Efeitos de operações sobre o baço no lipidograma de ratas

Petroianu, Andy; Veloso, Denny Fabrício Magalhães; Costa, Gustavo Rocha; Alberti, Luiz Ronaldo

doi:10.1590/s0104-42302006000100024

Cited by 21 publications

(16 citation statements)

References 14 publications

(37 reference statements)

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“…34 The inferior pole functions better after 90 days following STPI. 8 Vascular neoformation in the inferior pole deserves further investigation. The inferior pole, although not having been as efficacious as the integral spleen, was better off than the auto-implants because: a-it permitted more survivals; b-evoked better platelet and Howell Jolly corpuscle clearing, and did not evoke such a marked falling of IgM ; c-The macro and microscopic appearance of the inferior pole presented itself more viable than that of the autoimplants.…”

Section: Discussionmentioning

confidence: 99%

“…Complicating infections following spleen removal, have also been observed in experimental animals. 3,4 Besides these, alterations in lipid metabolism following splenectomy have been reported in human subjects 5 and experimental animals 6,7,8 , capable of causing atherosclerosis. 6 For these reasons, conservative spleen operations including vascular occlusions 9,10 , splenorrhaphies 11 , partial splenectomies 12,13,14 and auto-implants.…”

Section: Introductionmentioning

confidence: 99%

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Preservative spleen surgery and hyperbaric oxygen therapy

Paulo

Cintra³

et al. 2007

Acta Cir. Bras.

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Purpose :To assess functional and morphological aspects of spleen auto-implants and of the splenic inferior pole of rats, post-operatively treated or not with hyperbaric oxygen, as well as the survival of these animals, were studied. Methods: Seventy-eight male Wistar rats, weighing between 192 and 283 g ( 238,3 ± 9,6g), were randomly distributed into three groups: Group1-(n=20), spleen manipulation; group 2-(n=36), spleen auto-implantation; group3-(n= 22), subtotal splenectomy preserving the inferior pole. Each group was subdivided as follows: subgroup a, not submitted to hyperbaric oxygen therapy: 1a(n=10), 2a(n=21), 3a(n= 13); subgroup b, submitted to the therapy: 1b(n=10), 2b(n=15), 3b(n=9). Blood was collected pre-operatively and 11 days after surgery, for the estimation of lipids and immunoglobulins and the counting of platelets and Howell-Jolly corpuscles. The spleen and remains were taken for histological study. Results : The number of surviving animals was significantly higher in groups 1(p<0,01) and 3(p<0,05) relative to those of subgroup 2a. Total cholesterol and the LDL fraction increased significantly in subgroup 2a (p<0,01) and 3a (p<0,05), and remained unaltered in subgroups 2b e 3b. IgM decreased more significantly in subgroup 2 than in subgroup 3 (p<0,001 vs p<0,01). The increase of platelet numbers and the appearance of Howell Jolly corpuscles was smaller in subgroup 2b compared to subgroup 2a , and in group 3 compared to group aqui-> 2. The macro and microscopic appearance in subgroup 2b were more viable than in subgroup 2a, and that of group 3 more viable than in group 2. The survival of the animals carrying their whole spleen or its inferior pole was more frequent than that of the auto-implanted animals. Conclusion : Functionality and viability of the whole spleen or of its inferior pole, were better than in the auto-implanted animals. Hyperbaric oxygentherapy contributed to increased survival frequency of auto -implanted animals, and to improve the functionality and viability of the auto-implants and the function of the inferior splenic pole, and did not interfere in animals carrying their whole spleen. Key words: Splenectomy/methods.Spleen/metabolism. Adverse effects/splenectomy. Oxygen inhalation therapy/methods RESUMO Objetivo : Estudar aspectos funcionais e morfológicos dos auto-implantes esplênicos e do pólo inferior do baço de ratos, tratados ou não com oxigênio hiperbárico no pós-operatório, e a sobrevida desses animais. Métodos: Foram operados 78 ratos, machos, Wistar, pesando entre 192 g e 283 g ( M.A 238,3 ± 9,6) , distribuídos aleatoriamente em três grupos : 1-(n=20) , manipulação do baço; 2-(n=36), auto-implante esplênico; 3(n= 22), esplenectomia subtotal com preservação do pólo inferior. Cada grupo foi dividido em dois subgrupos: a-não submetido à oxigenoterapia hiperbárica: 1a(n=10), 2a(n=21), 3a(n= 13); b-submetido: 1b(n=10), 2b(n=15), 3b(n=9). O sangue foi colhido para dosagem dos lípides e imunoglobulinas e contagem das plaquetas e dos corpúsculos de Howell-Jolly n...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Preservative spleen surgery and hyperbaric oxygen therapy

Paulo

Cintra³

et al. 2007

Acta Cir. Bras.

