In order to establish a rapid, accurate, and simple method
for
monitoring and analyzing the biological physiological state, we constructed
a colorimetric sensor array based on the peroxidase-like activity
of nanozymes (CoMoO4 and Por-CoMoO4) for the
determination of five kinds of biothiols. The peroxidase-like activity
of CoMoO4 has been greatly enhanced by the introduction
of porphyrin (Por), due to the synergistic interaction between porphyrin
and CoMoO4. Experimental data of the radical scavenger
and fluorescence probe verify the generation of a superoxide radical
(•O2
–) during the catalytic reaction.
Because different biothiols have different inhibiting effects on the
catalytic activity of two nanozymes, each biothiol has a unique fingerprint
with the aid of a chromogenic substrate (TMB). Through linear discriminant
analysis, captopril (CAP), cysteine (Cys), homocysteine (Hcy), dithiothreitol
(DTT), and glutathione (GSH) can be well identified in a certain range
of concentrations. Different concentrations of biothiols and different
proportions of mixed biothiols can be identified. It is expected that
this study will provide a simple and rapid method for the identification
of structural analogues, contributing to the monitoring of human life
and health.
A sensor array based on the double enzyme–like activity of Co3O4 nanozymes was constructed to identify four different antioxidants. Due to the double enzyme–like activity of Co3O4, oxTMB appears blue with different degrees in the presence and absence of H2O2.
A simple colorimetric sensing platform based on the excellent peroxidase-like activity of Zn3V2O7(OH)2·2H2O nanorods at physiological pH to detect H2O2 and epinephrine.
Constructing of sensing platform based on nanoenzymes has been focused by scientists from the different countries. Due to Phosphate ion (Pi) is considered as an important indicator for water eutrophication,...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.