Two isolates of Elizabethkingia meningoseptica were successfully isolated from kidney and skin tissue of diseased African Catfish (Clarias gariepinus) in Malaysia. The percentage similarity of both physical and biochemical characteristics for the isolates from kidney (K1) and skin samples (S1) as determined by BBL-Crystal and API 20E were 99 % and 95.4%, respectively. Furthermore, both isolates were identified via 16S rRNA gene sequences and showed more than 97% homology to sequences deposited in GenBank. The API ZYM results were analogous for both strains, with only minor quantitative variations. However, the isolates from kidney sample (K1) showed higher levels of enzymatic activity reaction towards esterase lipase and leucine arylamidase. Moreover, the enzymatic activity of α-galactosidase was detected at low level in kidney isolate and absence in skin isolate.
This study investigated the phytochemical, antimicrobial and antioxidant properties of methanolic leaf fractionated extracts of Excoecaria agallocha. Antibacterial activity test of fractionated extract of Excoecaria agallocha was conducted via Disk diffusion assay. Micro titer broth dilution technique was performed to determine the minimum inhibitory concentration (MIC) and Minimum Bactericidal Concentration (MBC). Qualitative and quantitative DPPH radical scavenging activity determines the antioxidant activity in E. agallocha. Chromatographic separation was completed using thin layer chromatography (TLC) and column chromatographic techniques. Phytochemical screening of fractionated extract revealed active constituents including alkaloids, saponins, flavonoids, terpenoid and tannins. In vitro antibacterial activity of columnfractionated extract demonstrated inhibitory effect against Flavobacterium indicum, Chryseobacterium meningosepticum, Chryseobacterium indologenes and Chryseobacterium gleum isolated from diseased fish. MIC and MBC of E. agallocha fractionated extract was 1.56 mg/ml and 3.12 mg/ml, respectively. TLC-based qualitative DPPH analysis revealed antioxidant activity in E. agallocha extract, specified by yellowish stain on maroon color TLC plate. These bands indicate the presence of various compounds that could act as potential antimicrobial agents. The present study indicates that E. agallocha leaves contain active phytochemicals which inhibit bacteria and could be useful in the treatment of microbial infections in fish.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.