Analysis of CCND1 protein and circulatory antioxidant enzyme activity association in oral squamous cell carcinoma

Abstract

Antioxidants are involved in the process of cellular damage prevention, which is considered as an avenue for cancer development. Free radicals are produced in the body upon exposure to stress, cigarette smoke, alcohol, toxins found in personal care products, pesticides in foods, radiation from the sun, viruses, germs or fungi etc. CCND1/CyclinD1 protein was found to be overexpressed in Oral squamous cell carcinoma. One hundred patients with oral squamous cell carcinoma were recruited along with hundred controls for this study from MNJ institute of Oncology with the approval of Ethics Committee, 5 ml blood samples were collected from each patient and centrifuged to collect serum for various assays. The antioxidant enzymes like catalase, SOD, GPX and GST were estimated using enzymatic assays. Results were expressed as unit of activity for mg of protein. Insilco analysis is performed using STRING v 11 Protein interaction tool. The patients with oral cancer had significantly reduced activities of SOD, GST and GPX (1.49 ± 0.49, 3.97 ± 0.86 and 10.7 ± 0.73 respectively) compared to healthy controls (4.37 ± 1.43, 6.10 ± 1.12 and 13.8 ± 1.25 respectively) (p < 0.005). However no significant difference was observed with regard to catalase activity (2.71 ± 6.51 and 4.03 ± 1.48) (p = 0.28). The proteins interaction PPI enrichment p-value was found to be 3.22e-10 predicted significantly more interactions. Our research findings shown that there was a decline in activity of superoxide dismutase, glutathione peroxidase and glutathione s transferase in addition, personal habits like smoking play a major role in the development and progression of oral carcinogenesis and based on Insilco analysis results CCND1/Cyclin D1 could be the potential therapeutic target in oral squamous cell carcinoma.

Assessment of Antioxidant Enzyme Superoxide Dismutase (SOD) in Oral Cancer: Systematic Review and Meta-Analysis

“…The selected studies were conducted in several nations, including IndiaClick or tap here to enter text [16-22, 24, 26-28, 30-34, 37-45, 47, 49-51, 53], Italy [23], Australia [25], Pakistan [35,48], China [36], Poland [29,52], and Saudi Arabia [46,54].…”

“…Rasheed et al[25] Patel et al[26] Raghavendra et al[27] Gokul et al[28] Giebułtowicz et al[29] Srivastava K et al[30] Gurudath et al[31] Shilpasree et al[32] Shetty et al[33] Bagul[34] Rasool et al[35] Huo et al[36] Shetty et al[37] Manasavena et al[38] Singh et al[39] Ganjre and Bagul[40] Rai et al[41] Thomas and Sethupathy[42] Shankarram et al[43] Mishra et al[44] Nyamathi et al[45] Srivastava K et al[46] Banerjee et al[47] Khan et al[48] Sadaksharam[49] Subash et al[50] Basu and Guhan[51] Babiuch et al[52] Shahi et al[53] Sushma et al[54] …”

Assessment of glutathione peroxidase enzyme response and total antioxidant status in oral cancer – Systematic review and meta‐analysis

“… 21 , 22 , 23 , 24 , 27 , 28 , 32 , 33 , 36 , 38 , 41 , 47 Out of the four included studies that analyzed the GPx activity in blood samples except one, 48 the remaining studies displayed diminished enzyme activity in the OSCC group compared to healthy controls ( p < .05). 26 , 30 , 35 In consideration of serum samples, three studies displayed reduced activity, 25 , 37 , 51 and the remaining two depicted enhanced GPx activity in the cancerous group compared to the normal control group ( p < .05). 34 , 45 When considering GPx values in salivary samples, two studies displayed intensified activity ( p < .05) 39 , 49 ; however, one research depicted an insignificant reduction of the GPx activity.…”

Network Pharmacology Identifies Therapeutic Targets and the Mechanisms of Glutathione Action in Ferroptosis Occurring in Oral Cancer

“…As a key enzyme regulating ferroptosis, GPx4 can inhibit the occurrence of ferroptosis by catalyzing the reduction of lipid peroxides ( Xuan et al, 2021 ). Patients with OC have markedly low levels of superoxide dismutase, GSH peroxidase, and GSH transferase ( Sushma et al, 2021 ). An in vitro study demonstrated that enhanced intracellular GSH activity induced by natural compounds might have anti-oral cancer action by modulating oxidative stress, autophagy, and cell death ( Contant et al, 2021 ).…”