A sensitive assay for clavulanic acid and sulbactam in biological fluids by high-performance liquid chromatography and precolumn derivatization

RP-HPLC Method Development and Validation for Estimation of Acebrophylline

“…The identifying peak should have a reasonable retention time and should be well separated from extraneous peaks at the detection levels which the assay will be performed. 17,18…”

A liquid chromatographic method for simultaneous determination of amoxicillin sodium and sulbactam sodium in a combination formulation

“…The methods published for the determination of sulbactam sodium are LC [19][20][21], enzymatic method [22] and spectrophotometry [24]. Most of the LC methods with UV detection were used for the individual determination of the two drugs in biological samples and complex chromatographic conditions were involved such as chiral stationary phase [12], on-line postcolumn [13] or precolumn [21] derivatisation, column switching [14] or coupled column [15], paired-ion aqueous mobile phase [16][17][18] and gradient elution [17,18]. Two and sulbactam sodium in pharmaceutical preparations [25,26].…”

Quick bioanalytical method of quantification and validation of sulbactam in plasma using high performance liquid chromatography

“…For practical applications, derivatization step has been performed. This additional step has lead to lowering the detection limit and the limit of quantification based on concentration [29]. Derivatives of drugs are usually detected at different wavelengths which can increase sensitivity and allow the detection at lower concentrations.…”

“…Many methods of analysis of sulbactam in biological fluids and pharmaceutical formulations using high performance liquid chromatography have been developed [15][16][17][18]. Moreover, the United States Pharmacopoeia (USP) recommends the use of liquid chromatographic methods in determination of sulbactam in raw materials and in its dosage forms [14].…”