Quick bioanalytical method of quantification and validation of sulbactam in plasma using high performance liquid chromatography

Abstract

Abstract:A quick method of quantitative determination of sulbactam in human plasma, using liquid chromatography-UV spectroscopy, has been developed and validated. After derivatization with imidazole, plasma samples were treated by direct deproteinization with acetonitrile as an extraction solvent. After ultracentrifugation, sulbactam extract was directly injected onto the LC column.Chromatographic separation was performed on TSK Gel Super ODS (50 mm × 4.6 mm i.d., 2 µm) using methanol and phosphate buffer with tetrabutylammonium hydroxide solution as a mobile phase. Gradient elution was employed. The method was fully validated according to the United States Food and Drug Administration requirements (linearity, precision, trueness, quantification limit, detection limit, recovery, specificity and stability). The calibration curves were linear within the concentration range of 0.05-4.0 μg mL -1. Good method/system precision and accuracy of the method were demonstrated. Warsaw and Springer-Verlag Berlin Heidelberg. © Versita

Population Pharmacokinetics and Pharmacodynamics Modeling To Optimize Dosage Regimens of Sulbactam in Critically Ill Patients with Severe Sepsis Caused by Acinetobacter baumannii

“…Blood concentrations of sulbactam were determined by reverse-phase HPLC. The samples were prepared by the modified method described by Abu-Shandi (13). Briefly, 200 l of 2 M imidazole was added to 500 l of plasma sample, which was vortexed for 10 s, and then the mixture was kept at 60°C for 50 min to allow complete derivatization and finally cooled at room temperature for 15 min.…”

Improving antimicrobial use in critically ill patients

“…In recent years, published methods for analysis of multiple antibiotics in plasma by ultra high performance liquid chromatography-mass spectrometry (UHPLC)/mass spectrometry (UHPLC-MS/MS) and high performance liquid chromatography-ultra-violet (HPLC-UV) have included ampicillin, although without analysis of sulbactam [213][214][215][216][217][218]. Sulbactam has been measured in plasma alone and with antibiotics other than ampicillin [219][220][221][222].…”