&amp;lt;i&amp;gt;In-Vitro&amp;lt;/i&amp;gt; Regeneration of &amp;lt;i&amp;gt;Citrus sinensis&amp;lt;/i&amp;gt; (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

Hasan, Md. Nazmul; Hasan, Md. Mahmud; Foysal, Shakhawat Hossain; Hoque, Hammadul; Khan, Md. Fahim; Bhuiyan, Md. Fahmid Hossain; Prodhan, Shamsul H.

doi:10.4236/ajps.2019.102022

Cited by 12 publications

(10 citation statements)

References 18 publications

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“…After completion of adding the components, media was melted in oven and 10 mL media poured into each test tube (2.5×8 cm) and sealed with cotton made cork. The media was sterilized at 121°C temperature, 15 psi pressure for 20 min (Hasan et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

In Vitro Callus Induction and Regeneration of Medicinal Plant Datura innoxia

Twaij¹,

Taha²,

Hasan³

2019

American Journal of Biochemistry and Biotechnology

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The present study indicate the possibility of the development of callus from stem cut, leaf and root explants and regeneration of Datura plant (Datura innoxia) on Murashige and Skoog (MS) media supplemented with different concentrations of 6-Benzylaminopurine (BA) and 1-Naphthaleneacetic (NAA). Sterilized explants were inoculated and incubated in culture shade under different conditions (light and dark). Data analysis showed that maximum percentage (83%) of calli were induced from stem cut explants on MS media supplemented with NAA and BA at 0.5 and 1.0 mg/l respectively under dark condition. Maximum fresh and dry weight was found 395.63 mg and 35.64 mg respectively with supplementation of 0.5 mg/l NAA and 1 mg/l BA. Stem cut derived calli were transfer to MS regeneration medium supplementing different concentrations of plant regulators (PGRs) under different conditions. Maximum regeneration (78%) was found on MS media supplementing 1 mg/l of BA and 1 mg/l off NAA under dark condition. The attempt for callus induction from the explants (stem, leaf, root) using MS media with supplementation of different combinations of 2, 4-Dichlorophenoxyacetic acid (2, 4-D), BA and NAA, Kinetin (kin) under both light and dark conditions were carried out but no significant results were found.

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Section: Methodsmentioning

confidence: 99%

In Vitro Callus Induction and Regeneration of Medicinal Plant Datura innoxia

Twaij¹,

Taha²,

Hasan³

2019

American Journal of Biochemistry and Biotechnology

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“…Inoculation process: as earlier said all the process till this to be done under septic and here in this step the desired explants are rooted into sterilized medium. 26…”

Section: Plant Tissue Culture Processmentioning

confidence: 99%

“…The media with explants are aseptically sealed and kept in a room for further development under diffused light 25±2 0 C having 50 to 60% relative humidity. 26…”

Section: Explants Developmentmentioning

confidence: 99%

“…As in artificial condition the humidity and other requirement are conditioned developed and that because sometimes these plants from explants are not therefore ready for coping up in field conditions and they are hardened to catch up with field environment. 26,27 These steps above are overall major steps to be followed during setting up a plant culture, but it finally begins depending on a genotype selected based on identifying a problem which is to solve as an outcome of this culture. For each specific type of genotype and target aim a particular protocol to be followed each time.…”

Section: Hardening Of Micro Plantsmentioning

confidence: 99%

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Trends in the Use of Tissue Culture, Applications and Future Aspects

Twaij

Jazar

Hasan

2020

IJPB

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Plant tissue culture has developed widely incorporated into biotechnology, the agricultural systems being a key factor to support many pharmaceutical and industrial outcomes. Since 1902 there is vast progress in plant culture and its application has emerged having great diversity in the science filed. Due to development and desire to grow on high scale production in the past few decades, tissue culture techniques were manipulated for improvement of plant growth, biological activities, transformation, and secondary metabolites production. A significant advance in techniques has been sought to deal with problems of low concentrations of secondary metabolites in whole plants. The augmented use of plant culture is due to a superior perceptive of plant oriented compounds and secondary metabolites from economically important plants. Due to development in modern techniques, several particular protocols have been developed for the production of a wide array of secondary metabolites of plants on a commercial scale. Plant tissue culture has to lead to significant contributions in recent times and today they constitute an indispensable tool in the advancement of agricultural sciences and modern agriculture. This review would enable us to have an analysis of plant tissue culture development for agriculture, human health and wellbeing in general.

