2014
DOI: 10.5935/0100-4042.20140128
|View full text |Cite
|
Sign up to set email alerts
|

A NEW APPROACH TO EVALUATE IMMOBILIZATION OF β-GALACTOSIDASE ON EUPERGIT®C: STRUCTURAL, KINETIC, AND THERMAL CHARACTERIZATION

Abstract: This study aimed to evaluate β-galactosidase immobilization. For this purpose, the ionic strength of the buffer, reaction time, amount of the immobilization support, and pH were evaluated by a central composite design. Assay 8, which consisted of 1.5 mol L −1 phosphate buffer (pH 7.5) and a reaction time of 2 h, produced the maximum yield. Eupergit ® C (400 mg) was subsequently used as an immobilization support. Immobilization kinetics wereinvestigated, and a significant increase in the yield was obtained afte… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
4
0

Year Published

2019
2019
2024
2024

Publication Types

Select...
4
1
1

Relationship

0
6

Authors

Journals

citations
Cited by 6 publications
(4 citation statements)
references
References 22 publications
0
4
0
Order By: Relevance
“…Original scientific paper Received: May 29, 2018 Accepted: December 5, 2018 stable and possesses good chemical and mechanical properties, such as simple immobilization procedure and high binding capacity 17 . Eupergit C is a naturally porous polymer 18 and immobilization on it can be achieved without the need for additional reagents by forming strong covalent linkages between Eupergit epoxy groups and enzyme nucleophilic groups 19 .…”
Section: Introductionmentioning
confidence: 99%
“…Original scientific paper Received: May 29, 2018 Accepted: December 5, 2018 stable and possesses good chemical and mechanical properties, such as simple immobilization procedure and high binding capacity 17 . Eupergit C is a naturally porous polymer 18 and immobilization on it can be achieved without the need for additional reagents by forming strong covalent linkages between Eupergit epoxy groups and enzyme nucleophilic groups 19 .…”
Section: Introductionmentioning
confidence: 99%
“…To further reduce the cost of applying processes using enzymes, agro-industrial waste available in large quantities can be used in the production of enzymes (Lemes et al, 2016a) using simpler purification protocols or coupling techniques to purify the target product (Lemes et al, 2014;Lemes et al, 2019). Another factor that supports the application of enzymes and cost reduction in the process is the possibility of their immobilization, resulting in their recyclable use, allowing their application in defined cycles, and maintaining their selectivity, catalytic activity, and the generation of products in large quantities (Braga et al, 2014;Basso and Serban, 2019).…”
Section: Extraction Of Bioactive Components With Enzymes From Agro-industrial Wastesmentioning
confidence: 99%
“…[130][131][132] Synthetic polymers often require surface modification to achieve post immobilization. 133 Some commonly used synthetic polymers are PVA, 134 polyvinyl acetate (PVAc), 81 polyimide (PI), 91 polyacrylates (e.g., poly(methyl methacrylate) (PMMA), Eupergit ® -C), 135,136 polyamide (e.g. nylon 6), 137 polyacrylonitrile (PAN), 138 polyaniline (PANi), 139 poly(ethylene glycol) (PEG), 140 and poly(lactic-co-glycolic acid) (PLGA).…”
Section: Msde Reviewmentioning
confidence: 99%