Dna Barcoding and Development of Species-Specific Markers for the Identification of Tea Mosquito Bugs (Miridae: Heteroptera) in India

Rebijith, K. B.; Asokan, R.; Kumar, N. K. Krishna; Srikumar, K. K.; Ramamurthy, V. V.; Bhat, P. S.

doi:10.1603/en12096

Cited by 28 publications

(10 citation statements)

References 52 publications

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“…Insect identification relies on traditional taxonomy, which is primarily based on external morphology (Rebijith et al 2012). However, taxonomic keys are often prepared for only certain life stages or genders; phenotypic variations in taxonomically important traits may also cause significant difficulties in species identification (Ball et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and identification of three stored grain pests based on mitochondrial cytochrome C oxidase subunit I (COI) gene sequences

Aslam

Sultana

Rain

et al. 2019

Bangladesh J. Zool.

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Stored grain pests are discovered in food as immature stages, which further complicates the identification process. A DNA barcode dataset of some important pests that can be used for easy and confirm identification in stages of life is constructed. COI genes of three stored grain insect pests i.e,, Sitophilus oryzae, Callosobruchus chinensis and Oryzaephilus surinamensis were sequenced. The sequenced genes were submitted to NCBI GenBank and obtained accession numbers MG967331.1, MG967332.1, MG967333.1 and MK041216.1. BLAST analysis showed 99 to 100% homology with existing GenBank sequences. The nucleotide composition analysis revealed that the value of A+T (64.8%) is greater than G+C (35.2%). Genetic distance among four sequences of three store pests were ranged from 0.00293-0.32807. Phylogenetic analysis showed that these three species are originated from different clades. Haplotype analysis of mitochondrial COI gene of the stored grain insect pests showed high genetic diversity among them. C. chinensis, O. surinamensis and S. oryzae were separated from their common ancestor by 80, 73 and 64 mutational steps. These information may be helpful for attempting any successful control measures against the pest species. In conclusion, present author established the first DNA barcode dataset of three store grain pests and confirmed its efficiency for identifying these pests. Bangladesh J. Zool. 47(1): 1-11, 2019

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Section: Introductionmentioning

confidence: 99%

Molecular characterization and identification of three stored grain pests based on mitochondrial cytochrome C oxidase subunit I (COI) gene sequences

Aslam

Sultana

Rain

et al. 2019

Bangladesh J. Zool.

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“…Our search showed that efforts have been initiated to barcode true bugs from India ( Rebijith et al, 2012 ) but were restricted to a few species from a single family Miridae. Therefore the present work was undertaken to identify more species using COI barcodes covering wider taxa range.…”

Section: Introductionmentioning

confidence: 99%

DNA barcoding of Pentatomomorpha bugs (Hemiptera: Heteroptera) from Western Ghats of India

2014

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Recent studies from East Asia and Canadian National Collection of Insects have established the utility of DNA barcoding technique in identification of true bugs. The present study is an expansion of the database by adding mitochondrial cytochrome c oxidase I (mtCOI) sequences from forty three species of indigenous true bugs of India. mtCOI gene analysis of infraorder Pentatomomorpha covering a total of seventy three species that belong to five superfamilies; Pentatomoidea, Coreoidea, Pyrrhocoroidea, Lygaeoidea and Aradoidea revealed more than 3% interspecific distances in all the taxa studied except for two cases which showed barcode sharing. Less than 2% intra-specific divergence was observed in 97% of the taxa analysed and the average interspecies genetic distance was about 29 times higher than the average intraspecies genetic divergence. Distinct sequence divergence pattern at generic level and NJ clustering analysis suggests that COI barcode is an excellent molecular marker for species level identification of unknown taxa; however it may not be useful for resolving deep levels of divergence. Species identification even at nymphal stage could be achieved confirming the efficacy of this technique.

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“…In a phylogenetic analysis based on the mt genome sequences among Lepidoptera, the view that Hyphantria cunea was closely related to Lymantria dispar than Ochrogaster lunifer was supported, as was the hypothesis that Noctuoidea and Geometroidea are monophyletic (Liao et al, 2010). In addition, partial DNA sequence of the mitochondrial gene cytochrome oxidase I (COI) as a barcode, has also been widely used in identifying and discriminating species areas (Jung et al, 2011;Rebijith et al, 2012). These may suggest that the mitochondrial gene is certainly a force to be reckoned with in phylogenetic analysis and molecular marker.…”

Section: Introductionmentioning

confidence: 99%

The mitochondrial genome of the plant bug Apolygus lucorum (Hemiptera: Miridae): Presently known as the smallest in Heteroptera

Wang

et al. 2013

Insect Science

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The complete mitochondrial (mt) genome of the plant bug, Apolygus lucorum, an important cotton pest, has been sequenced and annotated in this study. The entire circular genome is 14 768 bp in size and represents the smallest in presently known heteropteran mt genomes. The mt genome is encoding for two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, 13 protein coding genes and a control region, and the order, content, codon usage and base organization show similarity to a great extent to the hypothetical ancestral model. All protein coding genes use standard initiation codons ATN. Conventional stop codons TAA and TAG have been assigned to the most protein coding genes; however, COIII, ND4 and ND5 genes show incomplete terminator signal (T). All tRNA genes possess the typical clover leaf structure, but the dihydrouridine arm of tRNA(Ser(AGN)) only forms a simple loop. Secondary structure models of rRNA genes are generally in accordance with the former models, although some differences exist in certain parts. Three intergenic spacers have never been found in sequenced mt genomes of Heteroptera. The phylogenetic study based on protein coding genes is largely congruent with previous phylogenetic work. Both Bayesian inference and maximum likelihood analyses highly support the sister relationship of A. lucorum and Lygus lineolaris, and Miridae presents a sister position to Anthocoridae.

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Dna Barcoding and Development of Species-Specific Markers for the Identification of Tea Mosquito Bugs (Miridae: Heteroptera) in India

Cited by 28 publications

References 52 publications

Molecular characterization and identification of three stored grain pests based on mitochondrial cytochrome C oxidase subunit I (COI) gene sequences

Molecular characterization and identification of three stored grain pests based on mitochondrial cytochrome C oxidase subunit I (COI) gene sequences

DNA barcoding of Pentatomomorpha bugs (Hemiptera: Heteroptera) from Western Ghats of India

The mitochondrial genome of the plant bug Apolygus lucorum (Hemiptera: Miridae): Presently known as the smallest in Heteroptera

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