A Design of Experiment Approach to the Synthesis of Alendronate-incorporated Hydroxyapatite

The potential of lignosulfonates as widely underutilized byproducts of the pulp and paper industry for the synthesis of a biodegradable pesticide carrier system was assessed in this study. Design of experiment software MODDE Pro was for the first time applied to optimize lignosulfonate granule production using Myceliophthora thermophila laccase as a biocatalyst. Enzymatic cross-linking was monitored using size exclusion chromatography coupled online to multiangle laser light scattering, viscosity measurement, and enzyme activity. The determined optimal and experimentally confirmed incubation conditions were: 33 °C, 30 cm3/min O2 supply, and 190 min reaction time. The granules were thereafter loaded with 2 g/kg 3,6-dichloro-2-methoxybenzoic acid (Dicamba), a broad-spectrum herbicide. According to the HPLC analysis, complete release of Dicamba was achieved after 48 h of release. This study showed the green production of a 100% lignosulfonate-based biodegradable solid carrier with potential application in agriculture.

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“…Molecular weight and viscosity were chosen as responses. This method is readily applied in many fields for similar applications. , …”

Section: Methodsmentioning

confidence: 99%

Lignin-Based Pesticide Delivery System

Weiß

Ghitti

Sumetzberger-Hasinger

et al. 2020

ACS Omega

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“…A composite with a high incorporation efficiency of the drug was subsequently obtained. Furthermore, the hydroxyapatite appeared as a single crystalline phase [24].…”

Section: Synthesis and Characterization Of The Synthesized Hydroxyapamentioning

confidence: 99%

Evaluation of Mesenchymal Stem Cells and Osteoblasts’ Adhesion and Proliferation in the Presence of HA-AL Biomaterials

et al. 2019

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There is an increased interest in developing biocomposite implants with high biocompatibility in order to be used as grafts or prostheses in orthopedic surgery. The purpose of the study was to determine the biocompatibility of titanium implants coated with synthesized hydroxyapatite-alendronate composites. The implants were obtained using Matrix Assisted Pulsed Laser Evaporation technique (MAPLE). The hydroxyapatite-alendronate composites were synthesized using the wet precipitation method. Immunofluorescence microscopy showed that composites support mesenchymal stem cells (MSCs) adhesion. Bone cells as well as human MSCs adhere to hydroxyapatite (HA)-based thin films obtained by matrix assisted laser deposition onto titanium. Alendronate doping into the films increased the number of cell-biomaterial focal points as compared to HA only. Thus, the synthesis of hydroxyapatite-alendronate composite (HA-AL) may be considered a viable solution for including the bisphosphonate on the surface of metallic prosthetic components used in orthopedics.

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“…Four variables, namely gentamycin concentration, PLGA concentration, buffer concentration and stirring rate were evaluated in relation to nanoparticle size and encapsulation efficiency. A Full Factory (2 levels) interaction model experimental design was implemented using quality by design (QbD) approach, which is widely promoted by the Food and Drug Administration and the International Conference on Harmonization (ICH) [22,23]. Furthermore, a degradation study was conducted to evaluate the PLGA 65:35 used for the synthesis of gentamicin loaded nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Process Optimization Using Quality by Design (Qbd) Approach of a Gentamicin Loaded Plga Biocomposite

et al. 2021

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Osteomyelitis continues to be a major concern when orthopedic surgery is performed. Orthopedic infections have an incidence of 5% to 10% but their management proves to be quite difficult due to both biofilm formation and limited access of the drug to the infected area when systemic treatment is employed. The aim of the study was to optimize the synthesis process of a gentamicin loaded poly(-lactic-co-glycolic) acid (PLGA) based biodegradable composite by varying parameters that affect both efficiency encapsulation and nanoparticle size. Furthermore, a kinetic study was conducted to study the biodegradation process of the polymer. Gentamicin loaded PLGA nanoparticles were obtained using the double emulsion technique which allows the variation of several factors such as gentamycin concentration, PLGA concentration, buffer concentration and stirring speed. Out of the four factors evaluated, gentamicin concentration had the highest impact on both encapsulation efficiency and nanoparticle size. A few relevant interactions between factors were also registered.

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A Design of Experiment Approach to the Synthesis of Alendronate-incorporated Hydroxyapatite

Cited by 7 publications

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Lignin-Based Pesticide Delivery System

Lignin-Based Pesticide Delivery System

Evaluation of Mesenchymal Stem Cells and Osteoblasts’ Adhesion and Proliferation in the Presence of HA-AL Biomaterials

Process Optimization Using Quality by Design (Qbd) Approach of a Gentamicin Loaded Plga Biocomposite

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