Antibiotic resistance phenotypes and virulence-associated genes in Escherichia coli isolated from animals and animal food products in Tunisia

Badi, Souhir; Cremonesi, Paola; Abbassi, Mohamed Salah; Ibrahim, Chourouk; Snoussi, Mejdi; Bignoli, Giulia; Luini, M.; Castiglioni, Bianca; Hassen, Abdennaceur

doi:10.1093/femsle/fny088

Cited by 24 publications

(15 citation statements)

References 49 publications

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“…The E. coli resistance to trimethoprim-sulfamethoxazole in this study were 21.1%, that was similar to the research conducted by Badi et al (2018) which showed that E. coli resistance to trimethoprim-sulfamethoxazole were 36.9%. The second-highest resistance in this study occurred to ampicillin (26.3%), contrary to the study conducted by Messele et al (2017) that found high occurance (71.4%) of the E. coli resistance to oxytetracycline (5.3%) and ciprofloxacin (21.1%) in this study was lower than the study conducted by Rahman et al (2017) which reported the resistance in oxytetracycline (71.43%) but not resistant to ciprofloxacin (100% sensitive).…”

Section: Discussionsupporting

confidence: 89%

“…The resistance to cefotaxime in this study (21.1%) was higher compared to the study conducted by Badi et al (2018) which found a 7.7% of resistance in cefotaxime. The resistance in cefotaxime was considered as an early warning because cefotaxime was one of the third generations of cephalosporin that was known as drugs of choice in bacteremia caused by Gram-negative bacteria in humans (Park, 2014).…”

Section: Discussioncontrasting

confidence: 79%

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Antibiotic Resistance in Escherichia coli Isolated from Döner Kebab Sold in Dramaga Bogor, Indonesia

Sari¹,

Pisestyani²,

Lukman³

2020

Adv. Anim. Vet. Sci.

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This study was aimed to determine the occurrence of antibiotic resistance in Escherichia coli (E. coli) isolated from Döner kebab sold in Dramaga Bogor, West Java Province, Indonesia. A total of 43 samples of Dὂner kebab meat were taken from all of the vendors in the radius of 2 km around the border of IPB University campus, Dramaga Bogor. Isolation and identification of E. coli were referred to the Guideline for Laboratory Analysis on Examination of Microbial Contamination in Meat, Egg, and Milk in SNI 2897:2008 issued by the National Standardization Agency of Indonesia. The resistance against nine antibiotics were tested using Kirby-Bauer disk diffusion method based on the standard of Clinical Laboratory Standards Institute (CLSI) in 2018. The result showed that nine samples were positive containing E. coli (20.9%) and 84.2% of E. coli isolates were resistant to eight antibiotics: gentamicin (57.9%), ampicillin (26.3%), cefotaxime, trimethoprim-sulfamethoxazole, and ciprofloxacin (21.1%), amoxicillin-clavulanate (10.5%), enrofloxacin, and oxytetracycline (5.3%). All of E. coli isolates were susceptible to chloramphenicol (100%). There were 21.1% of isolates also resistant to three or more classes of antibiotics that were known as multi-drug resistant (MDR) with different resistant patterns.The presence of E. coli resistant antibiotic in kebab meat could results serious problems in human health.

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Section: Discussionsupporting

confidence: 89%

Section: Discussioncontrasting

confidence: 79%

Antibiotic Resistance in Escherichia coli Isolated from Döner Kebab Sold in Dramaga Bogor, Indonesia

Sari¹,

Pisestyani²,

Lukman³

2020

Adv. Anim. Vet. Sci.

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“…The EPEC-a strains have been detected in birds reared in the conventional system and its products (Dutta et al 2011, Alonso et al 2012, Samanta et al 2015, Doregiraee et al 2016, Badi et al 2018, as occurred in the present study.…”

Section: Epec and Stec Characterizationsupporting

confidence: 73%

“…The Enteropathogenic Escherichia coli (EPEC) and Shigatoxigenic E. coli (STEC) strains are pathogens of public health importance due to their ability to cause enteric diseases in humans (Ifeanyi et al 2016, Fierz et al 2017, Torres 2017) these pathogens are isolated from poultry and poultry products (Dutta et al 2011, Alonso et al 2012, Samanta et al 2015, Doregiraee et al 2016, Badi et al 2018.…”

