Amination of Polystyrene Microwells: Application to the Covalent Grafting of DNA Probes for Hybridization Assays

Zammatteo, Nathalie; Girardeaux, Christophe; Delforge, Dominique; Pireaux, Jean‐Jacques; Remacle, José

doi:10.1006/abio.1996.0135

Cited by 60 publications

(40 citation statements)

References 11 publications

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“…Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to characterize the assembled process of the multilayer films in 10 mM [Fe(CN) 6 ] 3À/4À and 0.1 M KCl at 100 mV s…”

Section: Film Assembling and Characterizationmentioning

confidence: 99%

“…There are many DNA hybridization analysis methods, such as radiochemical [6], surface plasmon resonance spectroscopy [7,8] and electrochemiluminescence [9] etc. Electrochemical techniques are well suited for rapid detection of specific DNA sequences for their high sensitivity, small dimensions, low cost, and compatibility with microfabrication technology of transducers [10 -12].…”

Section: Introductionmentioning

confidence: 99%

“…In this work, we selected (MWCNTs/GNPs) 7 films modified electrode to performed the DNA hybridization analyses. Figure 2B shows the impedance features of electrodes at different modification steps in the presence of [Fe(CN) 6 ] 3À/4À . As shown in Figure 2B, significant differences in the impedance spectra were observed during stepwise modification of the electrodes.…”

mentioning

confidence: 99%

“…Figure 2A shows typical cyclic voltammograms of [Fe(CN) 6 ] 3À/4À at different layer numbers. Obviously, from one to seven layers, both the anodic and cathodic peak current increased regularly with the number of layers increased (as shown in the inset of Fig.…”

mentioning

confidence: 99%

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A Novel Electrochemical DNA Biosensor Fabricated with Layer‐by‐Layer Covalent Attachment of Multiwalled Carbon Nanotubes and Gold Nanoparticles

Zhang

Pan

et al. 2008

Electroanalysis

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A novel DNA biosensor has been fabricated for the detection of DNA hybridization based on layer-by-layer (LBL) covalent assembly of gold nanoparticles (GNPs) and multiwalled carbon nanotubes (MWCNTs). The stepwise LBL assembly process was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The hybridization events were monitored by differential pulse voltammetry (DPV) measurement of the intercalated doxorubicin, and the factors influencing the performance of the DNA hybridization was investigated in detail. The signal was linearly changed with target DNA concentration increased from 0.5 to 0.01 nM, and had a detection limit of 7.5 pM (signal/noise ratio of 3). In addition, the DNA biosensor showed an excellent reproducibility and stability under the DNA-hybridization conditions.

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Section: Film Assembling and Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A Novel Electrochemical DNA Biosensor Fabricated with Layer‐by‐Layer Covalent Attachment of Multiwalled Carbon Nanotubes and Gold Nanoparticles

Zhang

Pan

et al. 2008

Electroanalysis

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“…A polystyrene surface can be modified to improve its hydrophilicity by incorporating various functional groups such as hydroxyl, amino, carbonyl, carboxyl, etc. on its surface [12][13][14]. In most hapten based ELISAs, hapten molecules are usually bound to polystyrene microtiter wells indirectly by coating the wells with protein-hapten conjugates, since direct attachment of hapten molecules to a polystyrene surface is not possible due to the lack of available functional groups on polystyrene.…”

Section: Introductionmentioning

confidence: 99%

Direct hapten coated immunoassay format for the detection of atrazine and 2,4-dichlorophenoxyacetic acid herbicides

Kaur

Boro

Wangoo

et al. 2008

Analytica Chimica Acta

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Stability of plasma-polymerized allylamine films with sterilization by autoclaving

Calderon

Harsch

Gross

et al. 1998

J. Biomed. Mater. Res.

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The stability of plasma-polymerized allylamine films with autoclaving sterilization cycles was investigated. Polymerized films were deposited under pulsed plasma conditions using two different duty cycles to provide surfaces having different initial amino group concentrations. The film properties were analyzed by XPS and water contact angle measurements before and after autoclaving. The reactions of these surfaces with trifluoroacetic anhydride provided quantitation of the amino surface concentrations before and after autoclaving. In general, the plasma-polymerized films exhibit good stability vis à vis the autoclaving process, with relatively high retention of the surface amino groups. The results of this work are of specific value with respect to tissue culture studies in which surface modifications involving the introduction of amino groups have been shown to have high efficacy in promoting cell growth. The results obtained suggest that the simple one-step plasma treatment process is a viable alternative to the more cumbersome surface modification procedures currently employed to introduce amino groups in these tissue culture studies.

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Amination of Polystyrene Microwells: Application to the Covalent Grafting of DNA Probes for Hybridization Assays

Cited by 60 publications

References 11 publications

A Novel Electrochemical DNA Biosensor Fabricated with Layer‐by‐Layer Covalent Attachment of Multiwalled Carbon Nanotubes and Gold Nanoparticles

A Novel Electrochemical DNA Biosensor Fabricated with Layer‐by‐Layer Covalent Attachment of Multiwalled Carbon Nanotubes and Gold Nanoparticles

Direct hapten coated immunoassay format for the detection of atrazine and 2,4-dichlorophenoxyacetic acid herbicides

Stability of plasma-polymerized allylamine films with sterilization by autoclaving

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