Aim:The aim of our study was polymerase chain reaction (PCR) detection of the genes responsible for the multiple antibiotic resistance S. aureus isolated from food of animal origin in Egypt.Materials and Methods:A total of 125 samples were randomly collected from milk, meat, and their products from Giza and Beni-Suef Governorates markets. The S. aureus isolates were subjected to antimicrobial sensitivity tests using four antibacterial disks (Oxoid), and then the polymerase chain reaction (PCR) was performed for detection of antibiotic resistance genes.Results:Out of 125 samples, 19 S. aureus isolates were detected. All detected isolates were multiple drug resistance (MDR). The penicillin-, erythromycin-, kanamycin-, and tetracycline-resistant isolates were examined by PCR for resistance genes blaZ, (msrA, ermB, and ermC), aac(6’)aph (2”), and tetK. The isolates harbored these resistance genes with percentage of 100% (100%, 0%, and 100%), 62.5%, and 100%, respectively.Conclusion:Contaminated foods of animal origin may represent a source of MDR S. aureus that can be a major threat to public health.

ARTICLE INFOForty eight clinically diseased broiler chickens from different (n=18) farms from Beni Suef and El-Fayoum Governorates were subjected to euthanasia and post mortem examination. Lesions include airsaculitis, pericarditis and perihepatitis. Bacteriological examination showed that 22.9% of isolates were E. coli positive. Serogrouping of isolates revealed O125, O112, O91, O157, O115 and O25 % of each serogroups with an incidence rate of 18. 2%, 9.1%, 9.1%, 9.1%, 9.1%, and 9.1%, respectively and four strains were untyped. Antimicrobial sensitivity tests against 17 antimicrobials showed that the most common resistance patterns were against penicillin, lincomycin, oxytetracycline, clindamycin, amoxycillin and erythromycin followed by nalidix acid and trimethoprim. On the other hand, the most potent antimicrobials were colistin sulphate, gentamycin, doxycycline and ceftriaxone followed by enrofloxacin, ciprofloxacin, norfloxacin, chloramphenicol and lastly ampicillin. PCR showed that all isolates had Blactam resistant gene (blaTEM) and tetracycline resistance gene A (tetA) but only 18 % have quinolones resistance gene A (qnrA).

The experiment was designed to investigate the immunomodulating effect of lector 50 on general health and immune response of broiler chicks to Mycoplasma gallisepticum vaccination in commercial broiler chicks. the obtained results reveled significantly higher effects on body weight ,bursal, and thymic index on lector treated group of chickens, while no effects on spleen index. Also significant improvement in total and differential leukocytic count as well as significantly higher antibody titer was detected by ELISA in lector 50 treated groups.

Food contaminated with multiple antibiotic-resistant S.aureus can be a major threat to the public health. The purpose of this study was to isolate S.aureus from different food sources, determine their antimicrobial susceptibility as well as detection of mecA gene among some resistant isolates. Out of 125 samples, 19 S.aureus isolates were isolated, and the antimicrobial susceptibility testing showed high resistance against kanamycin, penicillin G, oxacillin, erythromycin and tetracycline were the most resistant antimicrobials agents. All the tested isolates isolates were multiple drug resistant (MDR).Eight out of 19 isolates were phenotypically resistant to oxacillin as well as they were carriers for mecA gene.

Multiple antibiotic-resistant E. coli usually contaminates foods of animal origin representing a major threat to public health. The purpose of this study was to demonstrate multiple antibiotic-resistant E. coli as a serious food contaminating bacteria in milk, meat and their products. One hundred and twenty five (125) samples were collected from meat, milk and their products from different markets in Beni-Suef and Giza Governorates, Egypt. The isolated E. coli were subjected to antimicrobial sensitivity testing against ampicillin, tetracycline and streptomycin and resistance genes were detected using polymerase chain reaction (PCR). Out of 125 samples, 41 E. coli were isolated. Serogrouping of E. coli isolates revealed O157, O44, O119, O168, O6, O158, O164, O126 and O125 serogroups. Most of E. coli isolates were multidrug resistant (MDR). E. coli isolates were resistant to ampicillin, amoxicillin, kanamycin, tetracycline and streptomycin with percentages of 63.4, 61, 53.7, 41.5 and 14.6%, respectively. Out of tetracycline resistant isolates (n=17), 14 isolates harbored tetB gene (82.4%), while six isolates harbored tetA gene (35.3%). Meanwhile, both tetA and tetB were harbored in 4 isolates with a percentage of 23.5% Ampicillin resistance gene bla TEM found in 76.5% of the ampicillin resistant isolates. Streptomycin resistant genes aadA1 and aadA2 were represented in 50% and 0% of the isolates, respectively. Foods of animal origin is considered an important source of multiple antibiotic resistant E. coli that can be a major source of food-borne diseases.