HPLC and Densitometric TLC Methods for Simultaneous Determination of Gemifloxacin with Some Co-administered Drugs in Human Plasma

Abstract

Two chromatographic methods have been developed for determination of gemifloxacin in human plasma with three co-administered drugs, theophylline, warfarin and omeprazole. First method depends on reverse phase high performance liquid chromatography. The plasma sample was extracted using acetonitrile. The method was linear over the concentration range 0.05 to 6 µg/mL, 0.25 to 8 µg/mL, 0.1 to 10 µg/mL and 0.1 to 6 µg/mL of gemifloxacin mesylate, theophylline, warfarin and omeprazole, respectively. The mobile phase used was prepared by mixing acetonitrile and 0.02 mol L-1 potassium dihydrogen phosphate buffer (pH adjusted to 2.5 using ortho phosphoric acid) in a ratio 10:90 with the addition of 1% TEA and flow rate 1 mL/min in isocratic mode and UV-detection at wavelength 254 nm. Second method depends on densitometric thin layer chromatography. The method was linear over concentration range 0.1 to 3 µg/ml, 0.5 to 6 µg/mL, 0.2 to 2.5 µg/mL and 0.1 to 1.5 µg/mL of gemifloxacin mesylate, theophylline, warfarin and omeprazole, respectively. The mobile phase was selected as mixture of dichloromethane, methanol and ammonia in the ratio of (7: 5.5: 3 v/v/v) for the development of plates. Densitometric analysis was carried out at wavelength 254 nm. The stability of gemifloxacin mesylate and the co-administered drugs in plasma was confirmed during three freeze-thaw cycles (−20°C).

Development of Rp-HPLC Method for Simultaneous Determination of Nebivolol Hydrochloride, Valsartan and Hydrochlorothiazide in Co-Administrated Antihypertensive Agents and Human Plasma

“…The drugs were extracted from plasma by applying sample preparation technique which leads to decrease the effect of plasma protein and other plasma matrix components that could interfere with determination of the studied drugs.1.50 mL of acetonitrile was added to 0.50 mL plasma for precipitation of the plasma protein 15 . %Recoveries were found to be 97.27%, 99.74% and 98.21% for HCZ, NEB and VAL dosage forms respectively.…”

Fabrication and Applications of Potentiometric Membrane Sensors Based on Specific Recognition Sites for the Measurement of the Quinolone Antibacterial Drug Gemifloxacin

“…According to a literature review, analytical methods used for the determination of gemifloxacin include spectrophotometry [ 6 , 7 , 8 ], spectrofluorometry [ 9 , 10 ], polarography [ 11 ], voltammetry [ 12 , 13 , 14 ], HP-TLC [ 15 , 16 ], HPLC-UV [ 17 , 18 , 19 ] , HPLC-F [ 20 , 21 , 22 ], HPLC-MS [ 23 , 24 , 25 ], and potentiometry [ 26 , 27 , 28 , 29 ].…”

Supramolecular interaction of gemifloxacin and hydroxyl propyl β-cyclodextrin spectroscopic characterization, molecular modeling and analytical application

“…1). Few analytical methods appeared in the literature for the determination of this drug including spectrophotometric [21][22][23][24], LC [25][26][27], HPLC [28,29], electrophoresis [30,31] and spectrofluorimetry [32,33] were reported for analysis of GFX.…”