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“…With the advancement of science, modern techniques like marker-assisted breeding, somaclonal variation, genetic engineering and CRISPR-Cas9 could improve rice to overcome these problems (Romero and Gatica-Arias, 2019). However, a suitable regeneration system of rice callus derived from mature seeds is a prerequisite for almost every modern technique (somaclonal variation, Agrobacterium-mediated transformation (Hasan et al, 2019a), CRISPR-Cas9 mediated genome editing) of rice improvement (Miyao et al, 2012;Hiei and Komari, 2008;Mazumdar et al, 2016). To date, the development of an effective and robust regeneration system of rice has been limited to only a handful of rice varieties and cultivars, while the mature seed-derived callus regeneration of many agro-economically important rice cultivars have not yet been studied (Paul and Roychoudhury, 2019).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Plant Growth Regulators (PGRs) on Efficient Regeneration of 12 Recalcitrant Indica Rice (Oryza Sativa L.) Genotypes

Hasan

Bhuiyan

Hoque

et al. 2021

American Journal of Biochemistry and Biotechnology

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Biotechnological approaches provide an efficient and expeditious system for developing agronomic features of rice at the cellular and molecular level compared to traditional breeding methods. The in-vitro regeneration method optimization of recalcitrant indica rice genotypes is a critical step for the biotechnological approaches for further development as the optimum concentrations of Plant Growth Regulators (PGRs) for in-vitro regeneration vary from genotype to genotype. Here, 12 important rice genotypes, including local cultivars and varieties, were collected from different regions of Bangladesh and evaluated for their in-vitro regeneration potentiality. Mature dehusked sterilized rice seeds were inoculated on different basal media (MS, N6, B5) supplemented with different concentrations of 2,4-Dichlorophenoxyaceitic acid (2,4-D) for callus induction. Induced calli were transferred to N6 media supplemented with different combinations of 6-Benzylaminopurine (BA) and 1-Napthaleneacetic acid (NAA) for shoot induction. Regenerated shoots were rooted on half-strength MS media without supplementation of any hormones. Treatments of different concentrations and combinations of plant growth regulators showed varying results. Maximum callus induction frequencies were observed with 2.5-3.0 mg/l supplementation of 2,4-D on N6 media where Saat, Jamaibabu and Nimikhanai showed the highest callus induction frequencies (84.44%; 78.89 and 80% respectively) and maximum shoot initiation was observed at 2.5 mg/l BA +1.0 mg/l NAA and 3.0 mg/l 6-BA +1.5 mg/l NAA supplemented media where rice genotypes BRRI Dhan71, Nayapaijam, Nimikhanai and BRRI Dhan28 showed the highest shoot induction frequencies-86.67, 85.00, 86.67 and 88.33% respectively. Nearly all of the induced shoots showed root formation on half-strength MS media. This study demonstrates variations in regeneration response of the rice genotypes where cultivars Jamaibabu, Nayapaijam and Nimikhanai showed the highest in-vitro regeneration potential.

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<i>In-Vitro</i> Regeneration of <i>Citrus sinensis</i> (L.) Osbeck from Mature Seed Derived Embryogenic Callus on Different Solid Basal Media

Cited by 12 publications

References 18 publications

In Vitro Callus Induction and Regeneration of Medicinal Plant Datura innoxia

In Vitro Callus Induction and Regeneration of Medicinal Plant Datura innoxia

Trends in the Use of Tissue Culture, Applications and Future Aspects

The Effect of Plant Growth Regulators (PGRs) on Efficient Regeneration of 12 Recalcitrant Indica Rice (Oryza Sativa L.) Genotypes

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