Immobilization of Glucose Oxidase on Eupergit C: Impact of Aeration, Kinetic and Operational Stability Studies of Free and Immobilized Enzyme

Pečar, Darja

doi:10.15255/cabeq.2018.1391

Cited by 8 publications

(4 citation statements)

References 45 publications

(66 reference statements)

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“…The K M and V max values for glucose were 18.15 mM and 2.25 mM min −1 , respectively, which were calculated using the double reciprocal curve. Table S1 lists the K M value in this work and other previously reported results [29][30][31][32][33]. It proves that the embedded glucose oxidase retained relatively good affinity to the substrate of glucose.…”

Section: Evaluation Of Magnetic Embedded Enzymessupporting

confidence: 75%

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine

et al. 2022

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Silica nanoparticles hold tremendous potential for the encapsulation of enzymes. However, aqueous alcohol solutions and catalysts are prerequisites for the production of silica nanoparticles, which are too harsh for maintaining the enzyme activity. Herein, a procedure without any organic solvents and catalysts (acidic or alkaline) is developed for the synthesis of silica-encapsulated glucose-oxidase-coated magnetic nanoparticles by a facile self-assembly route, avoiding damage of the enzyme structure in the reaction system. The encapsulated enzyme was characterized by scanning electron microscopy, transmission electron microscopy, energy-dispersive spectrometry, and a vibrating sample magnetometer. Finally, a colorimetric sensing method was developed for the detection of glucose in urine samples based on the encapsulated glucose oxidase and a hydrogen peroxide test strip. The method exhibited a good linear performance in the concentration range of 20~160 μg mL−1 and good recoveries ranging from 94.3 to 118.0%. This work proves that the self-assembly method could be employed to encapsulate glucose oxidase into silica-coated magnetic particles. The developed colorimetric sensing method shows high sensitivity, which will provide a promising tool for the detection of glucose and the monitoring of diabetes.

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Section: Evaluation Of Magnetic Embedded Enzymessupporting

confidence: 75%

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine

et al. 2022

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“…133 Some commonly used synthetic polymers are PVA, 134 polyvinyl acetate (PVAc), 81 polyimide (PI), 91 polyacrylates ( e.g. , poly(methyl methacrylate) (PMMA), Eupergit ® -C), 135,136 polyamide ( e.g. nylon 6), 137 polyacrylonitrile (PAN), 138 polyaniline (PANi), 139 poly(ethylene glycol) (PEG), 140 and poly(lactic- co -glycolic acid) (PLGA).…”

Section: Enzyme Immobilization Approachesmentioning

confidence: 99%

Enzyme immobilization: polymer–solvent–enzyme compatibility

Asaduzzaman

Salmon

2022

Mol. Syst. Des. Eng.

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“…[130][131][132] Synthetic polymers often require surface modification to achieve post immobilization. 133 Some commonly used synthetic polymers are PVA, 134 polyvinyl acetate (PVAc), 81 polyimide (PI), 91 polyacrylates (e.g., poly(methyl methacrylate) (PMMA), Eupergit ® -C), 135,136 polyamide (e.g. nylon 6), 137 polyacrylonitrile (PAN), 138 polyaniline (PANi), 139 poly(ethylene glycol) (PEG), 140 and Poly(lactic-co-glycolic acid) (PLGA) 141 .…”

Section: Polymer-enzyme Combinations For Post-immobilizationmentioning

confidence: 99%

Enzyme functionalized solution blown nonwovens

Fnu¹,

Salmon²

2022

Preprint

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ASADUZZAMAN, FNU. Enzyme Functionalized Solution Blown Nonwovens. (Under the direction of Dr. Sonja Salmon). This Ph. D. dissertation presents the innovation and development of a new category of functional materials that uses a solution blown spinning (SBS) process to produce novel, useful biocatalytic functionalized nonwovens. SBS is a rapid, mild nanofiber formation process that is not limited to thermal-plastic polymers or high dielectric constant solvent-soluble polymers. With the SBS technique, it was possible to achieve single-step enzyme immobilization into polymeric carriers from enzyme-compatible polymer solutions. The goals of this research were to explore techniques for preparing polymer-solvent-enzyme compatible triads, optimize solution spinning processes for producing enzyme functionalized solution blown nonwovens (EFSBN), and validate the anticipated application features of EFSBN.Chapter 1 is a review of the literature to assess the potential for compatible polymer-solventenzyme triads for co-immobilization solution processing to increase immobilization yield and reduce immobilization complexity. The particular focus is to produce enzyme compatible polymer solutions in an organic solvent for solution blown spinning process where in-situ fiber formation and enzyme immobilization happen. In-situ co-immobilization is one of the promising methods in enzyme immobilization due to its high enzyme loading, versatility, and single-step fabrication processability. However, the stability and compatibility of enzymes in polymer solutions (especially organic solvent-soluble polymer solutions) are challenging. Enzymes may denature in the presence of organic solvents or at elevated temperatures, limiting how enzymes can be combined with organic solvent-soluble polymers. This chapter discusses strategies for producing compatible polymer-solvent-enzyme triads that span aqueous and non-aqueous fabrication requirements.Chapter 2 reports the novel production of unique enzyme functionalized solution blown nonwoven (EFBSN) webs from aqueous soluble polyethylene oxide (PEO) polymer solution by the solution blow spinning method. Protease co-immobilization via entrapment in PEO nanofibers by solution blowing was demonstrated as a simple and efficient process for loading a broad concentration range of enzymes. The rapidly water-soluble and non-dusting EFSBN solid materials preserve a high enzyme activity level over long periods of ambient storage without adding a stabilizer and are easy to handle, making EFSBNs a potential alternative format for delivering enzymes in products that require fast-dissolving solid formulations, like detergents.Chapter 3 reveals an approach to produce an enzyme (CALB) compatible polycaprolactone (PCL)chloroform solution for enzyme co-immobilization via entrapment in PCL nanofibers. CALB enzymes survived in the microemulsion (water-in-oil) of the PCL-chloroform-CALB compatible triad and retained around half of their initial activity after solution blow spinning. These novel CALB-loaded-EFSBN-PCL ...

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Immobilization of Glucose Oxidase on Eupergit C: Impact of Aeration, Kinetic and Operational Stability Studies of Free and Immobilized Enzyme

Cited by 8 publications

References 45 publications

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine

Enzyme immobilization: polymer–solvent–enzyme compatibility

Enzyme functionalized solution blown nonwovens

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