Isolation of Arcobacter spp from the milk of dairy cows in Brazil

Pianta, Celso; Passos, Daniel Thompsen; Hepp, Diego; Oliveira, Sérgio José de

doi:10.1590/s0103-84782007000100027

Cited by 29 publications

(23 citation statements)

References 22 publications

(17 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although raw milk has previously been identified as a risk factor for human campy lobacteriosis (13), there is limited data concerning raw milk as a source of Arcobacter species. Although different rates of Arcobacter spp.-positive raw milk samples have been found in Turkey (6%) (3), Northern Ireland (46%) (14), and Brazil (3%) (11), no estimates have been made of the occurrence of Arcobacter spp. in Scandinavia.…”

mentioning

confidence: 99%

Arcobacter Species and Their Pulsed-Field Gel Electrophoresis Genotypes in Finnish Raw Milk during Summer 2011

Revez

Huuskonen

Ruusunen

et al. 2013

Journal of Food Protection

View full text Add to dashboard Cite

The aim of this study was to investigate the occurrence of Arcobacter species in raw milk in Finland. A total of 177 raw milk samples, each from a separate farm, were examined from June to August 2011. Arcobacter species were isolated using an enrichment and selective detection procedure. Overall, 26 (15 % ) of the 177 samples yielded Arcobacter spp. Samples from 25 farms were positive for Arcobacter butzleri and from 1 farm for Arcobacter cryaerophilus. Moreover, both Arcobacter butzleri and A. cryaerophilus were recovered from 1 positive sample. To evaluate a possible genetic variability, one strain of A. butzleri from each farm and the A. cryaerophilus sample were analyzed by pulsed-field gel electrophoresis. Genotyping revealed that Arcobacter spp. populations are heterogeneous, and no dominant clone has spread in the investigated samples. Our study is the first report on the isolation of both A. butzleri and A. cryaerophilus in raw milk in Finland. Based on our findings, the presence of Arcobacter species in raw milk may pose a potential hazard for human health, in particular for consumers who prefer drinking unpasteurized milk.

show abstract

mentioning

confidence: 99%

Arcobacter Species and Their Pulsed-Field Gel Electrophoresis Genotypes in Finnish Raw Milk during Summer 2011

Revez

Huuskonen

Ruusunen

et al. 2013

Journal of Food Protection

View full text Add to dashboard Cite

show abstract

“…So far, thirteen species have been identified from different sources within the genus Arcobacter: Arcobacter butzleri, A. cryaerophilus ( [5,10]. Among these species A. butzleri, A. cryaerophilus and A. skirrowii have been associated with enteritis and, occasionally, bacteremia in humans [17] and abortion and mastitis in cattle and swine [6,18,22]. However, Arcobacter has been isolated from large numbers of clinically healthy animals [2,20,26,27] and various environmental sources such as water and sludge [4,7].…”

mentioning

confidence: 99%

<i>In vitro</i> Antibacterial Susceptibility of <i>Arcobacter butzleri</i> Isolated from Different Sources

et al. 2012

View full text Add to dashboard Cite

ABSTRAcT. The objective of this study was to determine the antibiotic susceptibility of Arcobacter spp. isolated from various sources. Seventy Arcobacter spp. isolates were tested for their susceptibility to 13 antimicrobial agents. Antimicrobial susceptibility testing was performed by using the agar disc diffusion method on Mueller-Hinton agar supplemented with 5% defibrinated sheep blood. The antibiotics tested included enrofloxacin, erythromycin, streptomycin, gentamycin, rifampin, tetracycline, ampicillin, trimethoprim/ sulfamethoxazole, nalidixic acid, danofloxacin, amoxycillin-clavulonic acid, cefuroxime-sodium, neomycine. Although all the arcobacters tested were susceptible to gentamycin, resistance to three or more antibacterial agents (especially, trimethoprim/sulfamethoxazole, cefuroxime-sodium and rifampin) was observed. A. butzleri isolates were found to be resistant to amoxycillin+clavulonic acid, nalidixic acid and ampicillin, at the rate of 20%, 44.28% and 78.57% respectively. In conclusion, gentamycin, streptomycin and tetracycline may be suitable antibiotics for the treatment or control of disease caused by Arcobacter spp. in veterinary and human medicine. KEY WORDS: agar disc diffusion, antibacterial susceptibility, Arcobacter spp.

