A simple and rapid HPLC/UV method for the simultaneous quantification of theophylline and etofylline in human plasma

Nirogi, Ramakrishna; Kandikere, Vishwottam; Shukla, Manoj K.; Mudigonda, Koteshwara; Ajjala, Devender Reddy

doi:10.1016/j.jchromb.2006.10.035

Cited by 34 publications

(22 citation statements)

References 24 publications

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“…The described methods were validated in terms of linearity, limit of detection (LOD), limit of quantification (LOQ), recovery, selectivity, stability, precision and accuracy according to FDA guidelines regarding standard bioanalytical method validation recommendation [12] Linearity: The analytical range to be validated was chosen on the basis of the expected plasma concentrations of the studied drugs [13][14][15][16][17][18]. The calibration curve was done for each analyte in the biological sample.…”

Section: Methods Validationmentioning

confidence: 99%

HPLC and Densitometric TLC Methods for Simultaneous Determination of Gemifloxacin with Some Co-administered Drugs in Human Plasma

Abdallah¹

2014

J Chromat Separation Techniq

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Two chromatographic methods have been developed for determination of gemifloxacin in human plasma with three co-administered drugs, theophylline, warfarin and omeprazole. First method depends on reverse phase high performance liquid chromatography. The plasma sample was extracted using acetonitrile. The method was linear over the concentration range 0.05 to 6 µg/mL, 0.25 to 8 µg/mL, 0.1 to 10 µg/mL and 0.1 to 6 µg/mL of gemifloxacin mesylate, theophylline, warfarin and omeprazole, respectively. The mobile phase used was prepared by mixing acetonitrile and 0.02 mol L-1 potassium dihydrogen phosphate buffer (pH adjusted to 2.5 using ortho phosphoric acid) in a ratio 10:90 with the addition of 1% TEA and flow rate 1 mL/min in isocratic mode and UV-detection at wavelength 254 nm. Second method depends on densitometric thin layer chromatography. The method was linear over concentration range 0.1 to 3 µg/ml, 0.5 to 6 µg/mL, 0.2 to 2.5 µg/mL and 0.1 to 1.5 µg/mL of gemifloxacin mesylate, theophylline, warfarin and omeprazole, respectively. The mobile phase was selected as mixture of dichloromethane, methanol and ammonia in the ratio of (7: 5.5: 3 v/v/v) for the development of plates. Densitometric analysis was carried out at wavelength 254 nm. The stability of gemifloxacin mesylate and the co-administered drugs in plasma was confirmed during three freeze-thaw cycles (−20°C).

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Section: Methods Validationmentioning

confidence: 99%

HPLC and Densitometric TLC Methods for Simultaneous Determination of Gemifloxacin with Some Co-administered Drugs in Human Plasma

Abdallah¹

2014

J Chromat Separation Techniq

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“…Nagraju and Kaza (2009) reported HPLC for analysis of theophylline and salbutamol in bilayer sustained release. HPLC was also used for rapid analysis for the simultaneous quantification of theophylline and etophylline in human plasma (Nirogi et al, 2007). Ramakrishna et al (2007) used a simple and rapid HPLC/UV method for the simultaneous quantification of theophylline and etophylline in human plasma.…”

Section: Introductionmentioning

confidence: 99%

Validation and Quantification of Theophylline and Salbutamol using Ion Pair Liquid Chromatography

Sophi

Martono

Rohman

2016

Indonesian J. Pharm.

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Salbutamol and theophylline are administered in combination orally to provide the sinergistic effect of drugs. The aim of this study is to develop and to validate ion pair liquid chromatography to determine both drugs in pharmaceutical dosage form, especialy in syrup. Separation of drugs was performed with Spherisorb C-18 column (250x4.6mm; 10um). The mobile phase used was the mixture of acetic acid 1% : methanol (60:40v/v) which contain 3.5mM sodium-1-octanesulphonate, as ion pairing reagent. The mobile phase was delivered isocratically with flow rate of 1mL/min. UV detection was set at the wavelength of 277nm. The developed method was validated in terms of linearity, precision, accuracy, selectivity, and sensitivity. The precision of the method was evaluated using repeatability assay having relative standard deviation (RSD) values of 0.41-0.70% for theophylline and 0.08-0.24% for salbutamol. The recovery percentages were in the range of 98.28-101.02%(theophylline) and 100.71-101.60% for salbutamol. The developed method me t the validation requirement for analysis of salbutamol and theophylline simultaneously in syrup dosage form. Furthermore, the method also provided acceptable result for syrup and tablet dosage forms containing Salbutamol and theophylline only, and tablet containing the combination of Salbutamol and theophylline.

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“…A number of analytical separation methods have been employed to determine theophylline in plasma such as: gas chromatography with mass spectrometry (GC/MS) [22], high-performance liquid chromatography with ultraviolet detection (HPLC-UV) [23][24][25][26][27], MS (LC/MS) [28][29][30], and electrochemical detection (HPLC-ECD) [31][32][33][34], capillary electrophoresis [35], and immunoassay [36][37][38][39][40]. Although HPLC represents a common and powerful technique for the analysis of biological samples, all the methods, except for HPLC-UV, are not always useful for pharmacokinetics.…”

Section: Introductionmentioning

confidence: 99%

Prepeak of trolox caused by theophylline and its application to the determination of theophylline in rat plasma

Kotani

Hashimoto

Kotani

et al. 2011

Journal of Electroanalytical Chemistry

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A simple and rapid HPLC/UV method for the simultaneous quantification of theophylline and etofylline in human plasma

Cited by 34 publications

References 24 publications

HPLC and Densitometric TLC Methods for Simultaneous Determination of Gemifloxacin with Some Co-administered Drugs in Human Plasma

HPLC and Densitometric TLC Methods for Simultaneous Determination of Gemifloxacin with Some Co-administered Drugs in Human Plasma

Validation and Quantification of Theophylline and Salbutamol using Ion Pair Liquid Chromatography

Prepeak of trolox caused by theophylline and its application to the determination of theophylline in rat plasma

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