EFISIENSI MEDIA KULTUR DAN APLIKASI TEMPORARY IMMERSION SYSTEM PADA EMBRIOGENESIS SOMATIK KOPI ARABIKA / Efficiency of Culture Media and  Aplication Temporary Immersion Systemon on Somatic Embryogenesis Arabica Coffee

Ibrahim, Meynarti Sari Dewi; Hartati, Rr. Sri; Rubiyo, Rubiyo; Purwito, Agus; Sudarsono, Sudarsono

doi:10.21082/littri.v23n1.2017.45-54

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Cited by 2 publications

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Growth comparison of in vitro-cultured taro Satoimo (Colocasia esculenta var. antiquorum) in solid and liquid media with increase in vitamin B level

Wulansari,

Ermayanti,

Besari

2023

Proceedings of the 9th International Symposium on Innovative Bioproduction Indonesia on Biotechnology and Bioengineering 2022:

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Growth comparison of in vitro-cultured taro Satoimo (Colocasia esculenta var. antiquorum) in solid and liquid media with increase in vitamin B level

Wulansari,

Ermayanti,

Besari

2023

Proceedings of the 9th International Symposium on Innovative Bioproduction Indonesia on Biotechnology and Bioengineering 2022:

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Evaluation of arabica coffee propagation using cell suspension culture

Ibrahim

Tresniawati

2020

IOP Conf. Ser.: Earth Environ. Sci.

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Arabica coffee is highly produced in Indonesia, thus need to be propagated using a powerful approach like cell culture in somatic embryogeneis for providing seedlings. The study aimed to evaluate the use of cell cultures in somatic embryogenesis on several Arabica coffee genotypes. The embryogenic calli (200 mg) were subcultured into solid and liquid regeneration media (half concentration of Murashige and Skoog [MS] added with 2 mg/L of kinetine, 35 g/L of sucrose, 400 mg/L of casein hydrolisate, and mall extract). For the somatic embryos germination, MS media was used by adding it with 0.3 mg/L of BAP and 40 g/L of sucrose. The solid media was added with 2.5 g/L of phytagel. This study was used Factorial Completely Randomized Design (CRD) (genotype and media solidity) with 10 replications.. The results showed that production of the number of somatic embryos is highly depended on the genotypes. The size of somatic embryos, fresh weight, and root length on the torpedo and germination phases in liquid media was higher than solid media. Using liquid media at germination phase isn’t recommended, because it can resulted hyperhydricity.

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EFISIENSI MEDIA KULTUR DAN APLIKASI TEMPORARY IMMERSION SYSTEM PADA EMBRIOGENESIS SOMATIK KOPI ARABIKA / Efficiency of Culture Media and Aplication Temporary Immersion Systemon on Somatic Embryogenesis Arabica Coffee

Cited by 2 publications

References 13 publications

Growth comparison of in vitro-cultured taro Satoimo (Colocasia esculenta var. antiquorum) in solid and liquid media with increase in vitamin B level

Growth comparison of in vitro-cultured taro Satoimo (Colocasia esculenta var. antiquorum) in solid and liquid media with increase in vitamin B level

Evaluation of arabica coffee propagation using cell suspension culture

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