Determination of Pesticides in Grape Juices by QuEChERS and Liquid Chromatography-Tandem Mass Spectrometry

Abstract

In this study, we describe a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying 25 pesticides in grape juice. This method was optimized and validated according with INMETRO (Instituto Nacional de Metrologia Qualidade e Tecnologia) for the simultaneous determination of pesticide, which are widely used in grape culture in Brazil. The method was based on the QuEChERS (quick, easy, cheap, effective, rugged and safe) methodology. We used AOAC Official Method 2007.01 and Standard Method EN 15662 for sample preparation, both methods presented statistically equivalent recoveries and coefficients of variation. At 0.005 and 2 mg L -1 , fortified blank grape juices had average recoveries of 101 and 112% and average coefficients of variation of 16 and 11%, respectively. The repeatability was tested in three concentrations 0.005, 0.1 and 2 mg L -1 , affording coefficients of variation 16.4, 20.7 and 10.8%, respectively.

LC-MS/MS Determination of Pesticide Residues in Fruits and Vegetables

“…Annastassiades et al chose citrate as the buffer and this version was named by the European Committee for Standardization (CEN) as Standard Method EN 15662 [59]. Other frequent modifications include: changes in the composition and/or proportion of the salts used in extraction [60], changes in the composition and/or proportion of the sorbents used [10,12,61], using various proportions of buffers: citrate [12,32,62,63] or acetate [12,32,63] and (in order to maintain the optimum pH of the sample within the 5-5.5 range) using appropriate acids [29,60] or bases [29]. One should also note other variants of the method that are based on omitting the cleanup step completely [40], using the sorbent deposit in the form of a column [64], replacing acetonitrile with ethyl acetate as the organic solvent [65], or introducing an additional step where the analytes are concentrated in the cleaned up extract by evaporating and/or replacing the solvent [61,66,67].…”

Avaliação da ESL-PBT na determinação de carbaril em amostras de abacaxi pérola (ananás comosus) comercializadas no município de Itumbiara

“…O método analítico utilizado pela ANVISA para extração de carbaril em abacaxi, é o QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) [7][8][9]. Este já foi aplicado em diversas amostras de alimentos e bebidas, como: Cebola [10], soja [11], banana, mamão e pimentão [12], maçã, morango e tomate [13], laranja, [14], pera, pepino e repolho [15], mel [16], leite bovino [17], sucos de uva [18] e caldo de cana [19].…”

Simultaneous Voltammetric Detection of Carbaryl and Paraquat Pesticides on Graphene-Modified Boron-Doped Diamond Electrode

“…In order to avoid the health hazards caused by pesticide residues, governments and international organizations regulate the maximum residues level (MRL) in fruit and vegetables, aiming to limit population exposure and to confirm the proper use and exact concentrations of pesticides in agricultural products [ 2 ]. The MRL values established by regulatory bodies range between 0.01–10 mg/kg, in relation to matrices and acceptable daily intake [ 3 , 4 ]. Also, 10 μg L −1 for paraquat and 90 μg L −1 for carbaryl have been reported as guideline limits for water [ 5 ].…”

“…Consequently, trace level detection and fast identification represent important aspects in analytical method selection. In general, pesticides are detected in food and water using chromatographic analytical methods such as gas and liquid chromatography in tandem with mass spectrometry [ 1 , 3 , 4 , 6 ], immunoassays and immunosensors [ 7 ], flow injection chemiluminescence [ 8 ], or fluorescence [ 9 ]. The main disadvantages of these methods are the time consumption, including prerequisite sample pre-treatment/pre-concentration, the expensive instrumentation and the toxic organic reagents required.…”