SummaryThe preparation and maintenance of a culture of bovine turbinate cells is described. The cell line is free of bovine viral diarrhea, parainfluenza-3, and infectious bovine rhinotracheitis viruses ; and although the modal chromosome number changed significantly between the 9th passage and the 81st passage, no definite morphological changes were observed. Low-and high-passage bovine turbinate cells were equally susceptible to bovine viral diarrhea virus.
Bovine viral diarrhea virus (BVDV) was concentrated and purified by a combination of ultrafiltration, hydroextraction using polyethylene glycol and affinity chromatography. A lectin from Crotalaria juncea that has an affinity for galactose was used in the affinity chromatography. Virions of BVDV with classic envelopes were observed by electron microscopy. Four major proteins with estimated molecular weights of 75,000, 66,000, 54,000, and 26,000 were identified in sodium dodecyl sulfate--polyacrylamide gel electrophoresis slab gels. The proteins of 75,000 and 54,000 were glycoproteins as shown by staining with dansyl hydrazine.
SummaryAn adenovirus isolated from a calf with clinical signs of polyarthritis or "weak calf sivldrome" was characterized and identified as bovine adenovirus type 5. This is the first time this serotype has been described in the United States.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.