Comparação entre o uso de fio inabsorvível (polipropileno) e fio absorvível (poliglactina 910) na fixação de prótese de polipropileno em correção de defeitos músculo-aponeurótico da parede abdominal: estudo experimental em ratos

PURPOSE:To investigate the effects of alloxan diabetes on the abdominal wall healing of rats undergoing laparotomy. METHODS:Ninety-six male Wistar rats weighing between 200 and 300 grams, divided into two groups: non-diabetic group (G1) and another with untreated diabetes (G2). Three months after diabetes induction, the animals underwent a 5cm-long-laparotomy and 5.0 nylon monofilament suture. After the surgery, 12 animals from each group were euthanized on days 4, 14, 21 and 30 corresponding to the moments M1, M2, M3 and M4. In each moment a fragment of the abdominal wall containing the scar was removed for tensile strength measurement, histological and morphometric study. Clinical and biochemical parameters were also analyzed. RESULTS:G2 animals showed parameters compatible with severe diabetes and decreased plasma levels of insulin. The tensile strength in G2 was significantly smaller in M2 and M4, with a tendency to fall in the other two. Through light microscope, diabetic animals showed more difficulty to increase collagen density and contraction. G2 animals showed high cellularity of fibroblasts in later healing moments, with collagen thinning in M2 and M4. CONCLUSION:The abdominal wound healing in untreated diabetic animals was altered and led to a higher incidence of dehiscence and infections.Key words: Diabetes Mellitus. Alloxan. Tensile Strength. Wound Healing. Histology. Rats. Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats

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“…The abdominal wall has been further studied to analyze the healing of sutures or synthetic materials such as surgical meshes 7,11,12 .…”

Section: Discussionmentioning

confidence: 99%

Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats

et al. 2014

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“…The experimental model used here was effective in reproducing incisional hernia in all cases and was able to simulate the conditions encountered after median laparotomy, in contrast with the model produced by the removal of a muscle-aponeurotic fragment from the abdominal wall 10 , which is frequently reported in the literature 11,12 .…”

Section: Discussionmentioning

confidence: 99%

Comparative study between two techniques of incisional hernia repair with polypropylene mesh in rabbits

et al. 2010

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Purpose:To compare two different incisional hernia repair techniques (repair with a polypropylene mesh reinforcement on the peritonium-aponeurosis versus polypropylene mesh sutured to the borders of the hernial ring as a bridge) in rabbits. Methods: Incisional hernia was experimentally developed through a 4-cm median incision in 60 rabbits. After 30 days, half of the animals were operated for primary wall closure and placement of a polypropylene mesh reinforcement, while the other half had a polypropylene mesh sutured to the borders of the hernial ring as a bridge. Clinical development, scar breaking strength, as well as gross, microscopic and morphometric parameters were evaluated in all animals 30, 60, and 90 days after repair. Results: No significant differences in breaking strength or histological parameters were observed between groups at any time point studied. No statistical difference regarding complications was detected, although denser and firmer adhesions to the abdominal wall were seen after the mesh was placed as a "bridge". Conclusions: No significant differences between the incisional hernia repair techniques assessed were observed regarding breaking strength, and histological and morphometric parameters. The number of complications was similar in both study groups. However, adhesion of abdominal cavity organs to the scar area was much denser after the placement of a mesh to bridge the defect. Key words: Hernia, Ventral. Polypropylenes. Surgical Mesh. Rabbits. RESUMOObjetivo: Comparar duas técnicas de tratamento da hérnia incisional em coelhos utilizando a tela de polipropileno apoiando um reforço peritônio -aponeurótico ou suturada nas bordas do anel herniário 'em ponte". Métodos: Foram operados 60 coelhos para a produção de hérnia incisional, em uma incisão mediana de 4 centímetros. Após 30 dias, metade dos animais foram operados com o fechamento primário da parede, com colocação de uma tela de polipropileno apoiando o reforço e a outra metade dos animais com a colocação da tela suturada nas bordas do anel herniário "em ponte". Os animais foram avaliados com 30 (M1), 60 (M2)e 90 (M3) dias de pós-operatório. Os parâmetros analisados foram a evolução clínica, análise da força de ruptura da cicatriz, estudo macroscópico, análise microscópica e morfométrica. Resultados: Não foram observadas diferenças significantes com relação a força de ruptura e estudos histológicos nos dois grupos e vários momentos estudados. Não houve diferença estatística com relação às complicações, embora os animais que receberam a tela "em ponte" tiveram aderências mais firmes e intensas à parede abdominal. Conclusões: As duas técnicas utilizadas para correção da hérnia incisional em coelhos não mostraram diferenças significantes quanto a força de ruptura, análise histológica e morfométrica. O número de complicações foi semelhante, porém a aderência de órgãos da cavidade abdominal à área de cicatriz foi muito mais intensa no grupo em que a tela foi colocada "em ponte". Descritores: Hérnia Ventral. Polipropilenos.Tel...

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“…There are several techniques for their correction, as for example primary suture of the wall 5 ; however the best results for this pathology are obtained by using prostheses reinforcement, with which the chances of relapse vary between 3 to 17% 6 .…”

Section: Introductionmentioning

confidence: 99%

Effect of copaiba oil on correction of abdominal wall defect treated with the use of polypropylene/polyglecaprone mesh

Yasojima¹,

Teixeira²,

Houat³

et al. 2013

Acta Cir. Bras.

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PURPOSE:To evaluate the effects of copaiba oil on the correction of abdominal defect treated with the use of polypropylene/ polyglecaprone mesh in rats. METHODS:A defect in the abdominal wall was created and corrected with polypropylene/polyglecaprone mesh in 36 rats. They were randomly distributed into three groups: control, copaiba by oral administration (gavage) and copaiba oil dip in the mesh. Euthanasia was performed after seven, 14 and 21 post-operative days. The healing process was analyzed regarding the meshes and macroscopic and microscopic aspects. RESULTS:All animals had abdominal adhesions, which were smaller in the copaiba (gavage) group (p<0.05). In microscopy, all animals had an acute inflammation stage and the inflammatory response was best characterized by foreign body-type granulomas around the mesh fragments, which was not found in the mesh fragments within the copaiba dip group. There was a greater area of necrosis and fibrosis in the copaiba dip group compared to the control group (p<0.05). The copaiba (gavage) group had a greater quantity of collagen fibers compared to the control group. CONCLUSION:Copaiba oil administered by gavage decreased the amount of abdominal adhesions, besides accelerating the process of collagen fibers formation, without damages within the early stages of healing. However, when used by dip directly on the mesh, it had corrosive effects compromising the healing process of the abdominal wall.

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Comparação entre o uso de fio inabsorvível (polipropileno) e fio absorvível (poliglactina 910) na fixação de prótese de polipropileno em correção de defeitos músculo-aponeurótico da parede abdominal: estudo experimental em ratos

Cited by 13 publications

References 34 publications

Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats

Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats

Comparative study between two techniques of incisional hernia repair with polypropylene mesh in rabbits

Effect of copaiba oil on correction of abdominal wall defect treated with the use of polypropylene/polyglecaprone mesh

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