Effects of different light spectra on the slow-grown in vitro storage and quality of banana plantlets cv. Prata Catarina (AAB)

Abstract

Continues subculturing of in vitro plant subcultures is essential for commercial micropropagation and tissue culture research. In unusual situations, the subcultures can be delayed, and the slow-growth in vitro storage technic could be applied to reduce the loss of plant material. The present study aimed to evaluate the slow-growth in vitro storage of banana plantlets (‘Prata Catarina’; group AAB) under different light spectra. Shoot cultures in MS medium without plant growth regulators were maintained under blue (B), red (R), red plus blue (R2B), and white (CW) light spectra (25 °C ± 2 °C; 50 µmol m −2 s −1 ) for up to 140 days. The plantlets maintained under the R, CW, and R2B spectra suffered severe root damage and browning after 140 days of in vitro slow-growth storage (SGS). All plantlets maintained under the B spectrum survived after 140 days of in vitro SGS and showed little browning.

Biotechnological interventions in banana: current knowledge and future prospects

“…Browning of the medium occurs due to the oxidation of phenolic compounds produced by banana explants resulting in reduced cell division and plant growth ( Rodrigues et al., 2022 ). These exudates form a barrier around the tissue which prevents nutrient uptake ( Tabiyeh et al., 2005 ; Krishna et al., 2008 ).…”

Composition of media for in vitro slow growth storage (sgs) of Aglaonema

“…In vitro storage using the Slow Growth Storage technique is carried out in several ways, including the use of growth retardant and osmotic compounds (Dewi et al, 2020;Syahid, 2020), encapsulation (Hassanen, 2021), storage in dark conditions and control of the light spectrum (Rodrigues et al, 2022), modification of the growing media by reducing sucrose levels and concentration of primary media , a combination of diluting media with growth inhibitory substances (Syahid, 2021;Syahid and Parlindung, 2023) and the use of a specific light spectrum (Rodrigues et al, 2022). Modifying the media composition by reducing the content of sugars, minerals, growth regulators, or osmotic agents such as sorbitol and mannitol can inhibit cell division and significantly limit callus formation and shoot development.…”

Integrated Analysis of Morphological, Physiological, Anatomical and Molecular Responses of Cassava Seedlings to Different Light Qualities

“…Light quality is a critical environmental factor in tissue culture. Recently, reports have studied the impact of various light spectra on the growth of plants in vitro in many species, including pitaya [ 17 ], potato [ 18 ], Cinchona officinalis [ 19 ], Leucojum aestivum [ 22 ], Swertia chirayita [ 23 ], Pyrus communis [ 24 ], Valencia orange [ 25 ] and banana [ 26 ]. However, to date, no such study has been conducted on in vitro-grown cassava plants.…”