show abstract

“…Outra bactéria Gram negativa também isolada em casos de mastites no Brasil foi Arcobacter spp. no Rio Grande do Sul (Pianta et al 2007). …”

Section: Quadro 1 Frequência Relativa (%) Dos Microrganismos Isoladounclassified

“…Grandes regiões e espécies % de resistência a diferentes antimicrobianos em exploração Nordeste A (Krewer et al 2013) amoxicilina (67,4%) ampicilina (67%) penicilina (66%) B (Coutinho et al 2006) penicilina (80%) ampicilina (80%) neomicina (80%) B (Neves et al 2010) penicilina (66,67%) ampicilina (63,89%) B (Peixoto et al 2010a) ácido nalidíxico (78,26%) neomicina (43,26%) amoxicilina (38.51) Sudeste A (Ribeiro et al 2009) penicilina (53,5%) ampicilina (41,6%) neomicina (38,6%) B amoxicilina (79,5) penicilina (76,8%) B penicilina (87,5%) ampicilina (75%) neomicina (62,5%) C (Pizauro et al 2014) neomicina (32,5%) enrofloxacina (28,9%) penicilina (25,3%) Centro-Oeste A (Fontana et al 2010) penicilina (100 %) oxacilina (100 %) ampicilina (100 %) tados no Quadro 1 somente dois deles (Pianta et al 2007, Mesquita et al 2012) utilizaram esta ferramenta, sendo ambos trabalhos realizados na região Sul do país. A reação em cadeia da polimerase (PCR) apresenta alta especificidade e sensibilidade na detecção do DNA do patógeno causador da doença, além da rapidez do diagnóstico (<6 horas), permitindo a identificação de patógenos que não crescem em técnicas convencionais de cultura ou microrganismos de difícil crescimento (Koskinen et al 2009).…”

Section: Quadro 2 Perfil De Resistência a Antimicrobianos De Diferenunclassified

Mastites em ruminantes no Brasil

et al. 2016

View full text Add to dashboard Cite

Resumo: A mastite é uma doença complexa e considerada uma das principais causas de perdas à indústria leiteira mundial. Objetivou-se com esta revisão compilar informações dos últimos dez anos sobre a mastite em ruminantes no Brasil. A prevalência da mastite subclínica chega a 48,64% na espécie bovina, 30,7% na espécie caprina, 31,45% na espécie ovina e 42,2% na espécie bubalina, destacando-se a etiologia por Staphylococcus spp. Os fatores de risco associados à ocorrência de mastite estão relacionados a problemas no saneamento ambiental e ao manejo dos animais. As bactérias isoladas do leite mastítico apresentam maior percentual de resistência a penicilina, ampicilina, amoxicilina e neomicina e a utilização de técnicas moleculares no diagnóstico dos agentes causadores de mastites no país, ainda é escassa o que dificulta a obtenção de um diagnóstico mais rápido, sensível e específico.

show abstract

Isolation of Arcobacter spp from the milk of dairy cows in Brazil

Abstract: Bacteriologic examinations were performed on 188 milk samples collected from cows from 11 farms for diagnosis of mastitis in three cities of Rio Grande do

Cited by 29 publications

References 22 publications

Arcobacter Species and Their Pulsed-Field Gel Electrophoresis Genotypes in Finnish Raw Milk during Summer 2011

Arcobacter Species and Their Pulsed-Field Gel Electrophoresis Genotypes in Finnish Raw Milk during Summer 2011

<i>In vitro</i> Antibacterial Susceptibility of <i>Arcobacter butzleri</i> Isolated from Different Sources

Mastites em ruminantes no Brasil

Contact Info

Product

Resources

About