Aktivitas Antimikroba Ekstrak Bakau (Rhizophora mucronata) dalam Menghambat Pertumbuhan Ralstonia Solanacearum Penyebab Penyakit Layu

Egra, Saat; Mardhiana,; Rofin, Mut; Adiwena, Muh; Jannah, Nur; Kuspradini, Harlinda; Mitsunaga, Tohru

doi:10.21107/agrovigor.v12i1.5143

Cited by 18 publications

(23 citation statements)

References 2 publications

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“…Tanaman mangrove (Rhizophora mucronata) memiliki senyawa metabolit sekunder berupa alkaloid, tannin, dan flavonoid (Kasitowati et al, 2017). Menurut (Egra et al, 2019) aktivitas antibakteri ditemukan dalam tumbuhan Rhizophora mucronata mengandung senyawa aktif triterpenoid, flavonoid, alkaloid, dan tanin.…”

Section: Pendahuluanunclassified

Isolation and Identification of Secondary Metabolic Compounds from Mangrove (Rhizophora mucronata) and their Bioactivity Against Escherichia coli and Staphylococcus aureus Bacteria

Ligina

Sudarmin

2022

ijcs

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Bacteria are often found as the cause of disease in the human body because they are pathogenic. One of the bacteria that cause infectious diseases is Escherichia coli and Staphylococcus aureus. Mangrove plants that are rich in secondary metabolites are used to obtain antibacterial substances that can play a role in inhibiting bacterial growth. The purpose of this study was to determine the presence of secondary metabolites of alkaloids in mangrove plants (Rhizophora mucronata) and to determine the antibacterial activity of alkaloid compounds in inhibiting the growth of Staphylococcus aureus and Escherichia coli bacteria. The research method was carried out by remaceration and isolation of alkaloid compounds, analysis of thin-layer chromatography (TLC) and column chromatography, and characterization tests using FT-IR and UV-Vis. The results of the analysis showed the presence of isoquinoline-derived alkaloids supported by FT-IR absorption at 3317 cm-1 indicating the N-H functional group which is a characteristic of alkaloids and the UV-Vis wavelength of 230 nm. These compounds can inhibit the growth of Staphylococcus aureus bacteria by 2 nm and Escherichia coli by 0 nm. The inhibition obtained against Escherichia coli bacteria was caused by the cell membrane layer of gram-negative bacteria consisting of 3 layers so that it was resistant to antibacterial and difficult to penetrate. Staphylococcus aureus only has a single layer, so it is more easily penetrated by antibacterials by causing lysis and inhibiting DNA intercalation.

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Section: Pendahuluanunclassified

Isolation and Identification of Secondary Metabolic Compounds from Mangrove (Rhizophora mucronata) and their Bioactivity Against Escherichia coli and Staphylococcus aureus Bacteria

Ligina

Sudarmin

2022

ijcs

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“…Senyawa bioaktif tanaman seperti metabolit sekunder telah banyak diteliti memiliki sifat antimikroba (Isah, 2019;Yogabaanu et al, 2017). Egra et al (2019) menyatakan bahwa ekstrak daun dan kulit batang tanaman bakau (Rhizophora mucronata) mengandung senyawa flavonoid, tannin, alkaloid dan terpenoid yang berpotensi sebagai antimikroba terhadap Ralstonia solanacearum.…”

Section: Pendahuluanunclassified

“…Akan tetapi, aktivitas tersebut tergantung dari jenis antibiotik yang digunakan. Sebagai antijamur, senyawa metabolit khususnya alkaloid dapat merusak membran sel sehingga menghasilkan perubahan permeabilitas sel, perubahan pengangkutan dan homeostasis serta perubahan susunan dinding sel yang mengarah pada terhambatnya pertumbuhan sel atau kematian sel (Egra et al, 2019 ;Friska et al, 2021). Berdasarkan riset Sant et al (2016) senyawa metabolit seperti fenol juga dapat mendenaturasi protein sel, mengganggu metabolisme mikrobia, serta melisiskan dinding sel.…”

Section: Pendahuluanunclassified

Potential of Orchids Secondary Metabolites as Anti-Cancer and Antimicrobial Based on Prediction of Phytochemical Activity with Online PASS-Software

