Direct hapten coated immunoassay format for the detection of atrazine and 2,4-dichlorophenoxyacetic acid herbicides

Kaur, Jasdeep; Boro, Robin Chandra; Wangoo, Nishima; Singh, Kumar Rajesh; Suri, C. Raman

doi:10.1016/j.aca.2007.11.017

Cited by 62 publications

(30 citation statements)

References 28 publications

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“…1,2 To control and contain such a hazardous pollutant, a sensitive method of detection of this organic toxin is urgently required. [6][7][8][9] Various immunoassay formats such as fluoroimmunoassay, surface plasmon resonance immunosensor, fluorescence polarization and strip-test have been reported for the detection of 2,4-D. [10][11][12][13] Recently, gold nanoparticles (GNPs) have attracted keen interest because of their unique size-dependent optical and electronic properties as well as great analytical potential. 3,4 However, these methods of detection of pesticides are complex and time-consuming and even require costly and bulky instrumentation.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticles catalyzed chemiluminescence immunoassay for detection of herbicide 2,4-dichlorophenoxyacetic acid

Boro

Kaushal

Nangia

et al. 2011

Analyst

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We present a novel immunoassay format utilizing the catalytic properties of gold nanoparticles in the luminol-silver nitrate-gold nanoparticle based chemiluminescence (CL) system for the detection of widely used herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Highly sensitive anti-2,4-D antibody was produced and conjugated with gold nanoparticles of various sizes. In the present assay format, employing a competitive inhibition approach, a well-characterized hapten-protein conjugate (2,4-D-BSA) was used to coat the microtiter plates. The analyte (2,4-D) was pre-incubated with anti-2,4-D antibody labeled with gold nanoparticles and added to each well of the microtiter plate. The gold label triggered the reaction between luminol and silver nitrate generating a luminescence signal at 425 nm. Under the optimized conditions, the CL based immunoassay showed the detection limit of 2,4-D in standard water samples around 3 ng mL(-1). The CL based immunoassay format, based on gold nanoparticles as a catalyst, could be used as a fast screening methodology (<30 min) for pesticide detection.

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Section: Introductionmentioning

confidence: 99%

Gold nanoparticles catalyzed chemiluminescence immunoassay for detection of herbicide 2,4-dichlorophenoxyacetic acid

Boro

Kaushal

Nangia

et al. 2011

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“…Moreover, different authors supported that direct hapten coated format showed an improvement in immunoassay sensitivity as well; this probably due to changes in the valency of antibody-hapten interaction (Gerdes et al, 1999;Townsend et al, 2006). In line with this, several approaches have been developed on modified polystyrene (PS) microtriter wells of ELISA plates, resulting in improved analytical performances of the assays (Böcher and Sorensen, 1994;Feng et al, 2009;Kaur et al, 2008;Holthues et al, 2005;Niveleau et al, 1993).…”

Section: Introductionmentioning

confidence: 89%

Direct hapten-linked multiplexed immunoassays on polycarbonate surface

Tamarit-López¹,

Morais²,

Puchades³

et al. 2011

Biosensors and Bioelectronics

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Direct hapten-linked multiplexed immunoassay is developed on the polycarbonate surface of standard Digital Versatile Discs (DVDs) for six compounds of environmental concern, as proof of concept. Carboxylated haptens are directly linked to the aminated polycarbonate surface through carbodiimide/succinimide coupling. The modified DVD surface maintained its physical and optical properties. Multiplexed assay reached detection limits down to 0.1 μg/L for chlorpyrifos, 2,4,5-trichlorophenoxypropionic acid, sulfathiazole and sulfasalazine and down to 1.0 μg/L for fenthion and malathion. This approach presents advantages such as the improvement in sensitivity in comparison to protein-hapten conjugate format for all the studied analytes and the absence of cross-interference effects, allowing high throughput multianalysis on the same surface. Also, a comparison of the performance of two sensing strategies indicated that DVD disc and drive approach turned out in a simpler mode, the assays being more reproducible and with higher signal to noise ratios.

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“…Immunoassays can meet these requirements. Numerous related methods for the detection of atrazine have been reported, such as enzyme-linked immunosorbent assay (ELISA) [10][11][12][13][14][15], colloidal gold-based immunochromatographic (ICG) strip assay [16][17][18], time-resolved fluorescent immunoassay (TRFIA) [19] and liposome immune lysis assay (LILA) [20]. The specific features of each study are listed and compared in Table 1.…”

Section: Introductionmentioning

confidence: 99%

Enzyme-linked immunosorbent assay and immunochromatographic strip for rapid detection of atrazine in water samples

Sheng

Yuan

et al. 2012

Microchim Acta

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We have developed a heterologous direct competitive enzyme-linked immunosorbent assay (ELISA) and a colloidal gold-based immunochromatographic (ICG) strip for the determination of the herbicide atrazine in water samples. The ELISA had a half-maximum inhibition concentration (IC 50 ) of 0.12 ng mL −1 and a limit of detection (LOD, calculated as the IC 15 value) of 0.01 ng mL −1 . The average of recoveries for all spiked water samples was 96.5%. There was a good correlation between the data determined by this ELISA and those obtained by high performance liquid chromatography (HPLC) (r 2 00.996). The visual LOD of the ICG strip assay was 2 ng mL −1 . The assay process only took 10 min, and no sample pretreatment was required. Its high specificity, sensitivity and fast detection made the strip well suited for on-site screening of atrazine in water samples. Both the ELISA and the ICG strip assay are useful for rapid analysis of a large number of water samples at low cost.

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Direct hapten coated immunoassay format for the detection of atrazine and 2,4-dichlorophenoxyacetic acid herbicides

Cited by 62 publications

References 28 publications

Gold nanoparticles catalyzed chemiluminescence immunoassay for detection of herbicide 2,4-dichlorophenoxyacetic acid

Gold nanoparticles catalyzed chemiluminescence immunoassay for detection of herbicide 2,4-dichlorophenoxyacetic acid

Direct hapten-linked multiplexed immunoassays on polycarbonate surface

Enzyme-linked immunosorbent assay and immunochromatographic strip for rapid detection of atrazine in water samples

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