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“…The Spleen, which stands out for the filtration and hematopoietic functions, also participates in metabolic control 2 . Persons undergoing splenectomy after splenic trauma have a higher incidence of coronary artery atherosclerosis, probably caused by lipid disorders 3 .…”

mentioning

confidence: 99%

“…Patients with myeloproliferative diseases associated with hypersplenism showed a decrease in serum total cholesterol and its fraction of low-density lipoprotein (LDL) 6 . After splenectomy, or when the disease was controlled, the reversal of hypocholesterolemia occurred 2 . In experimental studies, total splenectomy was accompanied by high levels of cholesterol and triglycerides, with elevated LDL cholesterol 7 .…”

mentioning

confidence: 99%

“…However, those who study the spleen function in lipid metabolism are rare. There is no consensus on the actual mechanism through which the spleen controls serum lipid levels 2,13,14 . The regeneration of the spleen function seems to have a direct relationship with implanted splenic mass 15 , so we decided to perform the allograft spleen in rats, preserving its original functioning spleen, causing the "double spleen".…”

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confidence: 99%

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Effects of splenic allograft in lipid profile of non-splenectomized rats: the immune and metabolic role of the "double spleen"

Gonçalves

Yamaki

Feijó

et al. 2014

Rev. Col. Bras. Cir.

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Gonçalves Gonçalves Gonçalves Gonçalves GonçalvesEffects of splenic allograft in lipid profile of non-splenectomized rats: the immune and metabolic role of the "double spleen"Original Article Original Article Original Article Original Article Original Article Effects of splenic allograft in lipid profile of non-splenectomized Effects of splenic allograft in lipid profile of non-splenectomized Effects of splenic allograft in lipid profile of non-splenectomized Effects of splenic allograft in lipid profile of non-splenectomized Effects of splenic allograft in lipid profile of non-splenectomized rats: the immune and metabolic role of the "double spleen" rats: the immune and metabolic role of the "double spleen" rats: the immune and metabolic role of the "double spleen" rats: the immune and metabolic role of the "double spleen" rats: the immune and metabolic role of the "double spleen"Efeitos do aloenxerto esplênico no lipidograma de ratos não esplenectomizados: Efeitos do aloenxerto esplênico no lipidograma de ratos não esplenectomizados: Efeitos do aloenxerto esplênico no lipidograma de ratos não esplenectomizados: Efeitos do aloenxerto esplênico no lipidograma de ratos não esplenectomizados: Efeitos do aloenxerto esplênico no lipidograma de ratos não esplenectomizados: papel imunológico e metabólico do "baço duplo" papel imunológico e metabólico do "baço duplo" papel imunológico e metabólico do "baço duplo" papel imunológico e metabólico do "baço duplo" papel imunológico e metabólico do "baço duplo" (1), total splenectomy group (2), splenectomy and implantation of allograft group (3) and double spleen group (4). Each group was subdivided into two subgroups: A and B, based on the death of the animals after 30 or 120 days of monitoring. The procedures in groups 2, 3 and 4 were made simultaneously, and splenectomized animals, groups 2 and 3 were donors, respectively, for the animals of groups 3 and 4. In group 4 the spleen was preserved and the animals received implants from the spleens of rats from group 3. The regeneration of splenic tissue was evaluated by macroscopic and microscopic analyzes of the grafts and own spleens, as well as with measurements of VLDL, HDL, LDL, total cholesterol and triglycerides. Results Results ResultsResults Results: after 120 days, Group 4 showed levels of total cholesterol and LDL lower than the other groups. Group 1 had higher levels of lipids. Conclusion Conclusion Conclusion Conclusion Conclusion: The technique of double spleen was effective in the control of lipid metabolism, corroborating the function of the spleen as a reserve of lipids.

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