Ethylene synthesis inhibition effects on oxidative stress and in vitro conservation of Lippia filifolia (Verbenaceae)

Abstract

This study aimed to investigate the effects of ethylene biosynthesis inhibitors on oxidative metabolisms and the in vitro conservation of Lippia filifolia, using the lipid peroxidation index (TBARS), antioxidative enzymes and pigments as biomarkers. We found that EDTA, sodium thiosulfate (STS) and especially Co had protective effects on oxidative stress in tissues cultured in vitro, resulting in a delay of the senescence and the reduction of subcultures frequency, contributing to the germplasm conservation of this species.Keywords: ethylene inhibitors, TBARS, ROS, micropropagation, germplasm conservation. Efeitos da

Slow-grown in vitro conservation of Heliconia champneiana cv. Splash under different light spectra

“…The occurrence of yellowing leaves is frequently associated with stress caused by the in vitro environment resulting from the scarcity of nutrients in the culture medium and/or difficulties in gaseous exchanges, which accelerates the processes of senescence. These characteristics are commonly observed in plants that exceed the maximum time spent in vitro (Pimenta et al, 2013;Kevers et al, 2004).…”

Enzymatic activity and elicitor of phytoalexins of Lippia sidoides Cham. and endophytic fungi

“…In the work of Pimenta et al (2013), these enzymes were detected in the plant, Lippia filifolia, where they verified the effect of the inhibition of ethylene synthesis on oxidative stress. The expression of the genes encoding these enzymes is dependent on several types of abiotic and biotic stresses emphasizing the importance and complexity of understanding the mechanisms involved and their complexity.…”

Effects of different light spectra on the slow-grown in vitro storage and quality of banana plantlets cv. Prata Catarina (AAB)

“…In the plantlets, no yellowing or leaf necrosis, a typical symptom of in vitro stress caused by the gas-saturated in vitro environment and/or nutrient shortage in the culture medium, was observed (Rodrigues et al 2018 ; Pimenta et al 2013 ). In a restricted environment without regular subculture, gas accumulation due to explant growth can be detrimental to plant development in vitro.…”