A new bovine adenovirus

Abstract

In the United States Klein, Early and Zellat (1959) and Klein, ZeUat and Michaelson (1960) isolated the bovine adenovirus type 1 and type 2.In England Darbyshire, Dawson, Lamont, Ostler, and Pereira (1965) isolated bovine adenovirus type 3 and in Hungary Bartha and Alddsy (1966) isolated bovine adenovirus type 4 and type 5.In our laboratory an adenovirus has been isolated as a latent virfi~s : from primary calf testes cell cultures. Its cytopathic effect is slow and the virus titre is low e.g. 102.5 TCIDs0/0.1 ml in the third passage. The virus produces basophilic intranuclear inclusion bodies ( Fig.) which resemble the nuclear changes produced by adenoviruses. The virus does not multiply and does not produce a cy~opathic effect in primary calf kidney cell cultures, primary pig kidney cell cultures, PK15 cell line, a calf kidney cell line, a pig kidney cell line and HeLa cell line. The virus-synthesis is inhibited by 5-iodo-2-deoxyuridine. The virus resists treatment with ether, chloroform, trypsine and sodium desoxycholate and is also resistant to heat for one hour at 50~ but not resistant to heat for half an hour at 56~The heat inactivation is not stabilized by two molar sodium chloride or magnesium chloride. There is no loss in infectivity titre when the virus suspension is held at pH 3 or pH 9 for 3 hours at room temperature (22~The virus does not agglutinate red blood cells of horse, pig, calf, sheep, goat, guinea pig, human 0, chicken, mouse and rat at 4~ and 37~ It passes through a membrane filter (Sartorius ~r filter, G6ttingen) with an average pore diameter of 150 mfz , but not through one of 100 m~. _Applying the factor of 0.64 (Black, 1958) to the

Isolation and characterization of bovine adenovirus type 5 associated with ?weak calf syndrome?

“…Isolations were from apparently healthy cattle (9) and from a variety of clinical syndromes that include conjunctivitis (24), pneumonia (7), pneumoenteritis (1), and diarrhea (14). Latent adenoviruses have also been isolated from primary bovine testes (4,23) and bovine embryonic kidney cell cultures (20).…”

A bovine adenovirus isolated from lymph nodes of cattle

“…TANAKA and coworkers (11) and I•ABA et al (5) propagated bovine adenovirus type 4 and another isolate to be differentiated (8) from blood of calves with rhinitis, diarrhea and fever. RONDtIUIS (9) and SoHoPov et al (10) found contaminating bovine adenoviruses in cell cultures from calf kidney and testis. The latter strains were not related to serotypes 1--5, and were independently registered as serotype 6.…”

Adenoviruses of Vertebrate Animals

“…Many bovine adenoviruses have been isolated from apparently normal animals. The B6 prototype strain came from a calf testes cell culture (Rondhuis, 1968), as did B4 strains (Bartha and Csontos, 1969) and B4 and B6 (Phillip and Sands, 1972) strains. Isolations have also been made under conditions not associated with adenoviruses, such as the isolation of a type 6 virus from the lymph node of a leukotic cow (Mayr et al, 1970).…”