A novel metabarcoded 18S ribosomal DNA sequencing tool for the detection of Plasmodium species in malaria positive patients

Haemoprotozoan surveillance in peri-urban native and introduced wildlife from Australia

“…(2019) (17.0%). However, more widespread comparisons are difficult to make as in many cases sequences retained throughout bioinformatics are not adequately reported ( Squarre et al., 2020 ; Wahab et al., 2020 ). Due to the nature of the Illumina sequencing platform, artificial sequences or chimeras can be generated, particularly when libraries are prepared by PCR amplification methods.…”

“…In addition, the amplification biases of primers and the ability to differentiate species varies between the hypervariable regions. As a result, studies on pathogen or parasite prevalence still largely use these semi-targeted approaches ( Ghafar et al., 2020 ; Wahab et al., 2020 ). A semi-targeted molecular approach using primers targeting conserved regions of the nuclear 18S rRNA locus provides a number of advantages over using generic eukaryote primers ( Bradley et al., 2016 ; Cannon et al., 2018 ).…”

Diagnostic Methods for Non-Falciparum Malaria

“…The assay proved to be sensitive and specific for Pf (n=36) and Pv (n=17) detection, while the other species were not assayed. The strategy of sequencing amplicons for determination of the species present in malariapositive human blood samples was also proposed using the Illumina MiSeq platform to quantify Pf and Pv by PCR (Wahab et al, 2020). However, this platform (mainly the required equipment) is relatively expensive and lacks desired characteristics for PoC settings.…”

Current methods for the detection of Plasmodium parasite species infecting humans

“…Because these smears are not usually fixed, blood cells undergo haemolysis, leaving only parasites and leukocytes visible. Thin smears are often used for species identification and estimating parasitemia, as it is easier to observe individual Plasmodium parasites in this preparation ( Wahab et al., 2020 ). The trained technicians or health professionals can also detect the morphological differences in Plasmodium spp., dependent on the life-cycle stage of the parasite.…”