An Efficient Regeneration System for Native Orange (<i>Citrus reticulata</i>) through <i>In-Vitro</i> Culture Technique

Abstract

Citrus reticulata (Mandarin Orange), commonly known as "Sweet Orange", is one of the most difficult plants to improve through traditional breeding approaches as it poses various biological limitations that greatly hinder the cultivar improvement. In the present study, using the fresh seed of native orange as explant, an efficient, reproducible, regeneration method was developed through in vitro organogenesis. Mature, healthy and dehusked seeds were treated with Murashige and Skoog, (MS) media containing 3% sucrose, 0.7% agar supplemented with different concentrations and combinations of phytohormones. The highest calli initiation (93.3% ± 0.5%) responses were observed on MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) at 3.0 mg/L followed by 2,4-D at 3.5 mg/L (86.7% ± 1.75%) in this experiment. Maximum shoot regeneration (86.7% ± 3.35%) responses were reported using MS medium supplemented with the combination of 6-benzylaminopurine (BAP) at 3.0 mg/L and 1-naphthaleneacetic acid (NAA) at 2.0 mg/L. MS medium supplemented with NAA at 1.0 mg/L showed the best rooting (80% ± 2.89%) response in comparison to (70% ± 5.20%) indole-3-butyric acid (IBA) at 1.0 mg/L. The regenerated plantlets were acclimatized in pots containing sterile garden soil mixture to examine their response in natural conditions.

Evaluation of explants for in vitro propagation of Citrus indica Tanaka - An Endangered Species

“…Kamruzzaman et al (2015) observed 90% callus induction from leaf explants of C. reticulata on MS medium + 2, 4-D (1.0 mg/l). Khan et al (2019) observed highest calli initiation (86.7%) from leaf disc of Citrus reticulata on MS medium supplemented with 2, 4-D (3.0 mg/l).…”

Micropropagación in vitro de naranja agria (Citrus aurantium L.) a partir de segmentos nodales

“…Resultando en clones uniformes a partir de una planta madre libre de enfermedades y de gran producción, conservando y garantizando los rasgos seleccionados 14 . Además, la propagación in vitro permite obtener material vegetal durante todo el año, de esta manera no habría un limitante como el suministro de semilla o plantines de invernaderos 15,16 .…”