Detection and quantification of Leishmania braziliensis in ectoparasites from dogs

Morais, Rayana Carla Silva de; Gonçalves-de-Albuquerque, Suênia da Cunha; Silva, Rômulo Pessoa e; Costa, Pietra Lemos; Silva, Kamila Gaudêncio da; Silva, Fernando José da; Brandão-Filho, Sinval Pinto; Dantas‐Torres, Filipe; Paiva-Cavalcanti, Milena de

doi:10.1016/j.vetpar.2013.03.026

Cited by 5 publications

(2 citation statements)

References 14 publications

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“…In our opinion, the flea gut is the most likely place for such an exchange. Indeed, trypanosomatids can survive in a nonspecific insect host for a considerable period ( 32 , 53 ), and even Leishmania parasites were detected in adult fleas ( 54 ). Both Leishmania and Blechomonas may have enough time for contacts, and they are separated by no barriers in both imagos and larvae.…”

Section: Resultsmentioning

confidence: 99%

RNA Viruses in Blechomonas (Trypanosomatidae) and Evolution of Leishmaniavirus

Grybchuk

Kostygov

Macedo

et al. 2018

mBio

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Flagellates belonging to the genus Leishmania are important human parasites. Some strains of different Leishmania species harbor viruses (leishmaniaviruses), which facilitate metastatic spread of the parasites, thus aggravating the disease. Up until now, these viruses were known to be hosted only by Leishmania. Here, we analyzed viral distribution in Blechomonas, a related group of flagellates parasitizing fleas, and revealed that they also bear leishmaniaviruses. Our findings shed light on the entangled evolution of these viruses. In addition, we documented that Blechomonas can be also infected by leishbunyaviruses and narnaviruses, viral groups known from other insects’ flagellates.

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Section: Resultsmentioning

confidence: 99%

RNA Viruses in Blechomonas (Trypanosomatidae) and Evolution of Leishmaniavirus

Grybchuk

Kostygov

Macedo

et al. 2018

mBio

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“…En Perú, R. microplus se ha reportado como un parásito del ganado y, en Brasil, R. sanguineus se considera un parásito de perros, así como potencial vector de L. infantum (19)(20)(21) y L. braziliensis (20). Debe señalarse, además, que en estudios anteriores en Italia y Brasil se encontraron porcentajes más Figura 3.…”

Section: Discussionunclassified

Detection of Leishmania (V) guyanensis in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) collected from Pecari tajacu

et al. 2017

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Contribución de los autores:Jesús E. Rojas-Jaimes, Germán H. Correa-Nuñez: concepción y diseño del estudio, recolección e interpretación de los datos y redacción del manuscrito Nyshon Rojas-Palomino: diseño del estudio molecular para identificación de especies de Leishmania y análisis de resultados Omar Cáceres-Rey: diseño del estudio molecular Todos los autores participaron en la revisión crítica del manuscrito. Detección de Conclusion:The results showed the presence of L. (V) guyanensis DNA in R. microplus possibly acquired after biting a collarde peccary. Therefore, it is important to design future studies to clarify R. microplus involvement in the transmission of leishmaniasis.

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Cutaneous leishmaniosis in naturally infected dogs in Paraná, Brazil, and the epidemiological implications of Leishmania (Viannia) braziliensis detection in internal organs and intact skin

Márquez

Castro

Nabut

et al. 2017

Veterinary Parasitology

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Environmental changes have occurred over the years, altering the eco-epidemiological pattern of leishmaniosis in the State of Paraná, Brazil, involving the pillars of the cycle (parasite, vectors, reservoir, and environment) and their interaction. Much has been discussed about the dog's role as a reservoir of the Leishmania (Viannia) braziliensis Vianna, 1911 transmission cycle. However, this question remains unanswered. The purpose of this study was to investigate, using parasitological and molecular methods, different samples in eight naturally infected dogs from an endemic rural locality where only L. (V.) braziliensis is present, and where human cases have been previously notified. Blood and biopsied organ samples from naturally infected dogs were analyzed by culture media, PCR, random amplified polymorphic DNA and sequencing methodologies. Only skin lesions from all dogs yielded positive cultures and when PCR was performed, L. (V.) braziliensis DNA was amplified from intact skin, peripheral blood, bone marrow, spleen, liver and lymph nodes. RAPD was also applied to isolates from the skin lesions, exhibiting the genetic variability of the parasite identified. To confirm which species of Leishmania was amplified in PCR, the sequencing method was performed, verifying 100% similarity with the Viannia subgenus. This study showed that L. (V.) braziliensis can spread to other sites besides the ulcerous lesions, such as intact skin, peripheral blood and internal organs, making it possibility for dogs to serve as active sources of parasite transmission. For definitive proof, xenodiagnostic test on intact skin of infected dogs, should be done.

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Detection and quantification of Leishmania braziliensis in ectoparasites from dogs

Cited by 5 publications

References 14 publications

RNA Viruses in Blechomonas (Trypanosomatidae) and Evolution of Leishmaniavirus

RNA Viruses in Blechomonas (Trypanosomatidae) and Evolution of Leishmaniavirus

Detection of Leishmania (V) guyanensis in Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) collected from Pecari tajacu

Cutaneous leishmaniosis in naturally infected dogs in Paraná, Brazil, and the epidemiological implications of Leishmania (Viannia) braziliensis detection in internal organs and intact skin

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