Section: Introductionmentioning

confidence: 99%

Detection of the mcr-1 gene in Enteropathogenic Escherichia coli (EPEC) and Shigatoxigenic E. coli (STEC) strains isolated from broilers

et al. 2020

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Enteropathogenic Escherichia coli (EPEC) and Shigatoxigenic E. coli (STEC) strains are among the major pathotypes found in poultry and their products, which are capable of causing human enteric infections. Colistin has been claimed the drug of choice against diseases caused by multidrug-resistant Gram-negative bacteria (MDRGN) in humans. The mcr-1 gene was the first plasmidial gene that has been described to be responsible for colistin resistance and has also been detected in birds and poultry products. Our study aimed to detect the mcr-1 gene in enteropathogenic strains of E. coli in order to evaluate the resistance to colistin in broilers. The material was obtained from 240 cloacal samples and 60 broiler carcasses. The strains were isolated by the conventional bacteriological method and by the virulence genes, which characterize the enteropathogenic strains and resistance, and the samples were detected by polymerase chain reaction (PCR). Of the 213 isolated strains of E. coli, 57 (26.76%) were characterized as atypical EPEC and 35 (16.43%) as STEC. The mcr-1 gene was found in 3.5% (2/57) of the EPEC strains and 5.7% (2/35) of the STEC strains. In this study, it was possible to confirm that the mcr-1 resistance gene is already circulating in the broiler flocks studied and may be associated with the pathogenic strains.

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“…Foods of animal origin are considered serious sources of food-borne diseases worldwide. Moreover, the situation worsens in developing countries due to difficulties in achieving optimum hygienic food handling practices (Schlundt et al, 2004;Newell et al, 2010;Badi et al, 2018). Foodborne pathogens cause high economic losses in developing countries due to medical care and social costs (Fratmico et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Molecular Determinants of Multiple Antibiotic Resistant E. coli Isolated from Food of Animal Origin

Seedy¹,

Samy²,

Khairy³

et al. 2020

Adv. Anim. Vet. Sci.

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Multiple antibiotic-resistant E. coli usually contaminates foods of animal origin representing a major threat to public health. The purpose of this study was to demonstrate multiple antibiotic-resistant E. coli as a serious food contaminating bacteria in milk, meat and their products. One hundred and twenty five (125) samples were collected from meat, milk and their products from different markets in Beni-Suef and Giza Governorates, Egypt. The isolated E. coli were subjected to antimicrobial sensitivity testing against ampicillin, tetracycline and streptomycin and resistance genes were detected using polymerase chain reaction (PCR). Out of 125 samples, 41 E. coli were isolated. Serogrouping of E. coli isolates revealed O157, O44, O119, O168, O6, O158, O164, O126 and O125 serogroups. Most of E. coli isolates were multidrug resistant (MDR). E. coli isolates were resistant to ampicillin, amoxicillin, kanamycin, tetracycline and streptomycin with percentages of 63.4, 61, 53.7, 41.5 and 14.6%, respectively. Out of tetracycline resistant isolates (n=17), 14 isolates harbored tetB gene (82.4%), while six isolates harbored tetA gene (35.3%). Meanwhile, both tetA and tetB were harbored in 4 isolates with a percentage of 23.5% Ampicillin resistance gene bla TEM found in 76.5% of the ampicillin resistant isolates. Streptomycin resistant genes aadA1 and aadA2 were represented in 50% and 0% of the isolates, respectively. Foods of animal origin is considered an important source of multiple antibiotic resistant E. coli that can be a major source of food-borne diseases.

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Antibiotic resistance phenotypes and virulence-associated genes in Escherichia coli isolated from animals and animal food products in Tunisia

Cited by 24 publications

References 49 publications

Antibiotic Resistance in Escherichia coli Isolated from Döner Kebab Sold in Dramaga Bogor, Indonesia

Antibiotic Resistance in Escherichia coli Isolated from Döner Kebab Sold in Dramaga Bogor, Indonesia

Detection of the mcr-1 gene in Enteropathogenic Escherichia coli (EPEC) and Shigatoxigenic E. coli (STEC) strains isolated from broilers

Molecular Determinants of Multiple Antibiotic Resistant E. coli Isolated from Food of Animal Origin

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