Multiplication of embryogenic calli in Coffea arabica L.

Rezende, Juliana Costa de; Carvalho, C. H. S.; Santos, Ana Carolina Ramia; Pasqual, Moacir; Teixeira, J. B.

doi:10.4025/actasciagron.v34i1.11230

Cited by 7 publications

(9 citation statements)

References 10 publications

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“…The level of concentration of the respective PGRs was observed to have an important impact on the rate of callus induction, with different genotypes displaying different reaction patterns with low concentration levels of 2,4-D (0.11, 0.22 and 0.33 µM) resulting in higher induction rates in code 71 whereas a linear increase in induction rates was observed in Code 93 with increased concentration levels. This is in line with the observations by Molina et al (2002) and Rezende et al (2012) in C. arabica. Overall, Code 93 recorded highest induction rates compared to Code 71 which indicate that induction rates are strongly influenced by genotype which, supporting the observations by Jiménez (2001) that embryogenic response to PGR is directly related to genotype.…”

Section: Callus Inductionsupporting

confidence: 94%

Effects of genotype and plant growth regulators on callus induction in leaf cultures of Coffea arabica L. F1 hybrid

Mwaniki¹,

Lubabali²,

Asava³

et al. 2019

Afr. J. Biotechnol.

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Coffea arabica, F1 hybrid variety, Ruiru 11 is a highly sought after crop in Kenya due to its alleviated resistance to Coffee Berry Disease and Coffee Leaf Rust coupled with high yield capacity and good cup quality. Access to the variety's planting materials is limited due to challenges with difficulty in propagation using conventional methods of seed and vegetative propagation; and somatic embryogenesis is regarded as a suitable alternative propagation method. Therefore, the current study aimed to establish an induction protocol in F1 composite hybrid Ruiru 11. The current study investigated the effects of genotype and plant growth regulators, auxins and cytokinins, on induction of embryogenic callus in two composite genotypes of C. arabica L. F1 hybrid variety Ruiru 11. Leaf explants from the F1 hybrid were cultured on half-strength Murashige and Skoog (MS) media supplemented with varied concentrations and combinations of plant growth regulators. Callus formation was evaluated weekly until the 60 th day. Genotypic effects were assessed based on the difference on callus induction rates, biomass fresh weights and callus formation. The genotypes tested showed highest callus induction 88% (Code 71) and 100% (Code 93) with respect to the formation of embryogenic calli. Highest fresh weight was obtained at 0.973 ± 0.011 g in Code 71 and 0.649 ± 0.03 g in Code 93 in MS media supplemented with 2,4-D + BAP (0.53 + 0.11 µM). The observed results are useful in formulating the best growth regulator concentration suitable for mass in vitro propagation genotypes of Arabica coffee hybrid Ruiru 11 through callus induction in vitro of leaf explants.

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Section: Callus Inductionsupporting

confidence: 94%

Effects of genotype and plant growth regulators on callus induction in leaf cultures of Coffea arabica L. F1 hybrid

Mwaniki¹,

Lubabali²,

Asava³

et al. 2019

Afr. J. Biotechnol.

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“…After counting and measuring, apical and sub-apical orthotropic sprouts were collected, divided in nodal segments of length equal or above 1 cm, and the leaves in each node were cut by their halves, to prepare the micro-cuttings. Microcuttings were planted in multi-cellular plastic trays (50 cells with capacity to hold 90 cm 3 each) filled for C. arabica (REzENDE et al, 2012;ETIENNE et al, 2012;CARVALHO et al, 2013). The use of nodal segments taken from the main orthotropic stem to prepare micro-cuttings was demonstrated by Vos and Snijder (2000).…”

Section: Experiments IImentioning

confidence: 99%

“…Nevertheless, branching can be useful as a preparation to collect vegetative organs (SANSBERRO;MROGINSKI;BOTTINI, 2006). In trees, the shoot apex exerts apical dominance by inhibiting the outgrowth of the axillary sub-apical buds (MüLLER; LEySER, 2011) and, for arabica coffee plants, only orthotropic stems, which are normally under strong apical dominance, can be used to prepare cuttings.…”

Section: Experiments IImentioning

confidence: 99%

Sprouting Induction for Micro-Cutting on in Vitro Cloned Arabica Coffee Plants

Angelo

Ferreira²,

Reis

et al. 2018

C.Sci.

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Vegetative propagation of arabica coffee plants selected by their agronomic value has been accomplished, routinely, for scientific purposes, through somatic embryogenesis and rooting of stem cuttings, in Brazil. Somatic embryogenesis is the election method when a very high number of cloned plants is demanded. Nevertheless, the costs of in vitro multiplication make difficult to explore it commercially. The experiments described herein aimed to amplify the number of in vitro cloned plants, post acclimatization, to reduce costs. Different concentrations of an auxin translocation inhibitor and its association with a cytokinin were applied, as successive pulses, in the 3rd, 8th and 13th months after transference to the greenhouse, on the same sets of Catucaí and Siriema in vitro cloned plants,to induce sprouting. At the 8th month, the experiments with in vitro cloned Catucaí plants were reproduced in the nursery, for comparison. Best results were observed for the association 2,3,5-triiodobenzoic acid (TIBA) 1000 + benzylaminopurine 60 mg.mL-1 applied in the greenhouse, at the 13th month, when 8.5 and 7.0 micro-cuttings above 1 cm in length were produced using sprouts taken from each Catucaí and Siriema acclimatized plant, respectively. Applying this treatment twice a year, and harvesting induced sprouts each six months after the induction treatments, approximately 15 plants per each acclimatized one can be produced. The most important effect of TIBA was the induction of sub-apical sprouting. Greenhouse would be the best environment to apply successive pulses of sprouting inducers to coffee in vitro cloned plants.

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“…Pada kultur in vitro sukrosa pada umumnya diberikan dalam jumlah 2 -5% (George et al 2008). Dalam kultur in vitro kopi melalui embriogenesis somatik, sukrosa diberikan pada kisaran 2 -4% tergantung pada tahap perkembangan embrio (Etienne 2005;Samson et al 2006;Rezende et al 2012).…”

Section: Pendahuluanunclassified

“…Beberapa peneliti terdahulu telah menggunakan berbagai jenis agar pada kultur in vitro kopi. Rezende et al (2012) menggunakan phytagel sebanyak 3,4 g L -1 pada kultur in vitro kopi. Ibrahim et al (2015), Ibrahim et al (2013) bacto agar untuk induksi kalus dan 3,0 g L -1 Gelrite dalam perkembangan embrio somatik kopi.…”

Section: Pendahuluanunclassified