An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Lush

Savita, .; Singh, B. N.; Virk, G. S.; Nagpal, Avinash Kaur

doi:10.1007/s12298-011-0055-9

Cited by 31 publications

(20 citation statements)

References 28 publications

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“…In BAP (1.0 mg/L) + NAA or 2,4-D with increasing concentrations (0.5 -1.0 mg/L), shown a decreases in the plant regeneration as well of number of shoot produced. A similar response was reported [10] on shoot regeneration of citrus callus a member of Rutaceae family. The in vitro rooted plantlets transplanted into plastic pots in 75% shading net house were exhibited 98% survival rate after 60 days of transplanting (Figures 1(h)-(i)).…”

Section: Plant Regenerationsupporting

confidence: 83%

Direct Shoot Regeneration from Callus of Melicope lunu-ankenda

Rahman¹,

Othman²,

Kamaruddin³

et al. 2015

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Melicope lunu-ankenda is commonly used in traditional medicine. The conventional propagation method for this species is inefficient due to low propagation rate and its lengthy period to maturity. In addition, insufficient planting materials often pose a problem for the plantation sector. The tissue culture technique is best alternative to overcome the problems. The callus induction and direct shoot regeneration protocols for M. lunu-ankenda were established. Callus was successfully initiated from leaves explants cultured in MS medium added with 2,4-D at concentrations 0.5 to 5.0 mg/L singly or in combination with NAA at concentrations 1.0 to 10 mg/L. Shoot was regenerated from callus in phytohormone-free medium, BAP at concentrations 0.5 -5.0 mg/L singly or in combination of BAP with NAA or 2,4-D at concentration 0.5 and 1.0 mg/L, respectively. BAP at 1.0 mg/L induced the highest shoot regeneration rate (80%) and number of plantlet per calli. The established methods might be used for production of phytochemicals and plantlets in large scale.

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Section: Plant Regenerationsupporting

confidence: 83%

Direct Shoot Regeneration from Callus of Melicope lunu-ankenda

Rahman¹,

Othman²,

Kamaruddin³

et al. 2015

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“…Highest callus induction frequency was observed in MSCIM and N6CIM media with supplementation of 4.0 mg/l NAA and relatively lower frequency was found in B5CIM media at the same concentration. Combination of 2,4-D and NAA showed relatively similar results to that of 2,4-D supplemented media [23].…”

Section: Resultsmentioning

confidence: 52%

In-Vitro Regeneration of Citrus sinensis (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

Hasan¹,

Hasan²,

Foysal³

et al. 2019

AJPS

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In-vitro callus induction and regeneration method was developed using different plant growth regulators (PGRs), and basal media (Murashige and Skoog (MS), CHU (N6) and Gamborg (B5) media) of Citrus sinensis (L.) Osbeck. Observations of the effect of PGRs were carried out using different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D),1-naphthalene acetic acid (NAA) and combinations of 2,4-D and NAA using different basal media. This study found Citrus sinensis (L.) Osbeck exhibited a high frequency of callus induction on MS medium supplemented with 3 mg/L 2,4-D and callus induction frequency was 86.7% ± 3.4% whereas N6 and B5 showed lower callus induction frequency of 83.3% ± 8.8% and 82.2% ± 1.9% respectively compared to that of MS media with supplementation of the same hormone. Among the induced calli, the morphological analysis showed only 40% -50% was embryogenic calli. Regeneration of plantlets from calli was done using different concentrations and combinations of auxin and cytokinin. The study showed that 3 mg/L 6-benzylaminopurine (BAP) supplemented medium has the maximum potential to promote regeneration of Citrus sinensis (L.) Osbeck from embryogenic calli with the frequency of 89.3% ± 8.8% but no regeneration occurred from the non-embryogenic calli. The regenerated plantlets were rooted on MS medium with supplementation of 5 mg/l NAA.These observations in Citrus sinensis (L.) Osbeck regeneration will be helpful for genetic improvement with desired traits.

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“…Very less work is done on this species specially in Bangladesh and that's why a standard protocol for micropropagation is necessary to generate more disease-free plants of this species. Seed is an excellent source of explant to produce the fungus resistant plant because pathogen can't reach to the seed level even after severe infection to the plant [25]. Although regeneration in Citrus sp.…”

Section: Resultsmentioning

confidence: 99%

An Efficient Regeneration System for Native Orange (Citrus reticulata) through In-Vitro Culture Technique

Khan¹,

Hoque²,

Islam³

et al. 2019

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Citrus reticulata (Mandarin Orange), commonly known as "Sweet Orange", is one of the most difficult plants to improve through traditional breeding approaches as it poses various biological limitations that greatly hinder the cultivar improvement. In the present study, using the fresh seed of native orange as explant, an efficient, reproducible, regeneration method was developed through in vitro organogenesis. Mature, healthy and dehusked seeds were treated with Murashige and Skoog, (MS) media containing 3% sucrose, 0.7% agar supplemented with different concentrations and combinations of phytohormones. The highest calli initiation (93.3% ± 0.5%) responses were observed on MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) at 3.0 mg/L followed by 2,4-D at 3.5 mg/L (86.7% ± 1.75%) in this experiment. Maximum shoot regeneration (86.7% ± 3.35%) responses were reported using MS medium supplemented with the combination of 6-benzylaminopurine (BAP) at 3.0 mg/L and 1-naphthaleneacetic acid (NAA) at 2.0 mg/L. MS medium supplemented with NAA at 1.0 mg/L showed the best rooting (80% ± 2.89%) response in comparison to (70% ± 5.20%) indole-3-butyric acid (IBA) at 1.0 mg/L. The regenerated plantlets were acclimatized in pots containing sterile garden soil mixture to examine their response in natural conditions.

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An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Lush

Cited by 31 publications

References 28 publications

Direct Shoot Regeneration from Callus of <i>Melicope lunu-ankenda</i>

Direct Shoot Regeneration from Callus of <i>Melicope lunu-ankenda</i>

<i>In-Vitro</i> Regeneration of <i>Citrus sinensis</i> (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

An Efficient Regeneration System for Native Orange (<i>Citrus reticulata</i>) through <i>In-Vitro</i> Culture Technique

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An efficient plant regeneration protocol from callus cultures of Citrus jambhiri Lush

Cited by 31 publications

References 28 publications

Direct Shoot Regeneration from Callus of &lt;i&gt;Melicope lunu-ankenda&lt;/i&gt;

Direct Shoot Regeneration from Callus of &lt;i&gt;Melicope lunu-ankenda&lt;/i&gt;

&lt;i&gt;In-Vitro&lt;/i&gt; Regeneration of &lt;i&gt;Citrus sinensis&lt;/i&gt; (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

An Efficient Regeneration System for Native Orange (&lt;i&gt;Citrus reticulata&lt;/i&gt;) through &lt;i&gt;In-Vitro&lt;/i&gt; Culture Technique

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About

Direct Shoot Regeneration from Callus of <i>Melicope lunu-ankenda</i>

Direct Shoot Regeneration from Callus of <i>Melicope lunu-ankenda</i>

<i>In-Vitro</i> Regeneration of <i>Citrus sinensis</i> (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

An Efficient Regeneration System for Native Orange (<i>Citrus reticulata</i>) through <i>In-Vitro</i> Culture Technique