A device for the rapid transfer/transplantation of living cell sheets with the absence of cell damage

Tadakuma, Kenjiro; Tanaka, Nobuyuki; Haraguchi, Yuji; Higashimori, Mitsuru; Kaneko, Makoto; Yamato, Masayuki; Okano, Teruo

doi:10.1016/j.biomaterials.2013.08.006

Cited by 35 publications

(18 citation statements)

References 39 publications

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“…multiblock copolymer structure) and the thickness of grafted PNIPAAm immobilized covalently onto TCPS (Elloumi‐Hannachi et al ., ), inducing temperature alterations of hydrophilic/hydrophobic properties and cell adhesion/detachment (Akiyama et al ., ; Yamato et al ., ; Yamato et al ., ). So far, many articles have also reported disadvantages of NIPAAm‐grafted dishes regarding biocompatibility, low mechanical properties and lack of flexibility (Hinds et al ., ; Tadakuma et al ., ). Investigation of functional thermoresponsive hydrogels as supports for cell sheet production have gained interest for their ease of handling, inexpensive synthetic process, ease of functionalization and drug‐release ability, representing a promising alternative to NIPAAm‐grafted dishes (Martins et al ., ; Fujita et al ., ).…”

Section: Introductionmentioning

confidence: 99%

P(NIPAAM-co-HEMA) thermoresponsive hydrogels: an alternative approach for muscle cell sheet engineering

Villa

Martello

Erratico

et al. 2014

J Tissue Eng Regen Med

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Loss of skeletal muscle tissue caused by traumatic injury or damage due to myopathies produces a deficit of muscle function for which there is still no clinical treatment. Transplantation of myogenic cells, themselves or combined with materials, has been proposed to increase the regenerative capacity of skeletal muscle but it is hampered by many limitations, such as low cell survival and engraftment or immunological reaction and low biocompatibility of the exogenous materials. Recently, myoblast sheet engineering, obtained with thermoresponsive culture dishes, has attracted attention as a new technique for muscle damage treatment. For this purpose, a series of thermoresponsive hydrogels, constituted by poly(N-isopropylacrylamide-co-2-hydroxyethylmethacrylate) [p(NIPAAM-co-HEMA)] were synthesized by a simple and inexpensive free-radical polymerization of the two co-monomers with a redox initiator. Different ratios of N-isopropylacrylamide (NIPAAm) and 2-hydroxyethylmethacrylate (HEMA) have been examined to evaluate the effects on physicochemical, mechanical and optical hydrogel properties. The murine muscle cell line C C has been exploited to test the cytotoxicity of the thermoresponsive hydrogels, depending on different synthesis conditions. In this study, we have identified a thermoresponsive hydrogel that allows cell adhesion and viability, together with the detachment of viable sheet of muscle cells, giving the chance to develop further applications for muscle damage and disease. Copyright © 2014 John Wiley & Sons, Ltd.

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Section: Introductionmentioning

confidence: 99%

P(NIPAAM-co-HEMA) thermoresponsive hydrogels: an alternative approach for muscle cell sheet engineering

Villa

Martello

Erratico

et al. 2014

J Tissue Eng Regen Med

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“…10 Most recently, a cell sheet transfer device has been developed, which has a scooping part to transfer cell sheets by a movable belt and a handling part to operate the device. 11 However, all of these methods require applying a direct force on the cell sheet, which may affect cell viability, activities, long-term functions and cause variation between users.…”

Section: Introductionmentioning

confidence: 99%

Stacked stem cell sheets enhance cell-matrix interactions

Patel

Zhang

2014

Organogenesis

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“…The cell culturing of mouse skeletal myoblast cell line C2C12 was performed as described elsewhere [16]. Briefly, Dulbecco's modified Eagle's Medium (DMEM) (A6429; Sigma-Aldrich, St. Louis, MO) supplemented with 10v/v% fetal bovine serum (FBS) (Nichirei Biosciences, Tokyo, Japan) and 1v/v% penicillin-streptomycin solution (168-23191; Wako Pure Chemical, Osaka, Japan) was prepared as a culture medium.…”

Section: Cell Culturingmentioning

confidence: 99%

Contamination-free non-contact wettability assessment system

Tanaka¹,

Haruzono

Nasu

et al. 2017

Robomech J

Self Cite

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Surface wettability is one of most important indexes for understanding the interfacial characteristics between a solid surface and a liquid, especially such physico-chemical properties as hydrophilicity and water repellency. Recently, non-contact wettability assessment by monitoring the behavior of liquid covering a sample surface by jet-flow application to the liquid has been proposed. This method is applicable to the in-liquid assessment of high wettability materials for which it is difficult to measure the contact angle, such as cultured cells in a liquid culture medium. However, when the flow is dirty, the contamination of the cell culture system by particles (e.g. microorganisms, virus, and aerosols) may occur. To solve this problem, we developed a contamination-free non-contact wettability assessment system that is composed of a clean-air source having a particle filter and an automated system for image processing instrumentation and motorized alignment device for sample positioning. We confirmed that the filtered air-jet contained no particles, although over ten thousand particles had been included in the air source. Furthermore, we detected no microorganisms on agar culture plates, which had been exposed to the air-jet before being incubated. With the image instrumentation, we automatically measured the liquid-squeezed size, which was the main index of surface wettability. The motorized alignment device allowed us to control the sample position without human direct operation. This developed system is compatible with wettability assessment in a high-level clean environment such as quality control of culture cells for regenerative medicine and in vitro assay of drug efficacy and safety.

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A device for the rapid transfer/transplantation of living cell sheets with the absence of cell damage

Cited by 35 publications

References 39 publications

P(NIPAAM-co-HEMA) thermoresponsive hydrogels: an alternative approach for muscle cell sheet engineering

P(NIPAAM-co-HEMA) thermoresponsive hydrogels: an alternative approach for muscle cell sheet engineering

Stacked stem cell sheets enhance cell-matrix interactions

Contamination-free non-contact wettability assessment system

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