Regeneração in vitro de Passiflora suberosa a partir de discos foliares

Abstract

A regeneração in vitro de plantas de Passiflora suberosa foi obtida a partir de discos foliares. Folhas foram retiradas de plantas germinadas em casa de vegetação, imersas em solução comercial de hipoclorito de sódio (3:1), durante 20 minutos. Discos foliares de 0,5 cm de diâmetro, com a face adaxial em contato com o meio de cultura, foram introduzidos em placa de petri. Organogênese foi obtida utilizando-se meio MS acrescido de 0,5 ou 1,0 mg L-1 de BAP (6-benzilaminopurina). Após 4 a 8 semanas, observou-se formação de calos nas bordas dos discos foliares. Os calos formados foram transferidos para meio MSM, acrescido de 1,0 mg L-1 de GA3 (ácido giberélico), sob fotoperíodo de 16 horas. Desenvolvimento de gemas adventícias foi obtido a partir dos calos, sendo estas alongadas e enraizadas no mesmo meio de cultura, após periódicas repicagens, e aclimatadas em casa de vegetação.

In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast.

“…The formation of buds in leaf discs was observed after the formation of calluses at the explant ends. The indirect organogenesis in P. cincinnata occurred only in culture medium containing 6-BA regulator, which was also observed in internode segments of P. edulis f. flavicarpa (Biasi et al 2000) and in leaf discs of Passiflora suberosa (Monteiro et al 2000). In P. edulis f. flavicarpa, the direct organogenesis in leaf discs described by Dornelas and Vieira (1994), at 28 days of culture in MS medium with 1.0 mg. L -1 de 6-BA, had a regeneration response of 41.7%.…”

“…Several works on in vitro regeneration have been published on a few Passiflora species (Dornelas and Vieira 1994;Appezzato-da-Glória et al 1999;Monteiro et al 2000;Hall et al 2000;Biasi et al 2000;Reis et al 2003;Becerra et al 2004). In order to fully use these tools, in vitro plant regeneration processes can be assayed and characterized by histological and morphological analysis of plant material.…”

Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit

“…1B). Monteiro et al (2000b) also observed bud formation when they cultured leaves of P. suberosa in vitro in MS medium in the dark at 26 ± 2°C for four weeks and then transferred calli with organogenic aspects to MSM medium with 2.89 µm GA 3 under light. However, when tissues of P. suberosa were incubated in the dark without a subsequent period in the light, no significant shoot formation occurred (Garcia et al 2011).…”

Search for alkaloids on callus culture of Passiflora alata

“…In vitro propagation of Passiflora has shown high contamination levels and difficulty in breaking the dormancy of the seeds (Monteiro et al, 2000, Passos et al, 2004. Therefore, in this work, the seeds of P. alata were treated with gibberellic acid after disinfection, and presented low germination levels (less than 5%).…”

“…Therefore, this work dealt with inducing the callus and cell culture of P. alata and the investigation of in vitro alkaloid production, with and without L-tryptophan feeding. The cell culture of Passiflora was revised (Drew 1997), and some authors, using several explants such as endosperm and zygotic embryo (Guzzo et al, 2004), internode segments (Biasi et al, 2000) and leaf discs (Monteiro et al, 2000) carried out organogenesis. The culture of isolated protoplasts (naked cells), followed by reproducible plant regeneration, was also studied (Davey et al, 2006).…”