Validation and Quantification of Theophylline and Salbutamol using Ion Pair Liquid Chromatography

Sophi, Shanti Laksmi; Martono, Sudibyo; Rohman, Abdul

doi:10.14499/indonesianjpharm27iss4pp190

Cited by 5 publications

(3 citation statements)

References 3 publications

(3 reference statements)

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“…Many methods have been reported for the determination of SBS, these include HPLC, Reverse phase high performance liquid chromatography (4,6,7,8) , achiral supercritical fluid chromatography (9), ion pair liquid chromatography (10),atomic absorption spectrophotometric (11), Differential pulse voltammetric (12), titrimetric method (13), continuous/stopped flow injection method (14), spectrophotometric methods (1, 15,16), first-order derivative spectroscopy (17) and Second Order Derivative method (18). The purpose of the present study is to develop a simple and sensitive method for the determination of salbutamol sulphate in pharmaceutical preparations using diazotization coupling reaction.…”

Section: Figure 1 the Chemical Structure Of Salbutamolmentioning

confidence: 99%

Spectrophotometric Assay of Salbutamol Sulphate in Pharmaceutical Preparations by Coupling with Diazotized ρ-bromoaniline

Tamathir¹

2019

Baghdad Sci.J

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In this research, salbutamol sulphate (SAS) has been determined by a simple, rapid and sensitive spectrophotometric method. Salbutamol sulphate in this method is based on the coupling of SAS with diazotized ρ- bromoaniline reagent in alkaline medium of Triton X-100 (Tx) to form an orange azo dye which is stable and water-soluble. The azo dye is exhibiting maximum absorption at 441 nm. A 10 - 800 µg of SAS is obeyed of Beer's law in a final volume of 20 ml, i.e., 0.5- 40 ppm with ε, the molar absorptivity of 48558 L.mol-1.cm-1 and Sandell's sensitivity index of 0.01188 µg.cm-2. This new method does not need solvent extraction or temperature control which is well applied to determine SAS in different types of pharmaceutical preparations.

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Section: Figure 1 the Chemical Structure Of Salbutamolmentioning

confidence: 99%

Spectrophotometric Assay of Salbutamol Sulphate in Pharmaceutical Preparations by Coupling with Diazotized ρ-bromoaniline

Tamathir¹

2019

Baghdad Sci.J

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“…Various analytical techniques have been reported for quantifying theophylline by UVspectrophotometric and RP-HPLC methods. Reported crucial approaches are the determination of theophylline in pharmaceutical dosage forms by buffer-free high-performance liquid chromatography method [12], validation and quantification of theophylline and salbutamol using ion-pair liquid chromatography [13], HPLC and UV method for the estimating of theophylline in rabbit plasma [14], RP-HPLC method for simultaneous estimation of salbutamol sulphate and theophylline in pharmaceutical syrup dosage form [15], development and validation of UV spectrophotometric methods for simultaneous estimation of amlodipine besylate and theophylline [16].…”

Section: Introductionmentioning

confidence: 99%

A Promising Analytical Method has been Crafted, Validated, and Quantified for Estimating Theophylline Utilizing UV Spectroscopic and RP-HPLC Techniques in Conjunction with Stress Degradation Studies to Pinpoint Deterioration

Madhavi

Mondal

Chakraborty

et al. 2023

J. Pharm. Res. Int.

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Introduction: The current study aimed to develop and assess UV-spectrophotometric (zero order, first order, second order, area under the curve) and RP-HPLC methods for estimating theophylline in its pharmaceutical dosage form. Methods: A less toxic solvent composition of acetonitrile and 0.1% orthophosphoric acid (25:75 v/v) is used as a mobile phase and diluent for developing both UV-spectroscopic and RP-HPLC techniques. Shimadzu Prominence LC-20A Modular HPLC system with a C18 column (250 × 4.6 mm; 5 µm) was used to develop the RP-HPLC method. Results: Method A is a zero-order spectrophotometric method for determining theophylline at 271 nm, and the correlation coefficient in the linearity study was found to be 0.9958, LOD, and LOQ are 0.52 and 1.71 µg/mL. Method B is a first-order spectrophotometric method for determining theophylline at 258 nm, and the correlation coefficient in the linearity study was found to be 0.9983, LOD, and LOQ are 0.46 and 1.51 µg/mL. Method C is a second-order spectrophotometric method for determining theophylline at 218 nm, and the correlation coefficient in the linearity study was found to be 0.9941, LOD, and LOQ are 0.38 and 1.25 µg/mL. Method D is an area under the curve spectrophotometric method for determining theophylline at 260 to 282 nm, and the correlation coefficient in the linearity study was found to be 0.9978, LOD, and LOQ are 0.57 and 1.88 µg/mL. Method E is the RP-HPLC method for the determination of theophylline at the retention time of 2.814 min, and the correlation coefficient in the linearity study was found to be 0.9923, LOD, and LOQ are 0.78 and 2.57 µg/mL. Studies on stress degradation show that oxidation and acid degradation mostly impact theophylline solutions. Conclusion: Theophylline can be determined using the proposed approach, which is convenient, precise, affordable, and reproducible.

