and whole raw soybean on nutrient intake and total tract digestion, nitrogen utilization, microbial protein synthesis, blood metabolites, and energy balance of dairy heifers. Twelve Jersey heifers (6±0.5 months of age and 139.50±25.56 kg of live weight; mean ± standard deviation) were randomly assigned to a replicated Latin square design with a 2 × 2 factorial arrangement. The experimental period consisted of 14 days of adaptation to diets, six days of sampling, and five days of washout. The experimental diets were: control (CO); chitosan (CHI, inclusion of 2.0 g kg −1 DM of chitosan); whole raw soybean (WS, 163.0 g kg −1 of WS on diet DM basis); and chitosan + whole raw soybean (CHI+WS). Chitosan decreased dry matter and neutral detergent fiber intakes; however, CHI increased DM total tract digestion. An interaction effect was observed on retained nitrogen, which increased when animals were fed CHI+WS compared with CO or CHI, but did not differ from that of animals fed WS. Chitosan decreased microbial nitrogen and crude protein flow of heifers. Energy balance was improved when heifers received diets containing WS. Efficiency of energy utilization was not affected by experimental diets. An interaction effect was observed for blood high-density lipoprotein (HDL) concentration, which increased with both dietary inclusion of CHI and WS compared with the other diets, and CHI provided the lowest value of HDL cholesterol. Chitosan and whole raw soybean do not alter nutrient intake and total tract digestion; however, they decrease nitrogen urinary excretion and increase blood HDL cholesterol of heifers.

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Section: Discussionmentioning

confidence: 99%

Nutrient digestion, microbial protein synthesis, and blood metabolites of Jersey heifers fed chitosan and whole raw soybeans

et al. 2016

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“…Chitosan disrupts the barrier properties of the outer membrane of gram-negative bacteria (Helander et al 2001). It has been also reported that in dilute acid solutions, the positive charges of chitosan interferes with the negatively charged residues of macromolecules at the bacterial cell surface, presumably by competing with Ca + for electronegative sites on the membrane without conferring dimensional stability, rendering the membrane leaky (Begin and Calsteren 1999;Senel and McClure 2004). However, little is known regarding the antimicrobial activity of chitin derivatives (Je and Kim 2006).…”

Section: Discussionmentioning

confidence: 99%

Effects of Chitosan-oligosaccharide on diarrhoea in Hanwoo calves

Alam¹,

Kim²,

Kim³

et al. 2012

Vet. Med.

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ABSTRACT:The therapeutic effect of Chitosan-oligosaccharide (COS) on calf diarrhoea was investigated in Korean native (Hanwoo) calves (n = 86). The calves were divided into two groups; the untreated/control (n = 40) having routine diet only and the treated group (n = 46) receiving COS (50 ml/day) in addition to the routine diet for five days. Blood samples from each animal were collected before and after five days treatment and were subjected to complete blood count (CBC), chemistry screening (CS) and plasma protein assay (PPA). The in vitro efficacy of COS against the most common causal agents of calf diarrhoea (Escherichia coli and Salmonella typhimurium) was evaluated. Among the CBC parameters a significantly higher total red blood cell count (tRBC), haemoglobin (Hb) and packed cell volume (PCV) were noticed in the untreated group. In the CS parameters higher levels of blood urea nitrogen (BUN), creatinine and total bilirubin (TBL), and lower glucose were found in the untreated group. Significantly higher levels of albumin and α-globulin along with lower γ-globulin were noted in the untreated group. Among the treated group 41 (out of 46) calves recovered completely from diarrhoea and in the untreated group five calves (out of 40) spontaneously recovered after five days, revealing that the COS has a good therapeutic effect on diarrhoea in calves. The COS was found to effectively inhibit bacterial growth and pathogenicity up to 1:64 and 1:256 dilutions in the case of S. typhimurium and E. coli respectively. The results of this study revealed that the levels of different parameters of CBC (tRBC, Hb, PCV), CS (BUN, creatinine, TBL) and PPA (albumin, α-globulin, γ-globulin) profiles can reflect the severity of diarrhoea and dehydration and that COS can be successfully used for the clinical management of diarrhoea in Hanwoo calves.

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“…Colour, transparency, average weight, thickness, drug content and surface pH of the prepared CPC-chitosan buccal films are shown in table 4. Film thickness measurements varied with the van-der-Waals molecular volume counter ion of the casting solvent as films casted from LA (0.1-0.16 mm) were thicker than other films [31].…”

Section: Fig 1: Dsc Thermo Gram Of (A) Cpc (B) Chitosan and Films mentioning

confidence: 98%

“…The molecular volume of acetate ion represents barely the maximum space that the counter ion can be occupied within chitosan structure without interfering with the crystallite formed. Since the film strength depends on the number and the force of crystallites created during the formation of the film, it could be assumed that anions having a molecular volume superior to molecular volume of acetate anion will interfere with crystallite formation leading to a decrease in film strength [31]. In turn, % elongation of films casted from LA was higher than those casted from AA.…”

Section: Determination Of Mechanical Propertiesmentioning

confidence: 99%

Chitosan Mucoadhesive Buccal Films: Effect of Different Casting Solvents on Their Physicochemical Properties

Abouhussein

El-Bary

Shalaby

et al. 2016

Int J Pharm Pharm Sci

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Objective: The aim of this work is to investigate the influence of different casting solvents on the physicochemical properties of cetylpyridinium chloride (CPC) chitosan mucoadhesive buccal films.Methods: Screening formulations were prepared by casting solvent technique using organic acids; 1% acetic acid (AA), 1% lactic acid (LA) and inorganic acid; 0.1N HCl (FS1-FS3). Then, 2P 1 P X3P 1 P factorial design study was done using 2 factors; solvent type (AA, LA, Mixture of 0.1N HCl and LA) and solvent concentration (AA and LA; 1%, 2% and mixture of 0.1N HCl: 1% LA; 2:1, 1:2). Films were evaluated for their physicochemical properties through, mechanical properties, mucoadhesion, in vitro release of CPC and antimicrobial activity. Results:The studied factors showed a significant effect on both mucoadhesion and tensile strength. Film casted from 0.1 N HCl was brittle and did not show any elasticity, so it was used in further studies mixed with LA to improve physicochemical properties of the prepared films. Films casted from LA showed swelling for an initial period of 15 min then no more swelling occurred while swelling of those casted from AA occurred throughout approximately 2 h. A film containing 2:1 HCl: LA (F5) dissolved in both media while 1:2 HCl: LA (F6) showed swelling properties. This was reflected on the in vitro release of CPC in which F5 gave higher % released (DER 300 min R54.37%) than the other formulations. Conclusion:Casting solvent was proved to have a significant effect on the physicochemical properties of chitosan CPC mucoadhesive films.

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Antimicrobial films produced from chitosan

Cited by 200 publications

References 15 publications

Nutrient digestion, microbial protein synthesis, and blood metabolites of Jersey heifers fed chitosan and whole raw soybeans

Nutrient digestion, microbial protein synthesis, and blood metabolites of Jersey heifers fed chitosan and whole raw soybeans

Effects of Chitosan-oligosaccharide on diarrhoea in Hanwoo calves

Chitosan Mucoadhesive Buccal Films: Effect of Different Casting Solvents on Their Physicochemical Properties

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