A Novel Electrochemical DNA Biosensor Fabricated with Layer‐by‐Layer Covalent Attachment of Multiwalled Carbon Nanotubes and Gold Nanoparticles

Ma, Haiyan; Zhang, Liping; Pan, Yan; Zhang, Keying; Zhang, Yuzhong

doi:10.1002/elan.200704169

Cited by 61 publications

(29 citation statements)

References 41 publications

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“…The excellent electrical conductivity, large surface area, significant mechanical strength, surface chemical reactivity and pore structure make CNTs attractive materials for electroanalysis. Since CNTs can easily be adsorbed on the surface of glassy carbon electrodes, they have been studied extensively for a large variety of applications, particularly for solid-state chemical and biological sensors [17,18].…”

Section: Introductionmentioning

confidence: 99%

Direct Electrochemical Determination of Glyphosate at Copper Phthalocyanine/Multiwalled Carbon Nanotube Film Electrodes

et al. 2010

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A copper phthalocyanine/multiwalled carbon nanotube film-modified glassy carbon electrode has been used for the determination of the herbicide glyphosate (Gly) at À 50 mV vs. SCE by electrochemical oxidation using differential pulse voltammetry (DPV). Cyclic voltammetry and electrochemical impedance spectroscopy showed that Gly is adsorbed on the metallic centre of the copper phthalocyanine molecule, with formation of Gly-copper ion complexes. An analytical method was developed using DPV in pH 7.4 phosphate buffer solution, without any pretreatment steps: Gly was determined in the concentration range of 0.83 -9.90 mmol L À1, with detection limit 12.2 nmol L À1 (2.02 mg L À1).

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Section: Introductionmentioning

confidence: 99%

Direct Electrochemical Determination of Glyphosate at Copper Phthalocyanine/Multiwalled Carbon Nanotube Film Electrodes

et al. 2010

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“…[19,20] Our groups have reported several electrochemical DNA biosensors based on carbon nanotubes and Au NPs amplifying detection signal. [21,22] Cupric oxide (CuO) nanoparticles are a kind of semiconductor nanomaterials, and it has been used for various sensors such as hydrazine, [23] glucose. [24] As well known, the nanomaterials of different dimensions and shape affected the performance of the biosensor.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of a Sensitive Electrochemical Biosensor for Detection of DNA Hybridization Based on Gold Nanoparticles/CuO Nanospindles Modified Glassy Carbon Electrode

2012

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In this work, a sensitive electrochemical DNA biosensor for the detection of sequence-specific target DNA was reported. Firstly, CuO nanospindles (CuO NS) were immobilized on the surface of a glassy carbon electrode (GCE). Subsequently, gold nanoparticles (Au NPs) were introduced to the surface of CuO NS by the electrochemical deposition mode. Probe DNA with SH (HS-DNA) at the 5'-phosphate end was covalently immobilized on the surface of the Au NPs through Au-S bond. Scanning electron microscopy (SEM) was used to elucidate the morphology of the assembled film, and electrochemical impedance spectroscopy technique (EIS) was used to investigate the DNA sensor assembly process. Hybridization detection of DNA was performed with differential pulse voltammetry (DPV) and the methylene blue (MB) was hybridization indicator. Under the optimal conditions, the decline of reduction peak current of MB (ΔI) was linear with the logarithm of the concentration of complementary DNA from 1.0× 10 -13 to 1.0×10 -6 mol•L -1 with a detection limit of 3.5×10 -14 mol•L -1 (S/N＝3). In addition, this DNA biosensor has good selectivity, and even can distinguish single-mismatched target DNA.

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“…Abouzar and his coworkers have developed a label-free electrical detection of DNA hybridization functionalized with AuNPs [16]. Our groups have fabricated several DNA biosensor based on AuNPs amplification [17][18][19]. Castañeda et al have a review on electrochemical sensing of DNA using AuNPs [20].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Sequence-Specific DNA with the Amplification of Gold Nanoparticles

Zhang

Wang

et al. 2011

International Journal of Electrochemistry

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A sensitive electrochemical DNA biosensor was prepared based on mercaptoacetic acid (MAA)/gold nanoparticles (AuNPs) modified electrode. Probe DNA (NH 2 -DNA) was covalently linked to the carboxyl group of MAA in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxyl-succinimide (NHS). Scanning electron microscopy (SEM) and electrochemical impedance spectra (EIS) were used to investigate the film assembly process. The DNA hybridization events were monitored by differential pulse voltammetry (DPV), and adriamycin was used as the electrochemical indicator. Also the factors influencing the performance of the DNA hybridization were investigated in detail. Under the optimal conditions, the signal was linearly changed with target DNA concentration increased from 5.0 × 10 −13 to 1.0 × 10 −9 M and had a detection limit of 1.7 × 10 −13 M (signal/noise ratio of 3). In addition, the DNA biosensor showed good reproducibility and stability during DNA assay.

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A Novel Electrochemical DNA Biosensor Fabricated with Layer‐by‐Layer Covalent Attachment of Multiwalled Carbon Nanotubes and Gold Nanoparticles

Cited by 61 publications

References 41 publications

Direct Electrochemical Determination of Glyphosate at Copper Phthalocyanine/Multiwalled Carbon Nanotube Film Electrodes

Direct Electrochemical Determination of Glyphosate at Copper Phthalocyanine/Multiwalled Carbon Nanotube Film Electrodes

Fabrication of a Sensitive Electrochemical Biosensor for Detection of DNA Hybridization Based on Gold Nanoparticles/CuO Nanospindles Modified Glassy Carbon Electrode

Electrochemical Detection of Sequence-Specific DNA with the Amplification of Gold Nanoparticles

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