Yasmin

Mafiroh

Kinasih

et al. 2022

AMS

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Abstract Orchidology is part of the branch of botany, it deals with a large family of monocotyledonous plants, Orchidaceae. Orchids have been used as traditional herbal medicines in Indonesia which are believed to be antimicrobial and anti-cancer. Orchids are able to produce secondary metabolites as their protective agent due to the extreme environment. Orchids have great diversity and potential to be the object of research, not only in experimental studies but also in a computational studies like in silico. Nowadays, molecular or metabolite data are available on the official-standard website as an international database. This study was conducted by analyzing web-based data to provide information about the potential of orchids that have been trusted as herbal medicines. In this study, secondary metabolite from Vanilla spp., Dendrobium spp., and Vanda spp., were selected in the existing literature as antimicrobial and anti-cancer drugs. Secondary metabolites obtained from the KNApSAck-3D core system and Phytochemical and Prediction of Substance Activity Spectrum (PASS) were performed to determine the potential anti-cancer and antimicrobial activity. Based on in silico analysis through PASS online, the secondary metabolites of orchids that have potential as antimicrobials in this study were Dendroside E and Dendromonilised D from Dendrobium also Parviflorin, Licoisoflavone and Luteine extracted from Vanda spp. The secondary metabolite of Vanilla planifolia which has potential as anti-cancer for further research is 4-Hydroxybenzoic acid as a Chlordecone reductase inhibitor extracted from shoots. Keywords: orchid, antimicrobial, anticancer, secondary metabolite, PASS online

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“…Mekanisme penghambatan pertumbuhan bakteri oleh senyawa terpenoid adalah terpenoid bereaksi dengan porin (protein transmembran) pada membran luar dinding sel bakteri membentuk ikatan polimer yang kuat sehingga mengakibatkan rusaknya porin. Kerusakan porin merupakan pintu keluar masuknya substansi, sehingga mengurangi permeabilitas dinding sel bakteri yang akan mengakibatkan sel bakteri akan kekurangan nutrisi maka pertumbuhan bakteri terhambat atau mati [45].…”

Section: Gambar 3 Hubungan Konsentrasi Minyak Atsiri Kulit Buah Jeruk Kalamansi Terhadap Diameter Zona Hambatunclassified

Aktivitas Antibakteri Kulit Buah Jeruk Kalamansi (Citrofortunella Microcarpa) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli

Amiliah

Nurhamidah

Handayani

2021

atp

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This study aims to test the antibacterial activity of crude extracts and essential oils of citrus fruit peel calamansi against bacteria Staphylococcus auerus and Escherichia coli. Calamansi citrus fruit raw material is obtained from the village of Pondok Kubang, Bengkulu Tengah (3.70660 S, 102.35780 E). The crude extract of citrus fruit peel obtained from the maceration result using 96% etanol, furthermore crude extracts were obtained diluted concentrations of 40%, 20%, 10%,and 5%. The essential oil of citrus fruit peel is obtained from the distillation of stem-water, then made variation of concentration 20%, 15%, 10%, and 5%. The method used to test antibacterial activity is the method of paper disc diffusion. The antibacterial activity is shown by the presence on inhibitory zone diameter. The data of antibacterial test result were analyzed by using One Way Anova which showed the effect of treatment on test bacteria growth seen from (P<0,01) and continued by Duncan test to know the effect of treatment. The results showed that the crude extract of citrus fruit peel had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is 10 mm and 7.2 mm (medium) at concentration of 40%. The essential oil of citrus fruit peel has antibacterial activity against S. Aureus and E. coli bacteria with each inhibitory zone diameter 12,7 mm (strong) and 8,3 mm (medium) at concentration 20%.

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Aktivitas Antimikroba Ekstrak Bakau (Rhizophora mucronata) dalam Menghambat Pertumbuhan Ralstonia Solanacearum Penyebab Penyakit Layu

Cited by 18 publications

References 2 publications

Isolation and Identification of Secondary Metabolic Compounds from Mangrove (Rhizophora mucronata) and their Bioactivity Against Escherichia coli and Staphylococcus aureus Bacteria

Isolation and Identification of Secondary Metabolic Compounds from Mangrove (Rhizophora mucronata) and their Bioactivity Against Escherichia coli and Staphylococcus aureus Bacteria

Potential of Orchids Secondary Metabolites as Anti-Cancer and Antimicrobial Based on Prediction of Phytochemical Activity with Online PASS-Software

Aktivitas Antibakteri Kulit Buah Jeruk Kalamansi (Citrofortunella Microcarpa) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli

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