Stacked stem cell sheets enhance cell-matrix interactions

Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

“…The use of cell sheets can improve the survival rate of therapeutic cells and allows them to easily attach to host tissues. Moreover, the cell-matrix and cell-cell interactions in an intact sheet play an essential role in maintaining their cell genomic stability as 'building blocks' and their biochemical signaling and modulation of stem cell behavior toward therapeutic regeneration (Patel and Zhang 2014;Wu et al 2015). Most importantly, cell sheets make delivering and securing PDLSCs onto the tooth root surface possible, which is a key to PDL tissue regeneration (Dan et al 2014).…”

Age-related decline in the matrix contents and functional properties of human periodontal ligament stem cell sheets

“…Cell sheet engineering has been established as an important cell delivery strategy that enables the fabrication of viable, transplantable cell sheets to ensure robust transplantation of therapeutic cells, along with their deposited ECM contents, without the aid of scaffolding materials [17][18][19]. Following transplantation, cells within an intact sheet are easily attached to host tissues, with minimal cell loss.…”

“…Importantly, the maintenance of cell-cell and cell-ECM interactions ensures appropriate cell responsiveness to surrounding cues and physiological signals for wound repair and regeneration. From the grafting of a temperature-responsive polymer onto the surface of a cell culture dish to create a cell sheet [52,53] and the coating of temperature-responsive culture dishes with ECM proteins such as laminin-5 [54] to the use of various adjuvants, such as ascorbic acid phosphate [24] and osthole [23], that confer cell sheet production without the need for special materials, this technology exhibits great potential to enhance tissue regeneration and wound healing [18,55]. In periodontal regenerative medicine, PDLSC sheets have been shown to substantially restore tissues damaged by periodontitis in swine and to form a bone/cementum-like matrix [8,19,24,35].…”

“…In recent years, cell sheet engineering has rapidly progressed to allow the development of confluent cell sheets for delivering therapeutic cells along with their in vitro-secreted extracellular matrix (ECM) in the field of regenerative medicine [17,18]. In using this unique, scaffold-free method of cell processing, the preservation of cell-growing microenvironments in terms of various mechanical, chemical, and biological properties as well as cell-matrix and cell-cell interactions may minimize cell loss and increase cell survival rate and engraftment after cell sheet transplantation [17].…”

Organ-on-a-Chip Platforms for Drug Delivery and Cell Characterization: A Review

“…(19)(20)(21)(22) The most common 3D cell culturing platforms are cell aggregates, (21) spheroids, (19) and cell sheets. (18,22) Examples of cell aggregates and multicell-type sheets are shown in Figs. 1(b) and 1(c), respectively.…”