Acquiring multiple signals along with the reaction time: improving recognition capability of a multidimensional colorimetric sensor array for sensitive protein detection

Abstract

The development of sensitive and cheap sensor arrays for identification of proteins plays an important role in many bioanalytical and clinical investigations. Here, we introduce a multidimensional colorimetric sensor array for the detection of multiple proteins based on acquiring multiple signals along with the reaction time to enhance the discrimination ability. In a single experiment, the unique fingerprint for each protein against the sensor array is generated from a response absorbance signal at three reaction time points (at 10 min, 15 min, and 20 min). Our colorimetric sensing system is able to identify ten proteins not only in aqueous solution at 10 nM but also in human urine at the 50 nM level with an accuracy of 100%. Moreover, the identification of HSA in urine at the nanomolar level within a linear range of 0.05-1.0 μM is achieved. Our sensing array system is sufficiently sensitive for the discrimination of pure HSA, binary mixtures of HSA and Lys at a total concentration of 50 nM in urine. This study indicates that the application of the real-time resolved response signals enables the enhancement of the discrimination ability for protein recognition.

Array-Based Discriminative Optical Biosensors for Identifying Multiple Proteins in Aqueous Solution and Biofluids

“…Thus, such a sensor was able to effectively identify 13 proteins, BSA and HSA mixtures, unknown samples and proteins in urine with an accuracy of 100%. They also developed another triple-channel colorimetric sensor by collecting the absorbance signals at three different reaction time points (10, 15, and 20 min) (Yang et al, 2017 ). Ten proteins in both aqueous solution and human urine were discriminated with 100% accuracy.…”

Colorimetric sensor arrays for antioxidant recognition based on Co3O4 dual-enzyme activities

“…For example, Chen et al reported colorimetric sensor arrays based on three nanozymes, namely, graphene oxide, molybdenum disulfide (MoS 2 ) and tungsten disulfide (WS 2 ) as sensing elements for antioxidant detection. 18 As reported, most colorimetric array sensors were constructed using nanomaterials, 19 pH, 20 metal ions, 21,22 fluorescence, 20,23,24 and time 25 as sensing elements. However, there is no report on the colorimetric array based on one nanozyme with the double enzyme-like activities.…”

Fluorescence Sensor Array for Discrimination of Urine Proteins and Differentiation Diagnosis of Urinary System Diseases

“…However, discrimination of complex mixture is still a great challenge for sensor array-based sensing systems due to the complex responses and intervention of various analytes. For example, although some sensor arrays have achieved pretty high sensitivity and accuracy in detection of single protein samples; − few reports have been published on the differentiation between complex protein samples and proteins in biological samples.…”