Application of Cytochrome Oxidase I Sequences for Phylogenetic Analysis and Identification of Thrips Species Occurring on Vegetable Crops

Abstract

Thrips are direct pests as well as vectors of important viruses infecting crop plants. One of the major constraints in studying the relationship between thrips vectors and tospoviruses is the difficulty of identifying the vector species because of high intraspecific variation among thrips populations. Molecular approaches have been used to identify species differences. In this study, partial cytochrome oxidase I (COI) sequences were used to understand the phylogenetic relationship among thrips populations, and assess their usefulness to identify and classify unknown thrips species collected from different crops. In total, 29 COI variants were obtained while examining the sequence polymorphisms in COI of 182 insects analyzed in this study, which were collected from six countries on tomato, chilli, onion, cabbage, cucumber, watermelon, Ethiopian mustard, French bean, and peanut. The phylogenetic analysis showed that the insects used in this study clustered with five distinct species-groups designated as Thrips palmi group, T. tabaci group, Frankliniella occidentalis group, Scirtothrips dorsalis group and an unclassified group. Higher intraspecific genetic variation was observed in S. dorsalis and T. palmi followed by T. tabaci and F. occidentalis. Thus, it was confirmed that the COI gene could be useful in grouping different thrips species and genera that coexist in a particular cropping system.

Dna Barcoding and Development of Species-Specific Markers for the Identification of Tea Mosquito Bugs (Miridae: Heteroptera) in India

“…viz. T. hawaiiensis and S. perseae along with previously reported cryptic species viz., T. palmi Glover et al 2010), T. tabaci (Brunner et al 2004), F. occidentalis (Rugman-Jones et al 2010), S. dorsalis Kadirvel et al 2013) based on the 'CO-I-10X barcoding Rule' proposed by Hebert et al 2004. All of them were supported by the calculated intra and inter specific genetic divergence (Table.…”

“…In this regard, DNA barcoding can be an added advantage and an effective tool for molecular species identification (Brunner et al 2004;Glover et al 2010; Lee et al 2010), elucidation of biotypes or cryptic species, and host associated genetic differences (Shufran et al 2000;Brunner et al 2004) and species discovery in insects. Additionally, DNA barcoding could also be used in the identification of unknown thrips species that co-exist in a cropping system, since one particular Tospovirus disease may sometimes be vectored by more than one species of thrips (Wijkamp et al 1995;Kadirvel et al 2013;Amin et al 1981). …”

“…Morphological examination of Thrips to species level is restricted to adult specimens, as there are no adequate keys for identification of egg, larvae, or pupae (Kadirvel et al 2013). Thrips are notorious for eliciting taxonomic problems mainly because of minute size, polymorphism (Murai et al 2001), lack of solid morphological characters (Kadirvel et al 2013), co-existence of different species on the same host plant, high intraspecific variations observed in thrips populations (Mound 2011) and need for taxonomic expertise (Brunner et al 2004).…”

Seasonal Changes in Thrips tabaci Population Structure in Two Cultivated Hosts

“…Since Brunner et al’s [7] phylogenic analysis that identified three major genetic lineages for T. tabaci (i.e., Leek 1, Leek 2 and Tobacco strains), numerous similar analyses from around the world have followed [4], [6], [16], [17], [28]. All T. tabaci in our study were from a single leek clade that included individuals collected from an array of hosts representing seven plant families and multiple locations spanning five continents (clade number 2 in Figure 3).…”

Identifikasi Thrips alliorum (Priesner), Thrips hawaiiensis (Morgan), dan Thrips parvispinus (Karny) berdasarkan variasi DNA COI mitokondria

“…Beberapa spesies trips juga dilaporkan sebagai vektor virus. T. palmi dapat menularkan penyakit Groundnut bud necrosis, sementara T. tabaci Lindeman menularkan penyakit Tospovirus (Kardivel et al 2013;Fauziah & Saharan 1991).…”