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“…Theophylline and salbutamol were validated using ion-pair liquid chromatography by S. L. Sophi. A mixture of acetic acid and methanol (60:40v/v) which contains 3.5mM sodium-1-octane sulphonate (anionic IPR) was used as mobile phase [ 18 ]. Ion-pair isocratic simultaneous determination of Fluoroquinolones in environmental samples was described by L. Hlabangan and S. Memeza using HPLC with UV detection.…”

Section: Introductionmentioning

confidence: 99%

Ionisable substances chromatography: A new approach for the determination of Ketoprofen, Etoricoxib, and Diclofenac sodium in pharmaceuticals using ion – pair HPLC

Andraws

Trefi

2020

Heliyon

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An ion-pair HPLC method was developed and validated to analyze three of non-steroidal anti-inflammatory drugs (Ketoprofen, Etoricoxib, and Diclofenac sodium) in their pure and pharmaceuticals based on their ionisable characteristics. Cetyltrimethylammonium bromide (Cetrimide) was used as an ion pair reagent since it had not been used before for this purpose. Chromatographic analysis was accomplished using the C18 (250 × 4.6 mm, 5μm) column. Mobile phase consisted of a mixture of 50% Cetrimide M and 50% acetonitrile to analyze Ketoprofen and Etoricoxib, whereas for Diclofenac sodium, mobile phase was a mixture of 30% Cetrimide M and 70% acetonitrile. pH value was adjusted if necessary to 10 with ammonium hydroxide. The flow rate was 1mL/min and detection wavelengths were at 254 nm, 234 nm, and 254 nm for Ketoprofen, Etoricoxib, and Diclofenac sodium; respectively under ambient temperature. Retention times ( ) were 9.41, 7.34, and 6.66 for Ketoprofen, Etoricoxib, and Diclofenac sodium; respectively. The proposed method was evaluated for linearity, accuracy, precision, and specificity according to ICH guidelines. Ketoprofen, Etoricoxib, and Diclofenac sodium were detected in the following linear ranges: (0.031–0.500mg/mL), (0.007–0.110g/mL), and (0.016–0.250mg/mL); respectively with excellent mean recovery values (98.0–102.0%). RSD% was in an acceptable range (less than 2), proving the precision of the developed method. Specificity was proved in the presence of degradation products. Furthermore, a comparison between the results of this study and the reported HPLC methods indicated that this developed method was better in terms of simplicity, analysis time, and no use of buffers in the mobile phase. In conclusion, the developed method can successfully detect Ketoprofen, Etoricoxib, and Diclofenac sodium quantitatively and qualitatively in their dosage forms without any interference with excipients, making this method valuable, reliable, and practical to be applied in quality control laboratories.

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Validation and Quantification of Theophylline and Salbutamol using Ion Pair Liquid Chromatography

Cited by 5 publications

References 3 publications

Spectrophotometric Assay of Salbutamol Sulphate in Pharmaceutical Preparations by Coupling with Diazotized ρ-bromoaniline

Spectrophotometric Assay of Salbutamol Sulphate in Pharmaceutical Preparations by Coupling with Diazotized ρ-bromoaniline

A Promising Analytical Method has been Crafted, Validated, and Quantified for Estimating Theophylline Utilizing UV Spectroscopic and RP-HPLC Techniques in Conjunction with Stress Degradation Studies to Pinpoint Deterioration

Ionisable substances chromatography: A new approach for the determination of Ketoprofen, Etoricoxib, and Diclofenac sodium in pharmaceuticals using ion – pair HPLC

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