Antimicrobial activities of blinding tree, Excoecaria agallocha against selected bacterial pathogens

Abstract

In aquaculture, the occurrence of bacterial resistance to synthetic antibiotics has become a serious problem. Therefore, research has been focused on finding new antimicrobial antibiotics from natural products to replace synthetic antibiotics. The aim of this study was to investigate the antimicrobial properties of mangrove plant blinding tree Excoecaria agallocha against selected fish pathogens namely Flavobacterium indicum, Chryseobacterium indologenes, Chryseobacterium gleum and Elizabethkingia meningoseptica previously named Flavobacterium meningosepticum. Mangrove leaves were obtained via extraction with 100 ml of methanol. The antimicrobial susceptibility test showed that the bacteria were resistant to Nitrofurantion, Gentamycin and Neomycin, and were sensitive to Flumequine. The minimum inhibitory concentration (MIC) of E. agallocha was 3.12 mg/ml, and minimum bactericidal concentration (MBC) was 6.25 mg/ml. Inhibition zones were significantly different (p< 0.05) depending on concentrations (100, 300 and 500 mg/ml) of the crude extraction of E. agallocha. The highest activity with LC 50 of E. agallocha was 94.19 (mg/ml). Methanolic extract of E. agallocha exhibited strong antimicrobial activity against these bacteria.

Phytochemical Composition and In vitro Antimicrobial, Antioxidant Activites of Methanolic Leaf Extracts From Excoecaria agallocha

“…On the other hand, the result of this work indicates that the significant differences within fractions may be attributed to the mangrove-plant extracts and their antimicrobial activity against pathogenic bacterial strains. Our observations in this study in accordance to the results of previous studies by 16,17,27,28 indicated that E. agallocha leaf extracts displayed antimicrobial activity.…”

“…The plant was identified at the Plant Taxonomy Laboratory, University Malaysia Terengganu (UMT). The extract of E. agallocha was prepared by standardized procedure as described by 17 .…”

AKTIVITAS ANTIBAKTERI PENYEBAB VIBRIOSIS TERHADAP UDANG WINDU DARI EKSTRAK HERBAL MANGROVE Sonneratia alba DAN Bruguiera gymnorrhiza

“…Selain itu, jenis mangrove ini dapat dijadikan sebagai alternatif pengobatan penyakit vibriosis pada larva lobster (Baskaran & Mohan, 2012). Sementara, Laith et al (2011); Laith & Najiah (2014) melaporkan bahwa E. agallocha efektif terhadap bakteri Flavobacterium spp penyebab penyakit bakterial pada ikan. Selanjutnya Saptiani et al (2012) melaporkan bahwa fraksi butanol dari daun Acanthus ilicifolius menghambat pertumbuhan V. harveyi lebih baik dibanding ekstrak etilasetat, sedangkan Ramesh et al (2014) (Sreenivasa et al, 2015).…”

A complete profile on blind-your-eye mangrove Excoecaria agallocha L. (Euphorbiaceae): Ethnobotany, phytochemistry, and pharmacological aspects

“…Thus, E. agallocha methanolic leaves extract showed high antimicrobial activity against these fish pathogens. [60] Eighty percent methanolic extract of wild grown plants, leaf derived callus, and in vitro raised plant leaves of E. agallocha were prepared and evaluated for antibacterial activity against Bacillus cereus , Bacillus subtilis , Klebsiella pneumoniae , Proteus vulgaris , S. aureus , Salmonella typhi . The result revealed that methanolic extract of leaf derived callus showed more antibacterial activity than the wild grown and in vitro raised plants.…”