The effect of intermittent cryotherapy on the activities of citrate synthase and lactate dehydrogenase in regenerating skeletal muscle

Abstract

ABSTRACT

Cryotherapy Reduces Inflammatory Response Without Altering Muscle Regeneration Process and Extracellular Matrix Remodeling of Rat Muscle

“…The animals were maintained in a horizontal position on a plastic table, and the ankle of right hindlimb was maintained by tape for the exposition of the TA muscle skin. The sessions of cryotherapy consisted of the application of a plastic pack filled with crushed ice, maintained by the tape directly on the skin of the right TA muscle 22 23 . The first cryotherapy treatment was performed immediately after the TA injury (three sessions of 30 minutes, applied every 2 h).…”

“…Cryotherapy is applied in clinical practice with a duration of 20 to 30 minutes, at least 3 times per day in the first 72 hours after soft injury 18 19 . Some studies have demonstrated that cryotherapy minimizes secondary hypoxic injury through the reduction of cellular metabolism and injury area 17 20 21 22 23 24 . Nuno and colleagues 22 also showed that intermittent cryotherapy reduced injury area approximately 25% compared to untreated rat muscle.…”

“…Collectively, few studies have evaluated the effects of cryotherapy on muscle regeneration with intermittent and clinical-like protocols related to those used in humans 4 22 23 . Moreover, no study used cryotherapy for 20 minutes in the first 72 hours after muscle injury, as recommended in clinical practice.…”

Efeitos da crioterapia na modulação do dano oxidativo resultante de lesão muscular em ratos

“…Na colocação do saco de gelo sobre a pele, era realizada uma compressão com fita crepe, a qual foi fixada sempre pela mesma pessoa a fim de evitar compressões diferenciadas (10,54 …”

“…Uma possível razão para esse resultado pode ser que o dano causado pela criolesão seja maior do que o dos outros mecanismos, e assim a atividade enzimática da SOD foi ativada em maiores proporções. Oliveira e colaboradores (2013) descrevem que a baixa temperatura do mecanismo da criolesão pode alterar a estrutura quartenária das proteínas (54,87).…”